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Analytical evaluation of a rapid on-site oral fluid drug test

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Abstract
There is a need for a reliable rapid on-site oral fluid test that can be used in police controls to detect impaired drivers. We evaluated the Varian OralabA (R) 6 and collected two oral fluid samples from 250 subjects, one with the Varian OralabA (R) 6 and one with the StatSure (TM) SalivaaEuro cent Sampler (TM). The OralabA (R) 6 can detect six drug types: amphetamines, methamphetamine, cocaine, opiates, delta9-tetrahydrocannabinol (THC), and phencyclidine (PCP). On-site results were obtained within 10 to 15 min. The sample collected with StatSure (TM) was analyzed using liquid chromatography-tandem mass spectrometry after liquid-liquid extraction and these results were used as a reference to determine prevalence, sensitivity, and specificity. Two cut-off values were used in the evaluation. The Varian cut-off values were: amphetamine 50 ng/mL, cocaine 20 ng/mL, opiates 40 ng/mL, and THC 50 ng/mL. The DRUID cut-offs were: amphetamine 25 ng/mL, cocaine 20 ng/mL, opiates 20 ng/mL, and THC 1 ng/mL. Applying the first cut-offs, prevalence, sensitivity, and specificity were: amphetamine 10%, 76%, 100%; cocaine 23%, 34%, 100%; opiates 38%, 83%, 94%; and THC 18%, 41%, 99%. The DRUID cut-off values gave the following results: amphetamine 14%, 56%, 100%; cocaine 28%, 34%, 100%; opiates 49%, 68%, 98%, and THC 45%, 16%, 99%. The specificity of the OralabA (R) 6 is generally good. For both cut-offs, sensitivity was low for cocaine and THC. Therefore, the Varian OralabA (R) 6 test is not sensitive enough to be applied during roadside police controls.
Keywords
DRIVERS, DEVICES, Varian Oralab (R) 6, Sensitivity, Point-of-care test, Saliva

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Citation

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Chicago
Goessaert, An-Sofie, Kristof Pil, Jolien Veramme, and Alain Verstraete. 2010. “Analytical Evaluation of a Rapid On-site Oral Fluid Drug Test.” Analytical and Bioanalytical Chemistry 396 (7): 2461–2468.
APA
Goessaert, A.-S., Pil, K., Veramme, J., & Verstraete, A. (2010). Analytical evaluation of a rapid on-site oral fluid drug test. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 396(7), 2461–2468. Presented at the 47th Annual Meeting of the International-Association-of-Forensic-Toxicologists.
Vancouver
1.
Goessaert A-S, Pil K, Veramme J, Verstraete A. Analytical evaluation of a rapid on-site oral fluid drug test. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. HEIDELBERG: SPRINGER HEIDELBERG; 2010;396(7):2461–8.
MLA
Goessaert, An-Sofie et al. “Analytical Evaluation of a Rapid On-site Oral Fluid Drug Test.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 396.7 (2010): 2461–2468. Print.
@article{1008531,
  abstract     = {There is a need for a reliable rapid on-site oral fluid test that can be used in police controls to detect impaired drivers. We evaluated the Varian OralabA (R) 6 and collected two oral fluid samples from 250 subjects, one with the Varian OralabA (R) 6 and one with the StatSure (TM) SalivaaEuro cent Sampler (TM). The OralabA (R) 6 can detect six drug types: amphetamines, methamphetamine, cocaine, opiates, delta9-tetrahydrocannabinol (THC), and phencyclidine (PCP). On-site results were obtained within 10 to 15 min. The sample collected with StatSure (TM) was analyzed using liquid chromatography-tandem mass spectrometry after liquid-liquid extraction and these results were used as a reference to determine prevalence, sensitivity, and specificity. Two cut-off values were used in the evaluation. The Varian cut-off values were: amphetamine 50 ng/mL, cocaine 20 ng/mL, opiates 40 ng/mL, and THC 50 ng/mL. The DRUID cut-offs were: amphetamine 25 ng/mL, cocaine 20 ng/mL, opiates 20 ng/mL, and THC 1 ng/mL. Applying the first cut-offs, prevalence, sensitivity, and specificity were: amphetamine 10%, 76%, 100%; cocaine 23%, 34%, 100%; opiates 38%, 83%, 94%; and THC 18%, 41%, 99%. The DRUID cut-off values gave the following results: amphetamine 14%, 56%, 100%; cocaine 28%, 34%, 100%; opiates 49%, 68%, 98%, and THC 45%, 16%, 99%. The specificity of the OralabA (R) 6 is generally good. For both cut-offs, sensitivity was low for cocaine and THC. Therefore, the Varian OralabA (R) 6 test is not sensitive enough to be applied during roadside police controls.},
  author       = {Goessaert, An-Sofie and Pil, Kristof and Veramme, Jolien and Verstraete, Alain},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keywords     = {DRIVERS,DEVICES,Varian Oralab (R) 6,Sensitivity,Point-of-care test,Saliva},
  language     = {eng},
  location     = {Geneva, SWITZERLAND},
  number       = {7},
  pages        = {2461--2468},
  publisher    = {SPRINGER HEIDELBERG},
  title        = {Analytical evaluation of a rapid on-site oral fluid drug test},
  url          = {http://dx.doi.org/10.1007/s00216-010-3463-8},
  volume       = {396},
  year         = {2010},
}

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