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The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs

Antita Adriaens UGent, Simon Vermeire UGent, Tim Waelbers UGent, Luc Duchateau UGent, Stanislas Sys UGent, Sylvia Van Dorpe UGent, Jos Eersels, Bart De Spiegeleer UGent, Kathelijne Peremans UGent and Ingeborgh Polis UGent (2010)
abstract
The influence of systemic morphine on cerebral postsynaptic serotonin-2A receptor (5-HT2A) binding was investigated in dogs using Single Photon Emission Computed Tomography (SPECT) with 123I-5I-R91150, a 5-HT2A radioligand. The 5-HT2A binding was estimated with (M) and without (control) morphine pretreatment (0.5 mg kg-1 intravenously (IV), 30 minutes prior to radioligand injection) in eight 5-year-old female beagles. Scans were carried out with a triple head gamma camera 90 minutes after 123I-5I-R91150 injection (15.07 ± 2.69 MBq kg-1 IV). Dogs were premedicated with dexmedetomidine 65 minutes after radioligand injection, after which anesthesia was induced with propofol and maintained with isoflurane in oxygen. Semiquantification, with the cerebellum, a region void of 5-HT2A receptors, as a reference region, was performed to define the 5-HT2A receptor binding index (BI) (parameter for available receptor density) in the frontal, parietal, temporal and occipital cortex and the subcortical region. Data were analyzed by mixed-model ANOVA. Significance was set at p < 0.05. A significantly decreased 5-HT2A receptor BI was found in M for the right and left frontal cortices (respectively 1.41 ± 0.06 and 1.44 ± 0.08) compared to control (respectively 1.53 ± 0.10 and 1.55 ± 0.11) with p = 0.012 and 0.040 respectively. No significant differences were noted for the other regions. In conclusion, morphine administration decreases frontocortical 5-HT2A receptor availability. This confirms an interaction between the serotonergic and the opioid neurotransmitter system. Whether the decreased radioligand binding is the consequence of decreased receptor density due to direct internalization or the result of indirect actions, such as increased release of endogenous serotonin, remains to be elucidated. This study was supported by the Ghent University Special Research Fund (grant n°01J06109).
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
conference name
AVA Spring Meeting
conference location
Cambridge
conference start
2010-03-30
conference end
2010-03-31
language
English
UGent publication?
yes
classification
C3
id
1012600
handle
http://hdl.handle.net/1854/LU-1012600
date created
2010-07-15 13:14:39
date last changed
2010-07-16 09:23:17
@inproceedings{1012600,
  abstract     = {The influence of systemic morphine on cerebral postsynaptic serotonin-2A receptor (5-HT2A) binding was investigated in dogs using Single Photon Emission Computed Tomography (SPECT) with 123I-5I-R91150, a 5-HT2A radioligand. 
   The 5-HT2A binding was estimated with (M) and without (control) morphine pretreatment (0.5 mg kg-1 intravenously (IV), 30 minutes prior to radioligand injection) in eight 5-year-old female beagles. Scans were carried out with a triple head gamma camera 90 minutes after 123I-5I-R91150 injection (15.07 {\textpm} 2.69 MBq kg-1 IV). Dogs were premedicated with dexmedetomidine 65 minutes after radioligand injection, after which anesthesia was induced with propofol and maintained with isoflurane in oxygen. Semiquantification, with the cerebellum, a region void of 5-HT2A receptors, as a reference region, was performed to define the 5-HT2A receptor binding index (BI) (parameter for available receptor density) in the frontal, parietal, temporal and occipital cortex and the subcortical region. Data were analyzed by mixed-model ANOVA. Significance was set at p {\textlangle} 0.05.
   A significantly decreased 5-HT2A receptor BI was found in M for the right and left frontal cortices (respectively 1.41 {\textpm} 0.06 and 1.44 {\textpm} 0.08) compared to control (respectively 1.53 {\textpm} 0.10 and 1.55 {\textpm} 0.11) with p = 0.012 and 0.040 respectively. No significant differences were noted for the other regions.
   In conclusion, morphine administration decreases frontocortical 5-HT2A receptor availability. This confirms an interaction between the serotonergic and the opioid neurotransmitter system. Whether the decreased radioligand binding is the consequence of decreased receptor density due to direct internalization or the result of indirect actions, such as increased release of endogenous serotonin, remains to be elucidated.

   This study was supported by the Ghent University Special Research Fund (grant n{\textdegree}01J06109).},
  author       = {Adriaens, Antita and Vermeire, Simon and Waelbers, Tim and Duchateau, Luc and Sys, Stanislas and Van Dorpe, Sylvia and Eersels, Jos and De Spiegeleer, Bart and Peremans, Kathelijne and Polis, Ingeborgh},
  language     = {eng},
  location     = {Cambridge},
  title        = {The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs},
  year         = {2010},
}

Chicago
Adriaens, Antita, Simon Vermeire, Tim Waelbers, Luc Duchateau, Stanislas Sys, Sylvia Van Dorpe, Jos Eersels, Bart De Spiegeleer, Kathelijne Peremans, and Ingeborgh Polis. 2010. “The Influence of Morphine on 5-HT2A Receptor Availability Using SPECT: a Preliminary Study in Dogs.” In .
APA
Adriaens, Antita, Vermeire, S., Waelbers, T., Duchateau, L., Sys, S., Van Dorpe, S., Eersels, J., et al. (2010). The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs. Presented at the AVA Spring Meeting.
Vancouver
1.
Adriaens A, Vermeire S, Waelbers T, Duchateau L, Sys S, Van Dorpe S, et al. The influence of morphine on 5-HT2A receptor availability using SPECT: a preliminary study in dogs. 2010.
MLA
Adriaens, Antita, Simon Vermeire, Tim Waelbers, et al. “The Influence of Morphine on 5-HT2A Receptor Availability Using SPECT: a Preliminary Study in Dogs.” 2010. Print.