@article{01JVVNCNQE3BWRE3YMB3BYCQPZ,
  abstract     = {{Flexibility of cell walls is crucial to accommodate cell elongation and growth,
typically associated with the reorganization of cell wall polysaccharides. Seed
germination is a fast-paced developmental process in which cell wall adaptability
is highly required. The plant cell utilizes multiple strategies to obtain a flexible cell
wall and in part relies on cell wall-active enzymes to loosen both covalent and
non-covalent interactions between cell wall polysaccharides. OsAPSE is an
example of a cell wall-active enzyme originating from Japanese rice (Oryza
sativa subsp. Japonica) belonging to the glycoside hydrolase family 27 (GH27),
potentially active on the pectin–arabinogalactan protein O-glycan junction. We
provide insights into the biochemical and enzymatic properties of this protein,
characterized by the presence of a GH27 domain linked to a ricin-B-like domain.
Using small-scale production experiments in a cell-free protein synthesis system,
we demonstrated the catalytic activity of the recombinant OsAPSE towards
synthetic and natural substrates. Furthermore, subcellular localization analysis
and in silico data suggest that OsAPSE may undergo unconventional secretion to
the cell surface. We hypothesize that OsAPSE plays a role during rice seed
germination by removing terminal a-D-Galp and b-L-Arap moieties along the
pectin–arabinogalactan protein O-glycan network. This activity may abolish
non-covalent interactions between pectic rhamnogalacturonan I and Oglycans
of arabinogalactan proteins, contributing to cell wall relaxation for
growth during germination.}},
  articleno    = {{1588802}},
  author       = {{De Coninck, dr. ing. Tibo and Verbeke, Isabel and Rougé, Pierre and Desmet, Tom and Van Damme, Els}},
  issn         = {{1664-462X}},
  journal      = {{FRONTIERS IN PLANT SCIENCE}},
  keywords     = {{cell wall,glycoside hydrolase,a-D-galactopyranosidase,b-l-arabinopyranosidase,Oryza sativa,rice,arabinogalactan protein,germination,alpha-D-galactopyranosidase,beta-l-arabinopyranosidase,ALKALINE ALPHA-GALACTOSIDASE,BETA-L-ARABINOPYRANOSIDASE,GREEN FLUORESCENT PROTEIN,SDS-PAGE MIGRATION,NUCLEAR-LOCALIZATION,MOLECULAR-DYNAMICS,PHANEROCHAETE-CHRYSOSPORIUM,NICOTIANA-BENTHAMIANA,ENDOPLASMIC-RETICULUM,PHYLOGENETIC ANALYSIS}},
  language     = {{eng}},
  pages        = {{28}},
  title        = {{OsAPSE modulates non-covalent interactions between arabinogalactan protein O-glycans and pectin in rice cell walls}},
  url          = {{http://doi.org/10.3389/fpls.2025.1588802}},
  volume       = {{16}},
  year         = {{2025}},
}

Altmetric

View in Altmetric

Web of Science

Times cited: