Agrobacterium-mediated genetic transformation of Melia volkensii Gürke
- Author
- Nandini Bhogar Suresh (UGent) , Vladimir Kaigorodov (UGent) , titus Magomere and Stefaan Werbrouck (UGent)
- Organization
- Abstract
- Melia volkensii, a native dryland tree species in Eastern Africa, is extensively grown for its timber that resists termites, as well as for firewood and animal fodder. This study presents the first successful genetic transformation of Melia volkensii mediated by Agrobacterium tumefaciens. A new binary vector enabled the introduction of the reporter gene M24::eGFP resulting in robust gene expression. This breakthrough facilitates future genetic improvement of M. volkensii, including applications of CRISPR-Cas-9 technology, to develop superior varieties for reforestation and sustainable land management in arid regions.
- Keywords
- Agrobacterium tumefaciens, Binary vector, Genetic transformation, GFP, Melia volkensii, SHOOT REGENERATION, EXPRESSION, PLANTS, L., FLUORESCENCE, THIDIAZURON, HYGROMYCIN, VECTOR
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JV2FNSZNMCAF1EN7R0GQFGJ7
- MLA
- Bhogar Suresh, Nandini, et al. “Agrobacterium-Mediated Genetic Transformation of Melia Volkensii Gürke.” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 161, no. 2, 2025, doi:10.1007/s11240-025-03041-2.
- APA
- Bhogar Suresh, N., Kaigorodov, V., Magomere, titus, & Werbrouck, S. (2025). Agrobacterium-mediated genetic transformation of Melia volkensii Gürke. PLANT CELL TISSUE AND ORGAN CULTURE, 161(2). https://doi.org/10.1007/s11240-025-03041-2
- Chicago author-date
- Bhogar Suresh, Nandini, Vladimir Kaigorodov, titus Magomere, and Stefaan Werbrouck. 2025. “Agrobacterium-Mediated Genetic Transformation of Melia Volkensii Gürke.” PLANT CELL TISSUE AND ORGAN CULTURE 161 (2). https://doi.org/10.1007/s11240-025-03041-2.
- Chicago author-date (all authors)
- Bhogar Suresh, Nandini, Vladimir Kaigorodov, titus Magomere, and Stefaan Werbrouck. 2025. “Agrobacterium-Mediated Genetic Transformation of Melia Volkensii Gürke.” PLANT CELL TISSUE AND ORGAN CULTURE 161 (2). doi:10.1007/s11240-025-03041-2.
- Vancouver
- 1.Bhogar Suresh N, Kaigorodov V, Magomere titus, Werbrouck S. Agrobacterium-mediated genetic transformation of Melia volkensii Gürke. PLANT CELL TISSUE AND ORGAN CULTURE. 2025;161(2).
- IEEE
- [1]N. Bhogar Suresh, V. Kaigorodov, titus Magomere, and S. Werbrouck, “Agrobacterium-mediated genetic transformation of Melia volkensii Gürke,” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 161, no. 2, 2025.
@article{01JV2FNSZNMCAF1EN7R0GQFGJ7,
abstract = {{Melia volkensii, a native dryland tree species in Eastern Africa, is extensively grown for its timber that resists termites, as well as for firewood and animal fodder. This study presents the first successful genetic transformation of Melia volkensii mediated by Agrobacterium tumefaciens. A new binary vector enabled the introduction of the reporter gene M24::eGFP resulting in robust gene expression. This breakthrough facilitates future genetic improvement of M. volkensii, including applications of CRISPR-Cas-9 technology, to develop superior varieties for reforestation and sustainable land management in arid regions.}},
articleno = {{47}},
author = {{Bhogar Suresh, Nandini and Kaigorodov, Vladimir and Magomere, titus and Werbrouck, Stefaan}},
issn = {{0167-6857}},
journal = {{PLANT CELL TISSUE AND ORGAN CULTURE}},
keywords = {{Agrobacterium tumefaciens,Binary vector,Genetic transformation,GFP,Melia volkensii,SHOOT REGENERATION,EXPRESSION,PLANTS,L.,FLUORESCENCE,THIDIAZURON,HYGROMYCIN,VECTOR}},
language = {{eng}},
number = {{2}},
pages = {{8}},
title = {{Agrobacterium-mediated genetic transformation of Melia volkensii Gürke}},
url = {{http://doi.org/10.1007/s11240-025-03041-2}},
volume = {{161}},
year = {{2025}},
}
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