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Laser capture microdissection in forensic research: a review

Mado Vandewoestyne UGent and Dieter Deforce UGent (2010) INTERNATIONAL JOURNAL OF LEGAL MEDICINE. 124(6). p.513-521
abstract
In forensic sciences, short tandem repeat (STR) analysis has become the prime tool for DNA-based identification of the donor(s) of biological stains and/or traces. Many traces, however, contain cells and, hence, DNA, from more than a single individual, giving rise to mixed genotypes and the subsequent difficulties in interpreting the results. An even more challenging situation occurs when cells of a victim are much more abundant than the cells of the perpetrator. Therefore, the forensic community seeks to improve cell-separation methods in order to generate single-donor cell populations from a mixed trace in order to facilitate DNA typing and identification. Laser capture microdissection (LCM) offers a valuable tool for precise separation of specific cells. This review summarises all possible forensic applications of LCM, gives an overview of the staining and detection options, including automated detection and retrieval of cells of interest, and reviews the DNA extraction protocols compatible with LCM of cells from forensic samples.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (review)
publication status
published
subject
keyword
Sexual assault, Forensics, Laser capture microdissection, Cell separation techniques, POLYMERASE-CHAIN-REACTION, SEXUAL ASSAULT EVIDENCE, IN-SITU HYBRIDIZATION, EPITHELIAL-CELLS, CHORIONIC VILLI, DNA, SPERM, SPERMATOZOA, STAINS, SEPARATION
journal title
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
Int. J. Legal Med.
volume
124
issue
6
pages
513 - 521
Web of Science type
Review
Web of Science id
000282826800001
JCR category
MEDICINE, LEGAL
JCR impact factor
2.939 (2010)
JCR rank
1/13 (2010)
JCR quartile
1 (2010)
ISSN
0937-9827
DOI
10.1007/s00414-010-0499-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1019377
handle
http://hdl.handle.net/1854/LU-1019377
date created
2010-08-09 11:41:34
date last changed
2017-07-24 13:04:40
@article{1019377,
  abstract     = {In forensic sciences, short tandem repeat (STR) analysis has become the prime tool for DNA-based identification of the donor(s) of biological stains and/or traces. Many traces, however, contain cells and, hence, DNA, from more than a single individual, giving rise to mixed genotypes and the subsequent difficulties in interpreting the results. An even more challenging situation occurs when cells of a victim are much more abundant than the cells of the perpetrator. Therefore, the forensic community seeks to improve cell-separation methods in order to generate single-donor cell populations from a mixed trace in order to facilitate DNA typing and identification. Laser capture microdissection (LCM) offers a valuable tool for precise separation of specific cells. This review summarises all possible forensic applications of LCM, gives an overview of the staining and detection options, including automated detection and retrieval of cells of interest, and reviews the DNA extraction protocols compatible with LCM of cells from forensic samples.},
  author       = {Vandewoestyne, Mado and Deforce, Dieter},
  issn         = {0937-9827},
  journal      = {INTERNATIONAL JOURNAL OF LEGAL MEDICINE},
  keyword      = {Sexual assault,Forensics,Laser capture microdissection,Cell separation techniques,POLYMERASE-CHAIN-REACTION,SEXUAL ASSAULT EVIDENCE,IN-SITU HYBRIDIZATION,EPITHELIAL-CELLS,CHORIONIC VILLI,DNA,SPERM,SPERMATOZOA,STAINS,SEPARATION},
  language     = {eng},
  number       = {6},
  pages        = {513--521},
  title        = {Laser capture microdissection in forensic research: a review},
  url          = {http://dx.doi.org/10.1007/s00414-010-0499-4},
  volume       = {124},
  year         = {2010},
}

Chicago
Vandewoestyne, Mado, and Dieter Deforce. 2010. “Laser Capture Microdissection in Forensic Research: a Review.” International Journal of Legal Medicine 124 (6): 513–521.
APA
Vandewoestyne, M., & Deforce, D. (2010). Laser capture microdissection in forensic research: a review. INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 124(6), 513–521.
Vancouver
1.
Vandewoestyne M, Deforce D. Laser capture microdissection in forensic research: a review. INTERNATIONAL JOURNAL OF LEGAL MEDICINE. 2010;124(6):513–21.
MLA
Vandewoestyne, Mado, and Dieter Deforce. “Laser Capture Microdissection in Forensic Research: a Review.” INTERNATIONAL JOURNAL OF LEGAL MEDICINE 124.6 (2010): 513–521. Print.