@article{01KHYFNVP2AHDH2ERD4YSKQRJ9,
  abstract     = {{Melia volkensii is a drought-tolerant hardwood of high ecological and economic value in arid and semi-arid Africa, but conventional propagation is limited by poor seed viability and recalcitrance to regeneration. To explore molecular mechanisms of rejuvenation, we investigated telomere length and global DNA methylation in tissues derived from different propagation routes: adult micropropagation, juvenile seedlings, somatic embryogenesis, adventitious shoots, and adventitious roots. Telomere length, measured by Southern hybridization, varied significantly among tissue types. Adult micropropagated tissues showed the shortest telomeres (mean 5.0 kb), while juvenile seedlings (8.5 kb) and adventitious roots (8.9 kb) had markedly longer telomeres, suggesting a juvenile-like state. Somatic embryos (7.4 kb) and adventitious shoots (7.8 kb) exhibited intermediate lengths, indicating partial rejuvenation during regeneration. Global DNA methylation, quantified by ELISA, further distinguished somatic embryos, which showed the lowest absorbance (~ 2.0) and fold change (~ 0.88), significantly reduced compared to juvenile (~ 0.96), adventitious (~ 0.96), and adult tissues (~ 1.0). These findings demonstrate a strong link between telomere elongation, reduced 5-methylcytosine levels, and cellular rejuvenation in M. volkensii, offering valuable insights for optimizing clonal propagation and ensuring long-term genetic stability in forestry applications. Together, our results demonstrate that somatic embryogenesis induces molecular rejuvenation in Melia volkensii, characterized by coordinated telomeric elongation and epigenetic resetting, thereby establishing a mechanistic link between regeneration pathway and biological age in a non-model woody species.}},
  articleno    = {{54}},
  author       = {{Bhogar Suresh, Nandini and Aronen, Tuija and Magomere, Titus and Werbrouck, Stefaan}},
  issn         = {{0167-6857}},
  journal      = {{PLANT CELL TISSUE AND ORGAN CULTURE}},
  keywords     = {{DNA methylation,In vitro rejuvenation,Micropropagation,Melia volkensii,Somatic embryogenesis,Telomere length,DNA METHYLATION,PHASE-CHANGE,MICROPROPAGATION,SHORTEN,STRESS,TREES}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{10}},
  title        = {{Rejuvenation through somatic embryogenesis : epigenetic and telomeric reset in Melia volkensii}},
  url          = {{http://doi.org/10.1007/s11240-026-03382-6}},
  volume       = {{164}},
  year         = {{2026}},
}

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