@article{01JVC4GEJT1Z619Q2Y8NR2G4EH,
  abstract     = {{<jats:title>Abstract</jats:title><jats:p>Thiamin is a vital nutrient that acts as a cofactor for several enzymes primarily localized in the mitochondria. These thiamin-dependent enzymes are involved in energy metabolism, nucleic acid biosynthesis, and antioxidant machinery. The enzyme HMP-P kinase/thiamin monophosphate synthase (TH1) holds a key position in thiamin biosynthesis, being responsible for the phosphorylation of HMP-P into HMP-PP and for the condensation of HMP-PP and HET-P to form TMP. Through mathematical kinetic model, we have identified TH1 as a critical player for thiamin biofortification in rice. We further focused on the functional characterization of <jats:italic>OsTH1</jats:italic>. Sequence and gene expression analysis, along with phylogenetic studies, provided insights into <jats:italic>OsTH1</jats:italic> bifunctional features and evolution. The indispensable role of OsTH1 in thiamin biosynthesis was validated by heterologous expression of <jats:italic>OsTH1</jats:italic> and successful complementation of yeast knock-out mutants impaired in thiamin production. We also proved that the sole <jats:italic>OsTH1</jats:italic> overexpression in rice callus significantly improves B1 concentration, resulting in 50% increase in thiamin accumulation. Our study underscores the critical role of <jats:italic>OsTH1</jats:italic> in thiamin biosynthesis, shedding light on its bifunctional nature and evolutionary significance. The significant enhancement of thiamin accumulation in rice callus upon <jats:italic>OsTH1</jats:italic> overexpression constitutes evidence of its potential application in biofortification strategies.</jats:p>}},
  articleno    = {{13591}},
  author       = {{Vicente Faustino, Maria and Lourenço, Tiago and Strobbe, Simon and Cao, Da and Fonseca, André and Rocha, Isabel and Van Der Straeten, Dominique and Oliveira, M. Margarida}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{12}},
  title        = {{OsTH1 is a key player in thiamin biosynthesis in rice}},
  url          = {{http://doi.org/10.1038/s41598-024-62326-2}},
  volume       = {{14}},
  year         = {{2024}},
}

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