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Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma

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Abstract
Background: Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors. Results: We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method on 52 neuroblastoma tumors showed that Multiplex Amplicon Quantification can reliably detect the important genomic aberrations. Conclusion: Multiplex Amplicon Quantification is a low-cost and high-throughput PCR-based technique that can reliably detect copy number alterations in regions with prognostic relevance for neuroblastoma.
Keywords
SUBGROUPS, ARRAY CGH, DEMENTIA

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Chicago
Kumps, Candy, Nadine Van Roy, L Heyrman, D Goossens, J Del-Favero, R Noguera, Jo Vandesompele, Franki Speleman, and Katleen De Preter. 2010. “Multiplex Amplicon Quantification (MAQ), a Fast and Efficient Method for the Simultaneous Detection of Copy Number Alterations in Neuroblastoma.” Bmc Genomics 11.
APA
Kumps, C., Van Roy, N., Heyrman, L., Goossens, D., Del-Favero, J., Noguera, R., Vandesompele, J., et al. (2010). Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma. BMC GENOMICS, 11.
Vancouver
1.
Kumps C, Van Roy N, Heyrman L, Goossens D, Del-Favero J, Noguera R, et al. Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma. BMC GENOMICS. LONDON: BIOMED CENTRAL LTD; 2010;11.
MLA
Kumps, Candy et al. “Multiplex Amplicon Quantification (MAQ), a Fast and Efficient Method for the Simultaneous Detection of Copy Number Alterations in Neuroblastoma.” BMC GENOMICS 11 (2010): n. pag. Print.
@article{1004298,
  abstract     = {Background: Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors.
Results: We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method on 52 neuroblastoma tumors showed that Multiplex Amplicon Quantification can reliably detect the important genomic aberrations.
Conclusion: Multiplex Amplicon Quantification is a low-cost and high-throughput PCR-based technique that can reliably detect copy number alterations in regions with prognostic relevance for neuroblastoma.},
  articleno    = {298},
  author       = {Kumps, Candy and Van Roy, Nadine and Heyrman, L and Goossens, D and Del-Favero, J and Noguera, R and Vandesompele, Jo and Speleman, Franki and De Preter, Katleen},
  issn         = {1471-2164},
  journal      = {BMC GENOMICS},
  keywords     = {SUBGROUPS,ARRAY CGH,DEMENTIA},
  language     = {eng},
  pages        = {8},
  publisher    = {BIOMED CENTRAL LTD},
  title        = {Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma},
  url          = {http://dx.doi.org/10.1186/1471-2164-11-298},
  volume       = {11},
  year         = {2010},
}

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