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The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs

Antita Adriaens (UGent) , Simon Vermeire (UGent) , Tim Waelbers (UGent) , Luc Duchateau (UGent) , Stanislas Sys (UGent) , Sylvia Van Dorpe (UGent) , Jos Eersels, Bart De Spiegeleer (UGent) , Kathelijne Peremans (UGent) and Ingeborgh Polis (UGent)
Author
Organization
Abstract
The influence of systemic morphine on cerebral postsynaptic serotonin-2A receptor (5-HT2A) binding was investigated in dogs using Single Photon Emission Computed Tomography (SPECT) with 123I-5I-R91150, a 5-HT2A radioligand. The 5-HT2A binding was estimated with (M) and without (control) morphine pretreatment (0.5 mg kg-1 intravenously (IV), 30 minutes prior to radioligand injection) in eight 5-year-old female beagles. Scans were carried out with a triple head gamma camera 90 minutes after 123I-5I-R91150 injection (15.07 ± 2.69 MBq kg-1 IV). Dogs were premedicated with dexmedetomidine 65 minutes after radioligand injection, after which anesthesia was induced with propofol and maintained with isoflurane in oxygen. Semiquantification, with the cerebellum, a region void of 5-HT2A receptors, as a reference region, was performed to define the 5-HT2A receptor binding index (BI) (parameter for available receptor density) in the frontal, parietal, temporal and occipital cortex and the subcortical region. Data were analyzed by mixed-model ANOVA. Significance was set at p < 0.05. A significantly decreased 5-HT2A receptor BI was found in M for the right and left frontal cortices (respectively 1.41 ± 0.06 and 1.44 ± 0.08) compared to control (respectively 1.53 ± 0.10 and 1.55 ± 0.11) with p = 0.012 and 0.040 respectively. No significant differences were noted for the other regions. In conclusion, morphine administration decreases frontocortical 5-HT2A receptor availability. This confirms an interaction between the serotonergic and the opioid neurotransmitter system. Whether the decreased radioligand binding is the consequence of decreased receptor density due to direct internalization or the result of indirect actions, such as increased release of endogenous serotonin, remains to be elucidated. This study was supported by the Ghent University Special Research Fund (grant n°01J06109).

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MLA
Adriaens, Antita, et al. “The Influence of Morphine on 5-HT2A Receptor Availability Using SPECT: A Preliminary Study in Dogs.” Association of Veterinary Anaesthetists Spring Meeting, Abstracts, 2010.
APA
Adriaens, A., Vermeire, S., Waelbers, T., Duchateau, L., Sys, S., Van Dorpe, S., … Polis, I. (2010). The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs. Association of Veterinary Anaesthetists Spring Meeting, Abstracts. Presented at the Association of Veterinary Anaesthetists spring meeting, Cambridge, UK.
Chicago author-date
Adriaens, Antita, Simon Vermeire, Tim Waelbers, Luc Duchateau, Stanislas Sys, Sylvia Van Dorpe, Jos Eersels, Bart De Spiegeleer, Kathelijne Peremans, and Ingeborgh Polis. 2010. “The Influence of Morphine on 5-HT2A Receptor Availability Using SPECT: A Preliminary Study in Dogs.” In Association of Veterinary Anaesthetists Spring Meeting, Abstracts.
Chicago author-date (all authors)
Adriaens, Antita, Simon Vermeire, Tim Waelbers, Luc Duchateau, Stanislas Sys, Sylvia Van Dorpe, Jos Eersels, Bart De Spiegeleer, Kathelijne Peremans, and Ingeborgh Polis. 2010. “The Influence of Morphine on 5-HT2A Receptor Availability Using SPECT: A Preliminary Study in Dogs.” In Association of Veterinary Anaesthetists Spring Meeting, Abstracts.
Vancouver
1.
Adriaens A, Vermeire S, Waelbers T, Duchateau L, Sys S, Van Dorpe S, et al. The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs. In: Association of Veterinary Anaesthetists Spring Meeting, Abstracts. 2010.
IEEE
[1]
A. Adriaens et al., “The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs,” in Association of Veterinary Anaesthetists Spring Meeting, Abstracts, Cambridge, UK, 2010.
@inproceedings{1012600,
  abstract     = {{The influence of systemic morphine on cerebral postsynaptic serotonin-2A receptor (5-HT2A) binding was investigated in dogs using Single Photon Emission Computed Tomography (SPECT) with 123I-5I-R91150, a 5-HT2A radioligand. 
   The 5-HT2A binding was estimated with (M) and without (control) morphine pretreatment (0.5 mg kg-1 intravenously (IV), 30 minutes prior to radioligand injection) in eight 5-year-old female beagles. Scans were carried out with a triple head gamma camera 90 minutes after 123I-5I-R91150 injection (15.07 ± 2.69 MBq kg-1 IV). Dogs were premedicated with dexmedetomidine 65 minutes after radioligand injection, after which anesthesia was induced with propofol and maintained with isoflurane in oxygen. Semiquantification, with the cerebellum, a region void of 5-HT2A receptors, as a reference region, was performed to define the 5-HT2A receptor binding index (BI) (parameter for available receptor density) in the frontal, parietal, temporal and occipital cortex and the subcortical region. Data were analyzed by mixed-model ANOVA. Significance was set at p < 0.05.
   A significantly decreased 5-HT2A receptor BI was found in M for the right and left frontal cortices (respectively 1.41 ± 0.06 and 1.44 ± 0.08) compared to control (respectively 1.53 ± 0.10 and 1.55 ± 0.11) with p = 0.012 and 0.040 respectively. No significant differences were noted for the other regions.
   In conclusion, morphine administration decreases frontocortical 5-HT2A receptor availability. This confirms an interaction between the serotonergic and the opioid neurotransmitter system. Whether the decreased radioligand binding is the consequence of decreased receptor density due to direct internalization or the result of indirect actions, such as increased release of endogenous serotonin, remains to be elucidated.

   This study was supported by the Ghent University Special Research Fund (grant n°01J06109).}},
  author       = {{Adriaens, Antita and Vermeire, Simon and Waelbers, Tim and Duchateau, Luc and Sys, Stanislas and Van Dorpe, Sylvia and Eersels, Jos and De Spiegeleer, Bart and Peremans, Kathelijne and Polis, Ingeborgh}},
  booktitle    = {{Association of Veterinary Anaesthetists Spring Meeting, Abstracts}},
  language     = {{eng}},
  location     = {{Cambridge, UK}},
  title        = {{The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs}},
  year         = {{2010}},
}