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DNA damage-associated vesicle production in Stenotrophomonas maltophilia is mediated by a cryptic tailocin endolysin

Darshan Chandramowli (UGent) , Jolien Vitse, Stephan Stremersch (UGent) , Koen Raemdonck (UGent) , Kevin Braeckmans (UGent) and Bart Devreese (UGent)
(2025) BIORXIV.
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Abstract
Like other Gram-negative bacteria, S. maltophilia is capable of producing membrane vesicles under normal growth conditions. The addition of certain exogenous triggers stimulates the production of vesicles, including those that are distinct from the archetypal outer membrane vesicle. In this study, we examine the effects of DNA damage on vesiculation, with a focus on the role of a bacteriophage-encoded endolysin. We demonstrate that deletion of the gene that encodes this protein (mal) negatively affects the vesiculation capacity of S. maltophilia. Further, we provide evidence that the spontaneous re-arrangement of shattered membrane fragments attributable to mal-induced explosive cell lysis results in the formation of predominantly explosive outer membrane vesicles, while only a minor portion are outer-inner membrane vesicles. Our findings expand on the current knowledge of (cryptic) tailocins in the biogenesis of vesicles in conditions of stress.

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Citation

Please use this url to cite or link to this publication:

MLA
Chandramowli, Darshan, et al. “DNA Damage-Associated Vesicle Production in Stenotrophomonas Maltophilia Is Mediated by a Cryptic Tailocin Endolysin.” BIORXIV, 2025, doi:10.1101/2025.03.20.644284.
APA
Chandramowli, D., Vitse, J., Stremersch, S., Raemdonck, K., Braeckmans, K., & Devreese, B. (2025). DNA damage-associated vesicle production in Stenotrophomonas maltophilia is mediated by a cryptic tailocin endolysin. https://doi.org/10.1101/2025.03.20.644284
Chicago author-date
Chandramowli, Darshan, Jolien Vitse, Stephan Stremersch, Koen Raemdonck, Kevin Braeckmans, and Bart Devreese. 2025. “DNA Damage-Associated Vesicle Production in Stenotrophomonas Maltophilia Is Mediated by a Cryptic Tailocin Endolysin.” BIORXIV. https://doi.org/10.1101/2025.03.20.644284.
Chicago author-date (all authors)
Chandramowli, Darshan, Jolien Vitse, Stephan Stremersch, Koen Raemdonck, Kevin Braeckmans, and Bart Devreese. 2025. “DNA Damage-Associated Vesicle Production in Stenotrophomonas Maltophilia Is Mediated by a Cryptic Tailocin Endolysin.” BIORXIV. doi:10.1101/2025.03.20.644284.
Vancouver
1.
Chandramowli D, Vitse J, Stremersch S, Raemdonck K, Braeckmans K, Devreese B. DNA damage-associated vesicle production in Stenotrophomonas maltophilia is mediated by a cryptic tailocin endolysin. BIORXIV. 2025.
IEEE
[1]
D. Chandramowli, J. Vitse, S. Stremersch, K. Raemdonck, K. Braeckmans, and B. Devreese, “DNA damage-associated vesicle production in Stenotrophomonas maltophilia is mediated by a cryptic tailocin endolysin,” BIORXIV. 2025.
@misc{01JQNM1YZHBJZ8J5JM7KC8GMEA,
  abstract     = {{Like other Gram-negative bacteria, S. maltophilia is capable of producing membrane vesicles under normal growth conditions. The addition of certain exogenous triggers stimulates the production of vesicles, including those that are distinct from the archetypal outer membrane vesicle. In this study, we examine the effects of DNA damage on vesiculation, with a focus on the role of a bacteriophage-encoded endolysin. We demonstrate that deletion of the gene that encodes this protein (mal) negatively affects the vesiculation capacity of S. maltophilia. Further, we provide evidence that the spontaneous re-arrangement of shattered membrane fragments attributable to mal-induced explosive cell lysis results in the formation of predominantly explosive outer membrane vesicles, while only a minor portion are outer-inner membrane vesicles. Our findings expand on the current knowledge of (cryptic) tailocins in the biogenesis of vesicles in conditions of stress.}},
  author       = {{Chandramowli, Darshan and Vitse, Jolien and Stremersch, Stephan and Raemdonck, Koen and Braeckmans, Kevin and Devreese, Bart}},
  language     = {{eng}},
  pages        = {{22}},
  series       = {{BIORXIV}},
  title        = {{DNA damage-associated vesicle production in Stenotrophomonas maltophilia is mediated by a cryptic tailocin endolysin}},
  url          = {{http://doi.org/10.1101/2025.03.20.644284}},
  year         = {{2025}},
}
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