Ghent University Academic Bibliography

Advanced

Increased leaf size: different means to an end

Nathalie Gonzalez Sanchez UGent, Stefanie De Bodt UGent, Ronan Sulpice, Yusuke Jikumaru, Eunyoung Chae, Stijn Dhondt UGent, Twiggy Van Daele UGent, Liesbeth De Milde UGent, Detlef Weigel and Yuji Kamiya, et al. (2010) PLANT PHYSIOLOGY. 153(3). p.1261-1279
abstract
The final size of plant organs, such as leaves, is tightly controlled by environmental and genetic factors that must spatially and temporally coordinate cell expansion and cell cycle activity. However, this regulation of organ growth is still poorly understood. The aim of this study is to gain more insight into the genetic control of leaf size in Arabidopsis (Arabidopsis thaliana) by performing a comparative analysis of transgenic lines that produce enlarged leaves under standardized environmental conditions. To this end, we selected five genes belonging to different functional classes that all positively affect leaf size when overexpressed: AVP1, GRF5, JAW, BRI1, and GA20OX1. We show that the increase in leaf area in these lines depended on leaf position and growth conditions and that all five lines affected leaf size differently; however, in all cases, an increase in cell number was, entirely or predominantly, responsible for the leaf size enlargement. By analyzing hormone levels, transcriptome, and metabolome, we provide deeper insight into the molecular basis of the growth phenotype for the individual lines. A comparative analysis between these data sets indicates that enhanced organ growth is governed by different, seemingly independent pathways. The analysis of transgenic lines simultaneously overexpressing two growth-enhancing genes further supports the concept that multiple pathways independently converge on organ size control in Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GROWTH, CELL-PROLIFERATION, ORGAN SIZE, SYNOVIAL SARCOMA, PLANT DEVELOPMENT, ARABIDOPSIS-THALIANA, GENE, EXPRESSION, BRASSINOSTEROIDS, PYROPHOSPHORYLASE
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
153
issue
3
pages
1261 - 1279
Web of Science type
Article
Web of Science id
000279400200030
JCR category
PLANT SCIENCES
JCR impact factor
6.451 (2010)
JCR rank
8/185 (2010)
JCR quartile
1 (2010)
ISSN
0032-0889
DOI
10.1104/pp.110.156018
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1018456
handle
http://hdl.handle.net/1854/LU-1018456
date created
2010-08-05 15:22:30
date last changed
2012-09-19 14:03:48
@article{1018456,
  abstract     = {The final size of plant organs, such as leaves, is tightly controlled by environmental and genetic factors that must spatially and temporally coordinate cell expansion and cell cycle activity. However, this regulation of organ growth is still poorly understood. The aim of this study is to gain more insight into the genetic control of leaf size in Arabidopsis (Arabidopsis thaliana) by performing a comparative analysis of transgenic lines that produce enlarged leaves under standardized environmental conditions. To this end, we selected five genes belonging to different functional classes that all positively affect leaf size when overexpressed: AVP1, GRF5, JAW, BRI1, and GA20OX1. We show that the increase in leaf area in these lines depended on leaf position and growth conditions and that all five lines affected leaf size differently; however, in all cases, an increase in cell number was, entirely or predominantly, responsible for the leaf size enlargement. By analyzing hormone levels, transcriptome, and metabolome, we provide deeper insight into the molecular basis of the growth phenotype for the individual lines. A comparative analysis between these data sets indicates that enhanced organ growth is governed by different, seemingly independent pathways. The analysis of transgenic lines simultaneously overexpressing two growth-enhancing genes further supports the concept that multiple pathways independently converge on organ size control in Arabidopsis.},
  author       = {Gonzalez Sanchez, Nathalie and De Bodt, Stefanie and Sulpice, Ronan and Jikumaru, Yusuke and Chae, Eunyoung and Dhondt, Stijn and Van Daele, Twiggy and De Milde, Liesbeth and Weigel, Detlef and Kamiya, Yuji and Stitt, Mark and Beemster, Gerrit and Inz{\'e}, Dirk},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {GROWTH,CELL-PROLIFERATION,ORGAN SIZE,SYNOVIAL SARCOMA,PLANT DEVELOPMENT,ARABIDOPSIS-THALIANA,GENE,EXPRESSION,BRASSINOSTEROIDS,PYROPHOSPHORYLASE},
  language     = {eng},
  number       = {3},
  pages        = {1261--1279},
  title        = {Increased leaf size: different means to an end},
  url          = {http://dx.doi.org/10.1104/pp.110.156018},
  volume       = {153},
  year         = {2010},
}

Chicago
Gonzalez Sanchez, Nathalie, Stefanie De Bodt, Ronan Sulpice, Yusuke Jikumaru, Eunyoung Chae, Stijn Dhondt, Twiggy Van Daele, et al. 2010. “Increased Leaf Size: Different Means to an End.” Plant Physiology 153 (3): 1261–1279.
APA
Gonzalez Sanchez, N., De Bodt, S., Sulpice, R., Jikumaru, Y., Chae, E., Dhondt, S., Van Daele, T., et al. (2010). Increased leaf size: different means to an end. PLANT PHYSIOLOGY, 153(3), 1261–1279.
Vancouver
1.
Gonzalez Sanchez N, De Bodt S, Sulpice R, Jikumaru Y, Chae E, Dhondt S, et al. Increased leaf size: different means to an end. PLANT PHYSIOLOGY. 2010;153(3):1261–79.
MLA
Gonzalez Sanchez, Nathalie, Stefanie De Bodt, Ronan Sulpice, et al. “Increased Leaf Size: Different Means to an End.” PLANT PHYSIOLOGY 153.3 (2010): 1261–1279. Print.