Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars
- Author
- Stijn Van den Brande, Marijke Gijbels, Niels Wynant, Paulien Peeters, Yannick Gansemans (UGent) , Filip Van Nieuwerburgh (UGent) , Dulce Santos and Jozef Vanden Broeck
- Organization
- Abstract
- Since the discovery of the first microRNA (miRNA) in the nematode Caenorhabditis elegans, numerous novel miR-NAs have been identified which can regulate presumably every biological process in a wide range of metazoan species. In accordance, several insect miRNAs have been identified and functionally characterized. While regu-latory RNA pathways are traditionally described at an intracellular level, studies reporting on the presence and potential role of extracellular (small) sRNAs have been emerging in the last decade, mainly in mammalian sys-tems. Interestingly, evidence in several species indicates the functional transfer of extracellular RNAs between donor and recipient cells, illustrating RNA-based intercellular communication. In insects, however, reports on extracellular small RNAs are emerging but the number of detailed studies is still very limited. Here, we demon-strate the presence of stable sRNAs in the hemolymph of the migratory locust, Locusta migratoria. Moreover, the levels of several extracellular miRNAs (ex-miRNAs) present in locust hemolymph differed significantly between young and old fifth nymphal instars. In addition, we performed a 'proof of principle' experiment which suggested that extracellularly delivered miRNA molecules are capable of affecting the locusts' development.
- Keywords
- Agomir, Ecdysteroid, Extracellular small RNA, Insect, Moult, DOUBLE-STRANDED-RNA, BANTAM MIRNA, CELL-PROLIFERATION, GENE-EXPRESSION, DROSOPHILA, INTERFERENCE, MECHANISM, INSECT, METAMORPHOSIS, REPRESSION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01K33XM30MMR3D1VH1555T4D5J
- MLA
- Brande, Stijn Van den, et al. “Identification and Profiling of Stable MicroRNAs in Hemolymph of Young and Old Locusta Migratoria Fifth Instars.” CURRENT RESEARCH IN INSECT SCIENCE, vol. 2, 2022, doi:10.1016/j.cris.2022.100041.
- APA
- Brande, S. V. den, Gijbels, M., Wynant, N., Peeters, P., Gansemans, Y., Van Nieuwerburgh, F., … Vanden Broeck, J. (2022). Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars. CURRENT RESEARCH IN INSECT SCIENCE, 2. https://doi.org/10.1016/j.cris.2022.100041
- Chicago author-date
- Brande, Stijn Van den, Marijke Gijbels, Niels Wynant, Paulien Peeters, Yannick Gansemans, Filip Van Nieuwerburgh, Dulce Santos, and Jozef Vanden Broeck. 2022. “Identification and Profiling of Stable MicroRNAs in Hemolymph of Young and Old Locusta Migratoria Fifth Instars.” CURRENT RESEARCH IN INSECT SCIENCE 2. https://doi.org/10.1016/j.cris.2022.100041.
- Chicago author-date (all authors)
- Brande, Stijn Van den, Marijke Gijbels, Niels Wynant, Paulien Peeters, Yannick Gansemans, Filip Van Nieuwerburgh, Dulce Santos, and Jozef Vanden Broeck. 2022. “Identification and Profiling of Stable MicroRNAs in Hemolymph of Young and Old Locusta Migratoria Fifth Instars.” CURRENT RESEARCH IN INSECT SCIENCE 2. doi:10.1016/j.cris.2022.100041.
- Vancouver
- 1.Brande SV den, Gijbels M, Wynant N, Peeters P, Gansemans Y, Van Nieuwerburgh F, et al. Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars. CURRENT RESEARCH IN INSECT SCIENCE. 2022;2.
- IEEE
- [1]S. V. den Brande et al., “Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars,” CURRENT RESEARCH IN INSECT SCIENCE, vol. 2, 2022.
@article{01K33XM30MMR3D1VH1555T4D5J,
abstract = {{Since the discovery of the first microRNA (miRNA) in the nematode Caenorhabditis elegans, numerous novel miR-NAs have been identified which can regulate presumably every biological process in a wide range of metazoan species. In accordance, several insect miRNAs have been identified and functionally characterized. While regu-latory RNA pathways are traditionally described at an intracellular level, studies reporting on the presence and potential role of extracellular (small) sRNAs have been emerging in the last decade, mainly in mammalian sys-tems. Interestingly, evidence in several species indicates the functional transfer of extracellular RNAs between donor and recipient cells, illustrating RNA-based intercellular communication. In insects, however, reports on extracellular small RNAs are emerging but the number of detailed studies is still very limited. Here, we demon-strate the presence of stable sRNAs in the hemolymph of the migratory locust, Locusta migratoria. Moreover, the levels of several extracellular miRNAs (ex-miRNAs) present in locust hemolymph differed significantly between young and old fifth nymphal instars. In addition, we performed a 'proof of principle' experiment which suggested that extracellularly delivered miRNA molecules are capable of affecting the locusts' development.}},
articleno = {{100041}},
author = {{Brande, Stijn Van den and Gijbels, Marijke and Wynant, Niels and Peeters, Paulien and Gansemans, Yannick and Van Nieuwerburgh, Filip and Santos, Dulce and Vanden Broeck, Jozef}},
issn = {{2666-5158}},
journal = {{CURRENT RESEARCH IN INSECT SCIENCE}},
keywords = {{Agomir,Ecdysteroid,Extracellular small RNA,Insect,Moult,DOUBLE-STRANDED-RNA,BANTAM MIRNA,CELL-PROLIFERATION,GENE-EXPRESSION,DROSOPHILA,INTERFERENCE,MECHANISM,INSECT,METAMORPHOSIS,REPRESSION}},
language = {{eng}},
pages = {{11}},
title = {{Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars}},
url = {{http://doi.org/10.1016/j.cris.2022.100041}},
volume = {{2}},
year = {{2022}},
}
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