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Mitochondrial genome analysis of the predatory mite Phytoseiulus persimilis and a revisit of the Metaseiulus occidentalis mitochondrial genome

Wannes Dermauw (UGent) , Bartel Vanholme (UGent) , Luc Tirry (UGent) and Thomas Van Leeuwen (UGent)
(2010) GENOME. 53(4). p.285-301
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Abstract
In this study we sequenced and analysed the complete mitochondrial (mt) genome of the Chilean predatory mite Phytoseiulus persimilis Athias-Henriot (Chelicerata: Acari: Mesostigmata: Phytoseiidae: Amblyseiinae). The 16 199 bp genome (79.8% AT) contains the standard set of 13 protein-coding and 24 RNA genes. Compared with the ancestral arthropod mtDNA pattern, the gene order is extremely reshuffled (35 genes changed position) and represents a novel arrangement within the arthropods. This is probably related to the presence of several large noncoding regions in the genome. In contrast with the mt genome of the closely related species Metaseiulus occidentalis (Phytoseiidae: Typhlodrominae) which was reported to be unusually large (24 961 bp), to lack nad6 and nad3 protein-coding genes, and to contain 22 tRNAs without T-arms the genome of P. persimilis has all the features of a standard metazoan mt genome. Consequently, we performed additional experiments on the M. occidentalis mt genome. Our preliminary restriction digests and Southern hybridization data revealed that this genome is smaller than previously reported. In addition, we cloned nad3 in M. occidentalis and positioned this gene between nad4L and 12S-rRNA on the mt genome. Finally, we report that at least 15 of the 22 tRNAs in the M. occidentalis mt genome can be folded into canonical cloverleaf structures similar to their counterparts in P. persimilis.
Keywords
ARRANGEMENT, SUBSTITUTION, SEQUENCE EVOLUTION, PARALLEL EVOLUTION, REARRANGEMENTS, TRANSFER-RNA GENES, CRAB LIMULUS-POLYPHEMUS, DROSOPHILA-MELANOGASTER, MODEL, DNA-SEQUENCE, tRNA, Phytoseiidae, duplication, control region, gene arrangement

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Citation

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Chicago
Dermauw, Wannes, Bartel Vanholme, Luc Tirry, and Thomas Van Leeuwen. 2010. “Mitochondrial Genome Analysis of the Predatory Mite Phytoseiulus Persimilis and a Revisit of the Metaseiulus Occidentalis Mitochondrial Genome.” Genome 53 (4): 285–301.
APA
Dermauw, W., Vanholme, B., Tirry, L., & Van Leeuwen, T. (2010). Mitochondrial genome analysis of the predatory mite Phytoseiulus persimilis and a revisit of the Metaseiulus occidentalis mitochondrial genome. GENOME, 53(4), 285–301.
Vancouver
1.
Dermauw W, Vanholme B, Tirry L, Van Leeuwen T. Mitochondrial genome analysis of the predatory mite Phytoseiulus persimilis and a revisit of the Metaseiulus occidentalis mitochondrial genome. GENOME. 2010;53(4):285–301.
MLA
Dermauw, Wannes, Bartel Vanholme, Luc Tirry, et al. “Mitochondrial Genome Analysis of the Predatory Mite Phytoseiulus Persimilis and a Revisit of the Metaseiulus Occidentalis Mitochondrial Genome.” GENOME 53.4 (2010): 285–301. Print.
@article{1016344,
  abstract     = {In this study we sequenced and analysed the complete mitochondrial (mt) genome of the Chilean predatory mite Phytoseiulus persimilis Athias-Henriot (Chelicerata: Acari: Mesostigmata: Phytoseiidae: Amblyseiinae). The 16 199 bp genome (79.8\% AT) contains the standard set of 13 protein-coding and 24 RNA genes. Compared with the ancestral arthropod mtDNA pattern, the gene order is extremely reshuffled (35 genes changed position) and represents a novel arrangement within the arthropods. This is probably related to the presence of several large noncoding regions in the genome. In contrast with the mt genome of the closely related species Metaseiulus occidentalis (Phytoseiidae: Typhlodrominae) which was reported to be unusually large (24 961 bp), to lack nad6 and nad3 protein-coding genes, and to contain 22 tRNAs without T-arms the genome of P. persimilis has all the features of a standard metazoan mt genome. Consequently, we performed additional experiments on the M. occidentalis mt genome. Our preliminary restriction digests and Southern hybridization data revealed that this genome is smaller than previously reported. In addition, we cloned nad3 in M. occidentalis and positioned this gene between nad4L and 12S-rRNA on the mt genome. Finally, we report that at least 15 of the 22 tRNAs in the M. occidentalis mt genome can be folded into canonical cloverleaf structures similar to their counterparts in P. persimilis.},
  author       = {Dermauw, Wannes and Vanholme, Bartel and Tirry, Luc and Van Leeuwen, Thomas},
  issn         = {0831-2796},
  journal      = {GENOME},
  keyword      = {ARRANGEMENT,SUBSTITUTION,SEQUENCE EVOLUTION,PARALLEL EVOLUTION,REARRANGEMENTS,TRANSFER-RNA GENES,CRAB LIMULUS-POLYPHEMUS,DROSOPHILA-MELANOGASTER,MODEL,DNA-SEQUENCE,tRNA,Phytoseiidae,duplication,control region,gene arrangement},
  language     = {eng},
  number       = {4},
  pages        = {285--301},
  title        = {Mitochondrial genome analysis of the predatory mite Phytoseiulus persimilis and a revisit of the Metaseiulus occidentalis mitochondrial genome},
  url          = {http://dx.doi.org/10.1139/G10-004},
  volume       = {53},
  year         = {2010},
}

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