- Author
- Sofie Rennen (UGent) , Victor Bosteels, Clint De Nolf (UGent) , Greta Webb (UGent) , Karo Van Lil (UGent) , Sandra Maréchal (UGent) , Jessica Vetters (UGent) , Evelien Van De Velde (UGent) , Farzaneh Fayazpour (UGent) , Ria Roelandt (UGent) , Niels Vandamme (UGent) , Kevin Verstaen (UGent) , Stefaan De Smedt (UGent) , Karine Breckpot, Bruno De Geest (UGent) , Rein Verbeke (UGent) , Ine Lentacker (UGent) and Sophie Janssens (UGent)
- Organization
- Project
-
- A novel role for the IRE1/XBP1 branch in dendritic cells: a signaling cascade matures
- Dissecting the molecular signals that drive immunogenic versus homeostatic maturation in dendritic cells. An old question revisited.
- LNP-DECODE: Deciphering the adjuvants activity of lipid nanoparticle-based mRNA vaccines in primary dendritic cells
- Decoding tumor antigens: dendritic cell maturation within the tumor environment
- The IRE1/XBP1 signaling axis in dendritic cell-mediated anti-tumor immune responses
- A novel role for the IRE1/XBP1 branch in dendritic cells: a signaling cascade matures.
- Abstract
- Dendritic cells (DCs) are short-lived immune cells that continuously roam our body in search for foreign or self-antigens. Upon acquisition of antigen, they mature and start migrating to the lymph node to present the antigen to naïve T cells. Depending on the context wherein the antigen is acquired, DCs will mature in a homeostatic or immunogenic manner. So far, the field is lacking proper tools to distinguish between the two maturation states. Most maturation markers are shared between the two states and therefore inappropriate to use. Still, defining the proper maturation type is crucial as it determines how the DCs will instruct the T cells towards antigen expressing cells. In this study, we used a lipid nanoparticle (LNP)-based approach to steer DC maturation pathways in vivo. CITE-seq analysis allowed us to design a panel of flow cytometry markers that reliably annotates the two DC maturation states, as validated in an infection and in a tumor model. Furthermore, the data corroborated that uptake of empty LNPs in DCs induces their homeostatic maturation, in contrast to uptake of mRNA-LNPs or TLR ligand-adjuvanted LNPs, leading to distinct effector T cell outputs. This reveals that LNPs themselves are not being decoded as “danger” by cDC1s, and that the cargo is essential to provide adjuvants activity, which is highly relevant for targeted design of LNP-based therapies.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JW8DXWX9AZ5PG3EE4WK3MVJF
- MLA
- Rennen, Sofie, et al. “Lipid Nanoparticles as a Tool to Dissect Dendritic Cell Maturation Pathways.” SSRN, 2025, doi:10.2139/ssrn.5212772.
- APA
- Rennen, S., Bosteels, V., De Nolf, C., Webb, G., Van Lil, K., Maréchal, S., … Janssens, S. (2025). Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways. https://doi.org/10.2139/ssrn.5212772
- Chicago author-date
- Rennen, Sofie, Victor Bosteels, Clint De Nolf, Greta Webb, Karo Van Lil, Sandra Maréchal, Jessica Vetters, et al. 2025. “Lipid Nanoparticles as a Tool to Dissect Dendritic Cell Maturation Pathways.” SSRN. https://doi.org/10.2139/ssrn.5212772.
- Chicago author-date (all authors)
- Rennen, Sofie, Victor Bosteels, Clint De Nolf, Greta Webb, Karo Van Lil, Sandra Maréchal, Jessica Vetters, Evelien Van De Velde, Farzaneh Fayazpour, Ria Roelandt, Niels Vandamme, Kevin Verstaen, Stefaan De Smedt, Karine Breckpot, Bruno De Geest, Rein Verbeke, Ine Lentacker, and Sophie Janssens. 2025. “Lipid Nanoparticles as a Tool to Dissect Dendritic Cell Maturation Pathways.” SSRN. doi:10.2139/ssrn.5212772.
- Vancouver
- 1.Rennen S, Bosteels V, De Nolf C, Webb G, Van Lil K, Maréchal S, et al. Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways. SSRN. 2025.
- IEEE
- [1]S. Rennen et al., “Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways,” SSRN. 2025.
@misc{01JW8DXWX9AZ5PG3EE4WK3MVJF,
abstract = {{Dendritic cells (DCs) are short-lived immune cells that continuously roam our body in search for foreign or self-antigens. Upon acquisition of antigen, they mature and start migrating to the lymph node to present the antigen to naïve T cells. Depending on the context wherein the antigen is acquired, DCs will mature in a homeostatic or immunogenic manner. So far, the field is lacking proper tools to distinguish between the two maturation states. Most maturation markers are shared between the two states and therefore inappropriate to use. Still, defining the proper maturation type is crucial as it determines how the DCs will instruct the T cells towards antigen expressing cells. In this study, we used a lipid nanoparticle (LNP)-based approach to steer DC maturation pathways in vivo. CITE-seq analysis allowed us to design a panel of flow cytometry markers that reliably annotates the two DC maturation states, as validated in an infection and in a tumor model. Furthermore, the data corroborated that uptake of empty LNPs in DCs induces their homeostatic maturation, in contrast to uptake of mRNA-LNPs or TLR ligand-adjuvanted LNPs, leading to distinct effector T cell outputs. This reveals that LNPs themselves are not being decoded as “danger” by cDC1s, and that the cargo is essential to provide adjuvants activity, which is highly relevant for targeted design of LNP-based therapies.}},
author = {{Rennen, Sofie and Bosteels, Victor and De Nolf, Clint and Webb, Greta and Van Lil, Karo and Maréchal, Sandra and Vetters, Jessica and Van De Velde, Evelien and Fayazpour, Farzaneh and Roelandt, Ria and Vandamme, Niels and Verstaen, Kevin and De Smedt, Stefaan and Breckpot, Karine and De Geest, Bruno and Verbeke, Rein and Lentacker, Ine and Janssens, Sophie}},
language = {{eng}},
series = {{SSRN}},
title = {{Lipid nanoparticles as a tool to dissect dendritic cell maturation pathways}},
url = {{http://doi.org/10.2139/ssrn.5212772}},
year = {{2025}},
}
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