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Addition of insulin like growth factor-1 during maturation and culture enhances blastocyst rate and reduces apoptosis in bovine blastocysts derived from heat stressed oocytes

Mohamed Ahmed MM Shehab El Deen UGent, Jo Leroy, Leen Vandaele UGent, Dominiek Maes UGent, Geert Opsomer UGent, Moustafa Fadel and Ann Van Soom UGent (2010) REPRODUCTION IN DOMESTIC ANIMALS. 45(suppl. 3). p.71-71
abstract
Heat stress (HS) reduces bovine oocyte developmental competence. Previous research showed that the addition of insulin like growth factor-1 (IGF-1) during maturation reduced apoptosis in heat stressed oocytes with no apparent effect on blastocyst rates. We hypothesized that adding IGF-1 during maturation and culture may enhance blastocyst rate and decrease apoptosis in blastocysts derived from heat stressed oocytes. During the first 12 h of maturation, HS (40.5C) was applied while IGF-1 (100 ng/ml) was added during maturation (M), during culture (C) or during both (MC). Two control groups were included (negative-control: no HS nor IGF-1 and HS-control: HS but no IGF-1). Blastocyst rates were 27.9 ± 2.1a, 10.9 ± 1.2b, 9.7 ± 1.2b, 18.4 ± 2.4c, and 15.3 ± 1.9bc % in negative-control, HS- ontrol, M, C and MC groups, respectively (a,b,c p < 0.05). Apoptotic cell ratios (ACR) were 9.7 ± 1a, 11.8 ± 1.2a, 11.6 ± 2a, 7.8 ± 0.8b and 5.9 ± 0.7c % in negative-control, HS-control, M, C and MC groups, respectively (a,b,c p < 0.05). The addition of IGF-1 to the culture medium increased blastocyst rate in comparison to HS-control group. However, IGF-1 had more beneficial effects on ACR when added during both maturation and culture than during maturation or culture only. In conclusion, negative effects of HS on oocyte developmental competence can be reduced by adding IGF-1 during both maturation and culture.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
Oocyte maturation, Heat stress, IGF1, Apoptosis
in
REPRODUCTION IN DOMESTIC ANIMALS
Reprod. Domest. Anim.
editor
Laszlo Solti
volume
45
issue
suppl. 3
issue title
14th Annual conference of the European Society for Domestic Animal Reproduction (ESDAR)
article_number
P33
pages
71 - 71
conference name
14th Annual conference of the European Society for Domestic Animal Reproduction (ESDAR)
conference location
Eger, Hungary
conference start
2010-09-15
conference end
2010-09-18
JCR category
VETERINARY SCIENCES
JCR impact factor
1.606 (2010)
JCR rank
26/145 (2010)
JCR quartile
1 (2010)
ISSN
0936-6768
DOI
10.1111/j.1439-0531.2010.01654_3.x
language
English
UGent publication?
yes
classification
C3
id
1018505
handle
http://hdl.handle.net/1854/LU-1018505
date created
2010-08-05 15:39:56
date last changed
2010-08-06 08:50:35
@inproceedings{1018505,
  abstract     = {Heat stress (HS) reduces bovine oocyte developmental competence. Previous research showed that the addition of insulin like growth factor-1 (IGF-1) during maturation reduced apoptosis in heat stressed oocytes with no apparent effect on blastocyst rates. We hypothesized that adding IGF-1 during maturation and culture may enhance blastocyst rate and decrease apoptosis in blastocysts derived from heat stressed oocytes. During the first 12 h of maturation, HS (40.5\unmatched{0004}C) was applied while IGF-1 (100 ng/ml) was added during maturation (M), during culture (C) or during both (MC). Two control groups were included (negative-control: no HS nor IGF-1 and HS-control: HS but no IGF-1). Blastocyst rates were 27.9 {\textpm} 2.1a, 10.9 {\textpm} 1.2b, 9.7 {\textpm} 1.2b, 18.4 {\textpm} 2.4c, and 15.3 {\textpm} 1.9bc \% in negative-control, HS- ontrol, M, C and MC groups, respectively (a,b,c p {\textlangle} 0.05). Apoptotic cell ratios (ACR) were 9.7 {\textpm} 1a, 11.8 {\textpm} 1.2a, 11.6 {\textpm} 2a, 7.8 {\textpm} 0.8b and 5.9 {\textpm} 0.7c \% in negative-control, HS-control, M, C and MC groups, respectively (a,b,c p {\textlangle} 0.05). The addition of IGF-1 to the culture medium increased blastocyst rate in comparison to HS-control group. However, IGF-1 had more beneficial effects on ACR when added during both maturation and culture than during maturation or culture only. In conclusion, negative effects of HS on oocyte developmental competence can be reduced by adding IGF-1 during both maturation and culture.},
  articleno    = {P33},
  author       = {Shehab El Deen, Mohamed Ahmed MM and Leroy, Jo and Vandaele, Leen and Maes, Dominiek and Opsomer, Geert and Fadel, Moustafa and Van Soom, Ann},
  booktitle    = {REPRODUCTION IN DOMESTIC ANIMALS},
  editor       = {Solti, Laszlo},
  issn         = {0936-6768},
  keyword      = {Oocyte maturation,Heat stress,IGF1,Apoptosis},
  language     = {eng},
  location     = {Eger, Hungary},
  number       = {suppl. 3},
  pages        = {P33:71--P33:71},
  title        = {Addition of insulin like growth factor-1 during maturation and culture enhances blastocyst rate and reduces apoptosis in bovine blastocysts derived from heat stressed oocytes},
  url          = {http://dx.doi.org/10.1111/j.1439-0531.2010.01654\_3.x},
  volume       = {45},
  year         = {2010},
}

Chicago
Shehab El Deen, Mohamed Ahmed MM, Jo Leroy, Leen Vandaele, Dominiek Maes, Geert Opsomer, Moustafa Fadel, and Ann Van Soom. 2010. “Addition of Insulin Like Growth Factor-1 During Maturation and Culture Enhances Blastocyst Rate and Reduces Apoptosis in Bovine Blastocysts Derived from Heat Stressed Oocytes.” In Reproduction in Domestic Animals, ed. Laszlo Solti, 45:71–71.
APA
Shehab El Deen, M. A. M., Leroy, J., Vandaele, L., Maes, D., Opsomer, G., Fadel, M., & Van Soom, A. (2010). Addition of insulin like growth factor-1 during maturation and culture enhances blastocyst rate and reduces apoptosis in bovine blastocysts derived from heat stressed oocytes. In L. Solti (Ed.), REPRODUCTION IN DOMESTIC ANIMALS (Vol. 45, pp. 71–71). Presented at the 14th Annual conference of the European Society for Domestic Animal Reproduction (ESDAR).
Vancouver
1.
Shehab El Deen MAM, Leroy J, Vandaele L, Maes D, Opsomer G, Fadel M, et al. Addition of insulin like growth factor-1 during maturation and culture enhances blastocyst rate and reduces apoptosis in bovine blastocysts derived from heat stressed oocytes. In: Solti L, editor. REPRODUCTION IN DOMESTIC ANIMALS. 2010. p. 71–71.
MLA
Shehab El Deen, Mohamed Ahmed MM, Jo Leroy, Leen Vandaele, et al. “Addition of Insulin Like Growth Factor-1 During Maturation and Culture Enhances Blastocyst Rate and Reduces Apoptosis in Bovine Blastocysts Derived from Heat Stressed Oocytes.” Reproduction in Domestic Animals. Ed. Laszlo Solti. Vol. 45. 2010. 71–71. Print.