Sputtered iridium oxide electrodes optimization and implementation for impedimetric identification of S. aureus and P. aeruginosa biofilms
- Author
- Francesco Grassi (UGent) , Charlotte Cums, Dieter Cuypers (UGent) , Rik Verplancke (UGent) , Thijs Vackier, David Schaubroeck (UGent) , Maxime Van Haeverbeke (UGent) , Hans Steenackers and Maaike Op de Beeck (UGent)
- Organization
- Project
- Abstract
- Electrochemical impedance spectroscopy (EIS) has been established as an effective technique for bacterial biofilm detection. Through the need for miniaturization, the application of novel electrode materials gains interest. In this study, we introduce Sputtered IRidium Oxide Film (SIROF) electrodes of varying sizes and geometries as sensors for biofilm detection. Pre-emptive cyclic voltammetry (pre-cycling) was used to transform as-sputtered anhydrous iridium oxide films into hydroxides, reducing the impedance and allowing the material to be adopted for miniaturized biofilm sensors. Our investigation showed that especially lower scan rates during this pre-cycling process reduced the interfacial impedance, hence optimizing electrode performance for this application. Using EIS in combination with pre-cycled SIROF electrodes, we detected biofilm growth within 24 hours and successfully distinguished between biofilms of S. aureus and P. aeruginosa. Additionally, we analyzed the influence of electrode size on biofilm detection and characterization. This study highlights SIROF electrodes as a promising platform for sensitive and scalable biofilm monitoring.
- Keywords
- SIROF, activation, pre-cycling, cyclic voltammetry, electrochemical impedance spectroscopy, biofilms, iridium oxide, EIS, NEURAL STIMULATION, ESCHERICHIA-COLI, CHARGE INJECTION, RAPID DETECTION, THIN-FILM, IMPEDANCE, PH, ARRAY, INFECTIONS, SENSOR
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Grassi 2025 J. Electrochem. Soc. 172 035501.pdf
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JPW0V0SJE21X65THK66NNCMJ
- MLA
- Grassi, Francesco, et al. “Sputtered Iridium Oxide Electrodes Optimization and Implementation for Impedimetric Identification of S. Aureus and P. Aeruginosa Biofilms.” JOURNAL OF THE ELECTROCHEMICAL SOCIETY, vol. 172, no. 3, 2025, doi:10.1149/1945-7111/adbdf2.
- APA
- Grassi, F., Cums, C., Cuypers, D., Verplancke, R., Vackier, T., Schaubroeck, D., … Op de Beeck, M. (2025). Sputtered iridium oxide electrodes optimization and implementation for impedimetric identification of S. aureus and P. aeruginosa biofilms. JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 172(3). https://doi.org/10.1149/1945-7111/adbdf2
- Chicago author-date
- Grassi, Francesco, Charlotte Cums, Dieter Cuypers, Rik Verplancke, Thijs Vackier, David Schaubroeck, Maxime Van Haeverbeke, Hans Steenackers, and Maaike Op de Beeck. 2025. “Sputtered Iridium Oxide Electrodes Optimization and Implementation for Impedimetric Identification of S. Aureus and P. Aeruginosa Biofilms.” JOURNAL OF THE ELECTROCHEMICAL SOCIETY 172 (3). https://doi.org/10.1149/1945-7111/adbdf2.
- Chicago author-date (all authors)
- Grassi, Francesco, Charlotte Cums, Dieter Cuypers, Rik Verplancke, Thijs Vackier, David Schaubroeck, Maxime Van Haeverbeke, Hans Steenackers, and Maaike Op de Beeck. 2025. “Sputtered Iridium Oxide Electrodes Optimization and Implementation for Impedimetric Identification of S. Aureus and P. Aeruginosa Biofilms.” JOURNAL OF THE ELECTROCHEMICAL SOCIETY 172 (3). doi:10.1149/1945-7111/adbdf2.
- Vancouver
- 1.Grassi F, Cums C, Cuypers D, Verplancke R, Vackier T, Schaubroeck D, et al. Sputtered iridium oxide electrodes optimization and implementation for impedimetric identification of S. aureus and P. aeruginosa biofilms. JOURNAL OF THE ELECTROCHEMICAL SOCIETY. 2025;172(3).
- IEEE
- [1]F. Grassi et al., “Sputtered iridium oxide electrodes optimization and implementation for impedimetric identification of S. aureus and P. aeruginosa biofilms,” JOURNAL OF THE ELECTROCHEMICAL SOCIETY, vol. 172, no. 3, 2025.
@article{01JPW0V0SJE21X65THK66NNCMJ,
abstract = {{Electrochemical impedance spectroscopy (EIS) has been established as an effective technique for bacterial biofilm detection. Through the need for miniaturization, the application of novel electrode materials gains interest. In this study, we introduce Sputtered IRidium Oxide Film (SIROF) electrodes of varying sizes and geometries as sensors for biofilm detection. Pre-emptive cyclic voltammetry (pre-cycling) was used to transform as-sputtered anhydrous iridium oxide films into hydroxides, reducing the impedance and allowing the material to be adopted for miniaturized biofilm sensors. Our investigation showed that especially lower scan rates during this pre-cycling process reduced the interfacial impedance, hence optimizing electrode performance for this application. Using EIS in combination with pre-cycled SIROF electrodes, we detected biofilm growth within 24 hours and successfully distinguished between biofilms of S. aureus and P. aeruginosa. Additionally, we analyzed the influence of electrode size on biofilm detection and characterization. This study highlights SIROF electrodes as a promising platform for sensitive and scalable biofilm monitoring.}},
articleno = {{035501}},
author = {{Grassi, Francesco and Cums, Charlotte and Cuypers, Dieter and Verplancke, Rik and Vackier, Thijs and Schaubroeck, David and Van Haeverbeke, Maxime and Steenackers, Hans and Op de Beeck, Maaike}},
issn = {{0013-4651}},
journal = {{JOURNAL OF THE ELECTROCHEMICAL SOCIETY}},
keywords = {{SIROF,activation,pre-cycling,cyclic voltammetry,electrochemical impedance spectroscopy,biofilms,iridium oxide,EIS,NEURAL STIMULATION,ESCHERICHIA-COLI,CHARGE INJECTION,RAPID DETECTION,THIN-FILM,IMPEDANCE,PH,ARRAY,INFECTIONS,SENSOR}},
language = {{eng}},
number = {{3}},
pages = {{12}},
title = {{Sputtered iridium oxide electrodes optimization and implementation for impedimetric identification of S. aureus and P. aeruginosa biofilms}},
url = {{http://doi.org/10.1149/1945-7111/adbdf2}},
volume = {{172}},
year = {{2025}},
}
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