Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana
(2026)
- Author
- Michael J Holdsworth, Charlene Dambire, Isabel Manrique Gil, Jorge Vicente, Kris Gevaert (UGent) , Frank Van Breusegem (UGent) and Neil Oldham
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- Abstract
- The N-degron pathways of ubiquitin mediated proteolysis target proteins for degradation dependent on the amino terminal residue, often produced after endopeptidase activity. Very few substrates have been identified in plants even though enzymes of these pathways are highly conserved in eukaryotes. Here we identify ELONGATED HYPOCOTYL5 (HY5), a master transcriptional regulator involved in many aspects of plant development, as a target for the endopeptidase METACASPASE (MC)9, producing the carboxy-terminal protein fragment (proteoform) E59-HY5. E59-HY5 is shown to be a substrate of the arginyl transferase (ATE) N-degron pathway, and influences physiological processes known to be controlled by HY5, including photomorphogenesis and the unfolded protein response. Conditional stability of E59-HY5 was shown to result from environmentally controlled ATE function, which may highlight a general mechanism for N-degron pathway regulation of proteoform and proteome function during growth and development.
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01KH8JBN432NR5TR4C1JD8RQWX
- MLA
- Holdsworth, Michael J., et al. Conditional Stability of HY5 through the ATE N-Degron Pathway Regulates Environmental Responses in Arabidopsis Thaliana. openRxiv, 2026, doi:10.64898/2026.02.10.705009.
- APA
- Holdsworth, M. J., Dambire, C., Manrique Gil, I., Vicente, J., Gevaert, K., Van Breusegem, F., & Oldham, N. (2026). Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana. https://doi.org/10.64898/2026.02.10.705009
- Chicago author-date
- Holdsworth, Michael J, Charlene Dambire, Isabel Manrique Gil, Jorge Vicente, Kris Gevaert, Frank Van Breusegem, and Neil Oldham. 2026. “Conditional Stability of HY5 through the ATE N-Degron Pathway Regulates Environmental Responses in Arabidopsis Thaliana.” openRxiv. https://doi.org/10.64898/2026.02.10.705009.
- Chicago author-date (all authors)
- Holdsworth, Michael J, Charlene Dambire, Isabel Manrique Gil, Jorge Vicente, Kris Gevaert, Frank Van Breusegem, and Neil Oldham. 2026. “Conditional Stability of HY5 through the ATE N-Degron Pathway Regulates Environmental Responses in Arabidopsis Thaliana.” openRxiv. doi:10.64898/2026.02.10.705009.
- Vancouver
- 1.Holdsworth MJ, Dambire C, Manrique Gil I, Vicente J, Gevaert K, Van Breusegem F, et al. Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana. openRxiv; 2026.
- IEEE
- [1]M. J. Holdsworth et al., “Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana.” openRxiv, 2026.
@misc{01KH8JBN432NR5TR4C1JD8RQWX,
abstract = {{The N-degron pathways of ubiquitin mediated proteolysis target proteins for degradation dependent on the amino terminal residue, often produced after endopeptidase activity. Very few substrates have been identified in plants even though enzymes of these pathways are highly conserved in eukaryotes. Here we identify ELONGATED HYPOCOTYL5 (HY5), a master transcriptional regulator involved in many aspects of plant development, as a target for the endopeptidase METACASPASE (MC)9, producing the carboxy-terminal protein fragment (proteoform) E59-HY5. E59-HY5 is shown to be a substrate of the arginyl transferase (ATE) N-degron pathway, and influences physiological processes known to be controlled by HY5, including photomorphogenesis and the unfolded protein response. Conditional stability of E59-HY5 was shown to result from environmentally controlled ATE function, which may highlight a general mechanism for N-degron pathway regulation of proteoform and proteome function during growth and development.}},
author = {{Holdsworth, Michael J and Dambire, Charlene and Manrique Gil, Isabel and Vicente, Jorge and Gevaert, Kris and Van Breusegem, Frank and Oldham, Neil}},
language = {{und}},
publisher = {{openRxiv}},
title = {{Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana}},
url = {{http://doi.org/10.64898/2026.02.10.705009}},
year = {{2026}},
}
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