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Cholesterol and desmosterol in two sperm populations separated on Sil-Select gradient

A Zalata, A Hassan, Armand Christophe UGent, Frank Comhaire UGent and T Mostafa (2010) INTERNATIONAL JOURNAL OF ANDROLOGY. 33(3). p.528-535
abstract
P>Sperm lipids are important for sperm viability, maturity and function. This study aimed to identify cholesterol and desmosterol composition of human spermatozoa of two sperm populations separated on Sil-Select gradient. Forty-eight males were divided into four groups namely healthy men (n = 13), asthenozoospermia (n = 11), asthenoteratozoospermia (n = 10) and oligoasthenoteratozoospermia (n = 14). Sperm cholesterol and desmosterol were estimated in two human sperm population separated by centrifugation in a discontinuous Sil-Select gradient. The results showed that cholesterol and desmosterol were the major sterols in human spermatozoa. Spermatozoa recovered from upper/lower layer interface (fraction I) had low fertilization potential, while those from the base (fraction II) had high fertilization potential. Median values of cholesterol and desmosterol in fraction I were 2.55 mu mol and 0.77 mu mol/109 spermatozoa and in fraction II were 1.16 mu mol and 0.27 mu mol/109 spermatozoa. Cholesterol/desmosterol ratio was significantly higher in fraction II than I (4.8 vs. 3.2, p < 0.01). Cholesterol, desmosterol, total phospholipids and sterols/phospholipids were negatively correlated with sperm concentrations, sperm motility, linear velocity, normal sperm morphology and acrosome reaction percentage whereas cholesterol/desmosterol ratio was positively correlated with these parameters. It is concluded that the difference in sterol composition of sperm subpopulations separated on Sil-Select gradient suggests that composition of sterols is related to sperm functions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MALES, cholesterol, desmosterol, lipids, male infertility, semen, spermatozoa, FATTY-ACID-COMPOSITION, HUMAN SPERMATOZOA, DOCOSAHEXAENOIC ACID, MEMBRANE CHOLESTEROL, PLASMA-MEMBRANE, CAPACITATION, SEMEN, EFFLUX, PHOSPHOLIPIDS
journal title
INTERNATIONAL JOURNAL OF ANDROLOGY
Int. J. Androl.
volume
33
issue
3
pages
528 - 535
Web of Science type
article
Web of Science id
000277288100007
JCR category
ANDROLOGY
JCR impact factor
3.601 (2010)
JCR rank
1/5 (2010)
JCR quartile
1 (2010)
ISSN
0105-6263
DOI
10.1111/j.1365-2605.2009.00961.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1001931
handle
http://hdl.handle.net/1854/LU-1001931
date created
2010-07-02 16:56:22
date last changed
2010-09-23 14:09:47
@article{1001931,
  abstract     = {P{\textrangle}Sperm lipids are important for sperm viability, maturity and function. This study aimed to identify cholesterol and desmosterol composition of human spermatozoa of two sperm populations separated on Sil-Select gradient. Forty-eight males were divided into four groups namely healthy men (n = 13), asthenozoospermia (n = 11), asthenoteratozoospermia (n = 10) and oligoasthenoteratozoospermia (n = 14). Sperm cholesterol and desmosterol were estimated in two human sperm population separated by centrifugation in a discontinuous Sil-Select gradient. The results showed that cholesterol and desmosterol were the major sterols in human spermatozoa. Spermatozoa recovered from upper/lower layer interface (fraction I) had low fertilization potential, while those from the base (fraction II) had high fertilization potential. Median values of cholesterol and desmosterol in fraction I were 2.55 mu mol and 0.77 mu mol/109 spermatozoa and in fraction II were 1.16 mu mol and 0.27 mu mol/109 spermatozoa. Cholesterol/desmosterol ratio was significantly higher in fraction II than I (4.8 vs. 3.2, p {\textlangle} 0.01). Cholesterol, desmosterol, total phospholipids and sterols/phospholipids were negatively correlated with sperm concentrations, sperm motility, linear velocity, normal sperm morphology and acrosome reaction percentage whereas cholesterol/desmosterol ratio was positively correlated with these parameters. It is concluded that the difference in sterol composition of sperm subpopulations separated on Sil-Select gradient suggests that composition of sterols is related to sperm functions.},
  author       = {Zalata, A and Hassan, A and Christophe, Armand and Comhaire, Frank and Mostafa, T},
  issn         = {0105-6263},
  journal      = {INTERNATIONAL JOURNAL OF ANDROLOGY},
  keyword      = {MALES,cholesterol,desmosterol,lipids,male infertility,semen,spermatozoa,FATTY-ACID-COMPOSITION,HUMAN SPERMATOZOA,DOCOSAHEXAENOIC ACID,MEMBRANE CHOLESTEROL,PLASMA-MEMBRANE,CAPACITATION,SEMEN,EFFLUX,PHOSPHOLIPIDS},
  language     = {eng},
  number       = {3},
  pages        = {528--535},
  title        = {Cholesterol and desmosterol in two sperm populations separated on Sil-Select gradient},
  url          = {http://dx.doi.org/10.1111/j.1365-2605.2009.00961.x},
  volume       = {33},
  year         = {2010},
}

Chicago
Zalata, A, A Hassan, Armand Christophe, Frank Comhaire, and T Mostafa. 2010. “Cholesterol and Desmosterol in Two Sperm Populations Separated on Sil-Select Gradient.” International Journal of Andrology 33 (3): 528–535.
APA
Zalata, A., Hassan, A., Christophe, A., Comhaire, F., & Mostafa, T. (2010). Cholesterol and desmosterol in two sperm populations separated on Sil-Select gradient. INTERNATIONAL JOURNAL OF ANDROLOGY, 33(3), 528–535.
Vancouver
1.
Zalata A, Hassan A, Christophe A, Comhaire F, Mostafa T. Cholesterol and desmosterol in two sperm populations separated on Sil-Select gradient. INTERNATIONAL JOURNAL OF ANDROLOGY. 2010;33(3):528–35.
MLA
Zalata, A, A Hassan, Armand Christophe, et al. “Cholesterol and Desmosterol in Two Sperm Populations Separated on Sil-Select Gradient.” INTERNATIONAL JOURNAL OF ANDROLOGY 33.3 (2010): 528–535. Print.