The nuclear effector RIRG190 interacts with SAS10 to regulate arbuscular mycorrhizal symbiosis
- Author
- María Victoria Aparicio Chacón, Annick De Keyser (UGent) , Naomi Stuer (UGent) , Toon Leroy (UGent) , Evi Ceulemans, Juan Antonio López-Ráez, Alain Goossens (UGent) , Judith Van Dingenen (UGent) and Sofie Goormachtig (UGent)
- Organization
- Project
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- Understanding biotic stresses in plant roots through molecular interactomics in tomato
- Protein interplay during the establishment of arbuscular mycorrhiza: revealing the function of tomato root proteins interacting with fungal plant effectors
- Sugar Regulation of Nodulation: positioning the role of master sugar regulators during nodulation in soybean
- Improving the industrial and agricultural potential of arbuscular mycorrhiza through the modulation of the carbon status in tomato.
- Identification of in planta secreted fungal effectors and characterization of their interacting tomato proteins to ameliorate arbuscular mycorrhiza-based agriculture
- Abstract
- Most land plants engage in a mutualistic interaction with arbuscular mycorrhizal fungi (AMF), for which Rhizophagus irregularis is a model species. Like plant pathogenic fungi, AMF genomes encode hundreds of putative effector proteins. However, for only a few, the molecular mechanisms by which they alter the host's physiology are known. Here, we combined several reverse genetic approaches to unravel the role of the RIRG190 effector protein in arbuscular mycorrhiza (AM) symbiosis. Using multiple heterologous tools, evidence is provided that the RIRG190 effector is secreted and localizes to the plant nucleus. Moreover, by means of yeast two-hybrid (Y2H) and ratiometric bimolecular fluorescence complementation (rBIFC) assays, the data demonstrate that RIRG190 interacts with the protein Something About Silencing (SAS10), known to be involved in rRNA biogenesis in the nucleolus of cortical cells. Our findings suggest that rRNA biogenesis is a key process modulated by AMF, potentially to enhance plant metabolic activity, facilitating cell cycle progression, and to support the establishment of the symbiosis.
- Keywords
- AM symbiosis, Arabidopsis thaliana, Rhizophagus irregularis, Solanum lycopersicum, effector protein, nucleus, rRNA biogenesis, CELL-CYCLE, MEDICAGO-TRUNCATULA, PROTEIN, IDENTIFICATION, EXPRESSION, ROOTS, ESTABLISHMENT, COLONIZATION, ACTIVATION, REVEALS
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Aparicio Chacón et al (2025) International Journal of Molecular Sciences 26, 12178.pdf
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01KDZ19NY94J2S4DV7V6R8JMR1
- MLA
- Aparicio Chacón, María Victoria, et al. “The Nuclear Effector RIRG190 Interacts with SAS10 to Regulate Arbuscular Mycorrhizal Symbiosis.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 26, no. 24, 2025, doi:10.3390/ijms262412178.
- APA
- Aparicio Chacón, M. V., De Keyser, A., Stuer, N., Leroy, T., Ceulemans, E., López-Ráez, J. A., … Goormachtig, S. (2025). The nuclear effector RIRG190 interacts with SAS10 to regulate arbuscular mycorrhizal symbiosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 26(24). https://doi.org/10.3390/ijms262412178
- Chicago author-date
- Aparicio Chacón, María Victoria, Annick De Keyser, Naomi Stuer, Toon Leroy, Evi Ceulemans, Juan Antonio López-Ráez, Alain Goossens, Judith Van Dingenen, and Sofie Goormachtig. 2025. “The Nuclear Effector RIRG190 Interacts with SAS10 to Regulate Arbuscular Mycorrhizal Symbiosis.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 (24). https://doi.org/10.3390/ijms262412178.
- Chicago author-date (all authors)
- Aparicio Chacón, María Victoria, Annick De Keyser, Naomi Stuer, Toon Leroy, Evi Ceulemans, Juan Antonio López-Ráez, Alain Goossens, Judith Van Dingenen, and Sofie Goormachtig. 2025. “The Nuclear Effector RIRG190 Interacts with SAS10 to Regulate Arbuscular Mycorrhizal Symbiosis.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 (24). doi:10.3390/ijms262412178.
- Vancouver
- 1.Aparicio Chacón MV, De Keyser A, Stuer N, Leroy T, Ceulemans E, López-Ráez JA, et al. The nuclear effector RIRG190 interacts with SAS10 to regulate arbuscular mycorrhizal symbiosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2025;26(24).
- IEEE
- [1]M. V. Aparicio Chacón et al., “The nuclear effector RIRG190 interacts with SAS10 to regulate arbuscular mycorrhizal symbiosis,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 26, no. 24, 2025.
@article{01KDZ19NY94J2S4DV7V6R8JMR1,
abstract = {{Most land plants engage in a mutualistic interaction with arbuscular mycorrhizal fungi (AMF), for which Rhizophagus irregularis is a model species. Like plant pathogenic fungi, AMF genomes encode hundreds of putative effector proteins. However, for only a few, the molecular mechanisms by which they alter the host's physiology are known. Here, we combined several reverse genetic approaches to unravel the role of the RIRG190 effector protein in arbuscular mycorrhiza (AM) symbiosis. Using multiple heterologous tools, evidence is provided that the RIRG190 effector is secreted and localizes to the plant nucleus. Moreover, by means of yeast two-hybrid (Y2H) and ratiometric bimolecular fluorescence complementation (rBIFC) assays, the data demonstrate that RIRG190 interacts with the protein Something About Silencing (SAS10), known to be involved in rRNA biogenesis in the nucleolus of cortical cells. Our findings suggest that rRNA biogenesis is a key process modulated by AMF, potentially to enhance plant metabolic activity, facilitating cell cycle progression, and to support the establishment of the symbiosis.}},
articleno = {{12178}},
author = {{Aparicio Chacón, María Victoria and De Keyser, Annick and Stuer, Naomi and Leroy, Toon and Ceulemans, Evi and López-Ráez, Juan Antonio and Goossens, Alain and Van Dingenen, Judith and Goormachtig, Sofie}},
issn = {{1661-6596}},
journal = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
keywords = {{AM symbiosis,Arabidopsis thaliana,Rhizophagus irregularis,Solanum lycopersicum,effector protein,nucleus,rRNA biogenesis,CELL-CYCLE,MEDICAGO-TRUNCATULA,PROTEIN,IDENTIFICATION,EXPRESSION,ROOTS,ESTABLISHMENT,COLONIZATION,ACTIVATION,REVEALS}},
language = {{eng}},
number = {{24}},
pages = {{28}},
title = {{The nuclear effector RIRG190 interacts with SAS10 to regulate arbuscular mycorrhizal symbiosis}},
url = {{http://doi.org/10.3390/ijms262412178}},
volume = {{26}},
year = {{2025}},
}
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