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Deoxynivalenol prediction and spatial mapping in wheat based on online hyperspectral imagery scanning

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Abstract
Deoxynivalenol (DON), a harmful mycotoxin produced by several Fusarium species, poses critical challenges to wheat production and food safety. However, reducing risks of human toxicity requires pre-harvest detection of DON concentration across different zones of a field. This study investigates the potential of integrating hyperspectral imaging (HSI) in the 400–1000 nm range with machine learning (ML) models for online field detection and mapping of DON contamination in wheat (Triticum aestivum). Using a tractor-mounted push-broom hyperspectral camera, spectral data were collected across four commercial fields in Lithuania and Belgium. A total of 76 wheat samples collected during crop scanning were analyzed for DON levels using liquid chromatography-mass spectrometry (LC-MS). Initial analysis of spectral data alone revealed relatively low classification accuracy, with light gradient boosting machine (LGBM) achieving 55.92 % and decision tree classifier (DTC) achieving 51.97 %. However, the inclusion of fusarium head blight (FHB) severity as an additional feature significantly improved performance, boosting accuracy to 90.79 % for LGBM (a 62.4 % increase) and 86.18 % for DTC (a 65.8 % increase). Moreover, the use of mutual information (MI) for feature selection enhanced model accuracy, achieving 93.42 % for LGBM and 90.13 % for DTC. Spatial mapping of DON contamination demonstrated fair to substantial agreement with ground truth maps, providing valuable tools for farmers to understand DON distribution and implement targeted harvesting strategy. This study highlights the potential of integrating online HSI, ML, and feature selection techniques, for pre-harvest DON detection and mapping, providing valuable information for reducing risks of human toxicity and improving the economic value of wheat grain.
Keywords
Deoxynivalenol, Mycotoxins, Hyperspectral imaging, Spatial distribution, Fusarium head blight, FUSARIUM HEAD BLIGHT, MUTUAL INFORMATION, WINTER-WHEAT, ACCUMULATION, MYCOTOXINS, AGREEMENT, HARVEST, MODELS, GRAIN

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MLA
Almoujahed, Mhd Baraa, et al. “Deoxynivalenol Prediction and Spatial Mapping in Wheat Based on Online Hyperspectral Imagery Scanning.” SMART AGRICULTURAL TECHNOLOGY, vol. 11, 2025, doi:10.1016/j.atech.2025.100947.
APA
Almoujahed, M. B., Apolo Apolo, E., Alhussein, M., Kazlauskas, M., Kriaučiūnienė, Z., Šarauskis, E., & Mouazen, A. (2025). Deoxynivalenol prediction and spatial mapping in wheat based on online hyperspectral imagery scanning. SMART AGRICULTURAL TECHNOLOGY, 11. https://doi.org/10.1016/j.atech.2025.100947
Chicago author-date
Almoujahed, Mhd Baraa, Enrique Apolo Apolo, Mohammad Alhussein, Marius Kazlauskas, Zita Kriaučiūnienė, Egidijus Šarauskis, and Abdul Mouazen. 2025. “Deoxynivalenol Prediction and Spatial Mapping in Wheat Based on Online Hyperspectral Imagery Scanning.” SMART AGRICULTURAL TECHNOLOGY 11. https://doi.org/10.1016/j.atech.2025.100947.
Chicago author-date (all authors)
Almoujahed, Mhd Baraa, Enrique Apolo Apolo, Mohammad Alhussein, Marius Kazlauskas, Zita Kriaučiūnienė, Egidijus Šarauskis, and Abdul Mouazen. 2025. “Deoxynivalenol Prediction and Spatial Mapping in Wheat Based on Online Hyperspectral Imagery Scanning.” SMART AGRICULTURAL TECHNOLOGY 11. doi:10.1016/j.atech.2025.100947.
Vancouver
1.
Almoujahed MB, Apolo Apolo E, Alhussein M, Kazlauskas M, Kriaučiūnienė Z, Šarauskis E, et al. Deoxynivalenol prediction and spatial mapping in wheat based on online hyperspectral imagery scanning. SMART AGRICULTURAL TECHNOLOGY. 2025;11.
IEEE
[1]
M. B. Almoujahed et al., “Deoxynivalenol prediction and spatial mapping in wheat based on online hyperspectral imagery scanning,” SMART AGRICULTURAL TECHNOLOGY, vol. 11, 2025.
@article{01JRWBBQHF5F994VVFXG1CJS8S,
  abstract     = {{Deoxynivalenol (DON), a harmful mycotoxin produced by several Fusarium species, poses critical challenges to wheat production and food safety. However, reducing risks of human toxicity requires pre-harvest detection of DON concentration across different zones of a field. This study investigates the potential of integrating hyperspectral imaging (HSI) in the 400–1000 nm range with machine learning (ML) models for online field detection and mapping of DON contamination in wheat (Triticum aestivum). Using a tractor-mounted push-broom hyperspectral camera, spectral data were collected across four commercial fields in Lithuania and Belgium. A total of 76 wheat samples collected during crop scanning were analyzed for DON levels using liquid chromatography-mass spectrometry (LC-MS). Initial analysis of spectral data alone revealed relatively low classification accuracy, with light gradient boosting machine (LGBM) achieving 55.92 % and decision tree classifier (DTC) achieving 51.97 %. However, the inclusion of fusarium head blight (FHB) severity as an additional feature significantly improved performance, boosting accuracy to 90.79 % for LGBM (a 62.4 % increase) and 86.18 % for DTC (a 65.8 % increase). Moreover, the use of mutual information (MI) for feature selection enhanced model accuracy, achieving 93.42 % for LGBM and 90.13 % for DTC. Spatial mapping of DON contamination demonstrated fair to substantial agreement with ground truth maps, providing valuable tools for farmers to understand DON distribution and implement targeted harvesting strategy. This study highlights the potential of integrating online HSI, ML, and feature selection techniques, for pre-harvest DON detection and mapping, providing valuable information for reducing risks of human toxicity and improving the economic value of wheat grain.}},
  articleno    = {{100947}},
  author       = {{Almoujahed, Mhd Baraa and Apolo Apolo, Enrique and Alhussein, Mohammad and Kazlauskas, Marius and Kriaučiūnienė, Zita and Šarauskis, Egidijus and Mouazen, Abdul}},
  issn         = {{2772-3755}},
  journal      = {{SMART AGRICULTURAL TECHNOLOGY}},
  keywords     = {{Deoxynivalenol,Mycotoxins,Hyperspectral imaging,Spatial distribution,Fusarium head blight,FUSARIUM HEAD BLIGHT,MUTUAL INFORMATION,WINTER-WHEAT,ACCUMULATION,MYCOTOXINS,AGREEMENT,HARVEST,MODELS,GRAIN}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{Deoxynivalenol prediction and spatial mapping in wheat based on online hyperspectral imagery scanning}},
  url          = {{http://doi.org/10.1016/j.atech.2025.100947}},
  volume       = {{11}},
  year         = {{2025}},
}

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