SlBBX26 regulates vegetative growth, flowering and fruit development through the modulation of SlPIF4
- Author
- Juliene dos Reis Moreira, Lumi Shiose, Bruno Silvestre Lira, Gabriel Ponciano, Raquel Tsu Ay Wu, Mateus Henrique Vicente, Maria José Oliveira, Diego Demarco, Fabio Tebaldi Silveira Nogueira, Alain Goossens (UGent) , Luciano Freschi and Magdalena Rossi
- Organization
- Project
-
- Design of novel chemical-molecular tools to enlarge and adapt the endoplasmic reticulum of crop and medicinal plants for superior production of bioactive metabolites
- Regulatory proteins acting at the crossroads of light and jasmonate signaling: characterization of their molecular function and their impact on crop quality
- Abstract
- The BBX family of transcription factors regulate several physiological processes during plant vegetative and reproductive development, bridging light signaling to hormones metabolism. Here, we do an in-depth functional characterization of the tomato SlBBX26 (Solyc10g006750) gene by generating and phenotyping CRISPR/Cas9-mediated genome-edited and RNAi-mediated knockdown lines. We demonstrate that SlBBX26 regulates the negative factor of light signaling, PHYTOCHROME INTERACTING PROTEIN 4 (PIF4), which in turn modulates vegetative growth and flowering by controlling gibberellins (GAs) biosynthetic genes expression and signaling, respectively. Moreover, SlBBX26 regulates fruit growth modulating the expression of cell division- and expansion-related genes. SlBBX26 also influences fruit chloroplast ultrastructure and metabolism in an SlPIF4-mediated manner, leading to alterations in thylakoid stacking, plastoglobuli size, and chlorophyll content, through the regulation of genes involved in chloroplast differentiation and chlorophyll degradation. Finally, SlBBX26-SlPIF4 heterodimer is required to control GA and auxin signaling cascades, triggering the onset of fruit ripening. As such, our findings unveil how BBX proteins contribute to the regulation of main agronomic traits.
- Keywords
- tomato, Solanum lycopersicum, BBX proteins, flowering, ripening, gibberellins, INDUCED LEAF SENESCENCE, TOCOPHEROL BIOSYNTHESIS, GIBBERELLIC-ACID, GENE-EXPRESSION, DOWN-REGULATION, TRANSCRIPTION, LIGHT, PROTEIN, DECELERATION, INDUCTION
Downloads
-
1-s2.0-S0981942825005947-main.pdf
- full text (Accepted manuscript)
- |
- open access
- |
- |
- 2.09 MB
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 8.90 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JW6S5R5BFBTA2JQSB3F7P77Q
- MLA
- dos Reis Moreira, Juliene, et al. “SlBBX26 Regulates Vegetative Growth, Flowering and Fruit Development through the Modulation of SlPIF4.” PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 226, 2025, doi:10.1016/j.plaphy.2025.110066.
- APA
- dos Reis Moreira, J., Shiose, L., Lira, B. S., Ponciano, G., Wu, R. T. A., Vicente, M. H., … Rossi, M. (2025). SlBBX26 regulates vegetative growth, flowering and fruit development through the modulation of SlPIF4. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 226. https://doi.org/10.1016/j.plaphy.2025.110066
- Chicago author-date
- Reis Moreira, Juliene dos, Lumi Shiose, Bruno Silvestre Lira, Gabriel Ponciano, Raquel Tsu Ay Wu, Mateus Henrique Vicente, Maria José Oliveira, et al. 2025. “SlBBX26 Regulates Vegetative Growth, Flowering and Fruit Development through the Modulation of SlPIF4.” PLANT PHYSIOLOGY AND BIOCHEMISTRY 226. https://doi.org/10.1016/j.plaphy.2025.110066.
- Chicago author-date (all authors)
- dos Reis Moreira, Juliene, Lumi Shiose, Bruno Silvestre Lira, Gabriel Ponciano, Raquel Tsu Ay Wu, Mateus Henrique Vicente, Maria José Oliveira, Diego Demarco, Fabio Tebaldi Silveira Nogueira, Alain Goossens, Luciano Freschi, and Magdalena Rossi. 2025. “SlBBX26 Regulates Vegetative Growth, Flowering and Fruit Development through the Modulation of SlPIF4.” PLANT PHYSIOLOGY AND BIOCHEMISTRY 226. doi:10.1016/j.plaphy.2025.110066.
- Vancouver
- 1.dos Reis Moreira J, Shiose L, Lira BS, Ponciano G, Wu RTA, Vicente MH, et al. SlBBX26 regulates vegetative growth, flowering and fruit development through the modulation of SlPIF4. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 2025;226.
- IEEE
- [1]J. dos Reis Moreira et al., “SlBBX26 regulates vegetative growth, flowering and fruit development through the modulation of SlPIF4,” PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 226, 2025.
@article{01JW6S5R5BFBTA2JQSB3F7P77Q,
abstract = {{The BBX family of transcription factors regulate several physiological processes during plant vegetative and reproductive development, bridging light signaling to hormones metabolism. Here, we do an in-depth functional characterization of the tomato SlBBX26 (Solyc10g006750) gene by generating and phenotyping CRISPR/Cas9-mediated genome-edited and RNAi-mediated knockdown lines. We demonstrate that SlBBX26 regulates the negative factor of light signaling, PHYTOCHROME INTERACTING PROTEIN 4 (PIF4), which in turn modulates vegetative growth and flowering by controlling gibberellins (GAs) biosynthetic genes expression and signaling, respectively. Moreover, SlBBX26 regulates fruit growth modulating the expression of cell division- and expansion-related genes. SlBBX26 also influences fruit chloroplast ultrastructure and metabolism in an SlPIF4-mediated manner, leading to alterations in thylakoid stacking, plastoglobuli size, and chlorophyll content, through the regulation of genes involved in chloroplast differentiation and chlorophyll degradation. Finally, SlBBX26-SlPIF4 heterodimer is required to control GA and auxin signaling cascades, triggering the onset of fruit ripening. As such, our findings unveil how BBX proteins contribute to the regulation of main agronomic traits.}},
articleno = {{110066}},
author = {{dos Reis Moreira, Juliene and Shiose, Lumi and Lira, Bruno Silvestre and Ponciano, Gabriel and Wu, Raquel Tsu Ay and Vicente, Mateus Henrique and Oliveira, Maria José and Demarco, Diego and Nogueira, Fabio Tebaldi Silveira and Goossens, Alain and Freschi, Luciano and Rossi, Magdalena}},
issn = {{0981-9428}},
journal = {{PLANT PHYSIOLOGY AND BIOCHEMISTRY}},
keywords = {{tomato,Solanum lycopersicum,BBX proteins,flowering,ripening,gibberellins,INDUCED LEAF SENESCENCE,TOCOPHEROL BIOSYNTHESIS,GIBBERELLIC-ACID,GENE-EXPRESSION,DOWN-REGULATION,TRANSCRIPTION,LIGHT,PROTEIN,DECELERATION,INDUCTION}},
language = {{eng}},
pages = {{14}},
title = {{SlBBX26 regulates vegetative growth, flowering and fruit development through the modulation of SlPIF4}},
url = {{http://doi.org/10.1016/j.plaphy.2025.110066}},
volume = {{226}},
year = {{2025}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: