Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis
- Author
- Guillem Sanchez Sanchez, Maria Papadopoulou, Abdulkader Azouz, Yohannes Tafesse, Archita Mishra, Jerry K. Y. Chan, Yiping Fan, Isoline Verdebout, Silvana Porco, Frédérick Libert, Florent Ginhoux, Bart Vandekerckhove (UGent) , Stanislas Goriely and David Vermijlen
- Organization
- Abstract
- Developmental thymic waves of innate-like and adaptive-like gamma delta T cells have been described, but the current understanding of gamma delta T cell development is mainly limited to mouse models. Here, we combine single cell (sc) RNA gene expression and sc gamma delta T cell receptor (TCR) sequencing on fetal and pediatric gamma delta thymocytes in order to understand the ontogeny of human gamma delta T cells. Mature fetal gamma delta thymocytes (both the V gamma 9V delta 2 and nonV gamma 9V delta 2 subsets) are committed to either a type 1, a type 3 or a type 2-like effector fate displaying a wave-like pattern depending on gestation age, and are enriched for public CDR3 features upon maturation. Strikingly, these effector modules express different CDR3 sequences and follow distinct developmental trajectories. In contrast, the pediatric thymus generates only a small effector subset that is highly biased towards V gamma 9V delta 2 TCR usage and shows a mixed type 1/type 3 effector profile. Thus, our combined dataset of gene expression and detailed TCR information at the single-cell level identifies distinct functional thymic programming of gamma delta T cell immunity in human.
- Keywords
- T-CELLS, MICROBIAL EXPOSURE, DIFFERENTIATION, RECOGNITION, MATURATION, SUBSET, ALPHA, INTERLEUKIN-17, COSTIMULATION, HOMEOSTASIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JXF3JAW120CZXSM69X5V9216
- MLA
- Sanchez Sanchez, Guillem, et al. “Identification of Distinct Functional Thymic Programming of Fetal and Pediatric Human Γδ Thymocytes via Single-Cell Analysis.” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022, doi:10.1038/s41467-022-33488-2.
- APA
- Sanchez Sanchez, G., Papadopoulou, M., Azouz, A., Tafesse, Y., Mishra, A., Chan, J. K. Y., … Vermijlen, D. (2022). Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis. NATURE COMMUNICATIONS, 13(1). https://doi.org/10.1038/s41467-022-33488-2
- Chicago author-date
- Sanchez Sanchez, Guillem, Maria Papadopoulou, Abdulkader Azouz, Yohannes Tafesse, Archita Mishra, Jerry K. Y. Chan, Yiping Fan, et al. 2022. “Identification of Distinct Functional Thymic Programming of Fetal and Pediatric Human Γδ Thymocytes via Single-Cell Analysis.” NATURE COMMUNICATIONS 13 (1). https://doi.org/10.1038/s41467-022-33488-2.
- Chicago author-date (all authors)
- Sanchez Sanchez, Guillem, Maria Papadopoulou, Abdulkader Azouz, Yohannes Tafesse, Archita Mishra, Jerry K. Y. Chan, Yiping Fan, Isoline Verdebout, Silvana Porco, Frédérick Libert, Florent Ginhoux, Bart Vandekerckhove, Stanislas Goriely, and David Vermijlen. 2022. “Identification of Distinct Functional Thymic Programming of Fetal and Pediatric Human Γδ Thymocytes via Single-Cell Analysis.” NATURE COMMUNICATIONS 13 (1). doi:10.1038/s41467-022-33488-2.
- Vancouver
- 1.Sanchez Sanchez G, Papadopoulou M, Azouz A, Tafesse Y, Mishra A, Chan JKY, et al. Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis. NATURE COMMUNICATIONS. 2022;13(1).
- IEEE
- [1]G. Sanchez Sanchez et al., “Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis,” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022.
@article{01JXF3JAW120CZXSM69X5V9216,
abstract = {{Developmental thymic waves of innate-like and adaptive-like gamma delta T cells have been described, but the current understanding of gamma delta T cell development is mainly limited to mouse models. Here, we combine single cell (sc) RNA gene expression and sc gamma delta T cell receptor (TCR) sequencing on fetal and pediatric gamma delta thymocytes in order to understand the ontogeny of human gamma delta T cells. Mature fetal gamma delta thymocytes (both the V gamma 9V delta 2 and nonV gamma 9V delta 2 subsets) are committed to either a type 1, a type 3 or a type 2-like effector fate displaying a wave-like pattern depending on gestation age, and are enriched for public CDR3 features upon maturation. Strikingly, these effector modules express different CDR3 sequences and follow distinct developmental trajectories. In contrast, the pediatric thymus generates only a small effector subset that is highly biased towards V gamma 9V delta 2 TCR usage and shows a mixed type 1/type 3 effector profile. Thus, our combined dataset of gene expression and detailed TCR information at the single-cell level identifies distinct functional thymic programming of gamma delta T cell immunity in human.}},
articleno = {{5842}},
author = {{Sanchez Sanchez, Guillem and Papadopoulou, Maria and Azouz, Abdulkader and Tafesse, Yohannes and Mishra, Archita and Chan, Jerry K. Y. and Fan, Yiping and Verdebout, Isoline and Porco, Silvana and Libert, Frédérick and Ginhoux, Florent and Vandekerckhove, Bart and Goriely, Stanislas and Vermijlen, David}},
issn = {{2041-1723}},
journal = {{NATURE COMMUNICATIONS}},
keywords = {{T-CELLS,MICROBIAL EXPOSURE,DIFFERENTIATION,RECOGNITION,MATURATION,SUBSET,ALPHA,INTERLEUKIN-17,COSTIMULATION,HOMEOSTASIS}},
language = {{eng}},
number = {{1}},
pages = {{19}},
title = {{Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis}},
url = {{http://doi.org/10.1038/s41467-022-33488-2}},
volume = {{13}},
year = {{2022}},
}
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