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PRC1 and PRC2 proximal interactome in mouse embryonic stem cells

Dick W. Zijlmans, Suzan Stelloo, Danique Bax, Yavor Yordanov, Pien Toebosch, Maximilian W.D. Raas, Sigrid Verhelst (UGent) , Lieke A. Lamers, Marijke P.A. Baltissen, Pascal W.T.C. Jansen, et al.
(2025) CELL REPORTS. 44(3).
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Abstract
Polycomb repressive complexes PRC1 and PRC2 control lineage-specific gene silencing during early embryogenesis. To better understand Polycomb biology, we profile the proximal interactome (proxeome) of multiple PRC1 and PRC2 subunits in mouse embryonic stem cells (mESCs). This analysis identifies >100 proteins proximal to PRC1 and PRC2, including transcription factors and RNA-binding proteins. Notably, approximately half of the PRC2 interactors overlap with PRC1. Pluripotency-associated factors, including NANOG, colocalize with PRC2 at specific genomic sites. Following PRC2 disruption, NANOG relocalizes to other genomic regions. Interestingly, we identify PRC1 members in PRC2 proxeomes but not reciprocally. This suggests that PRC1 and PRC2 may have independent functions in addition to their cooperative roles in establishing H3K27me3-marked chromatin domains. Finally, we compare PRC2 proxeomes across different cellular contexts, including ground-state mESCs, serum-cultured mESCs, and embryoid bodies. These analyses provide a comprehensive resource, enhancing our understanding of Polycomb biology and its dynamic role across developmental states.

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MLA
Zijlmans, Dick W., et al. “PRC1 and PRC2 Proximal Interactome in Mouse Embryonic Stem Cells.” CELL REPORTS, vol. 44, no. 3, 2025, doi:10.1016/j.celrep.2025.115362.
APA
Zijlmans, D. W., Stelloo, S., Bax, D., Yordanov, Y., Toebosch, P., Raas, M. W. D., … Vermeulen, M. (2025). PRC1 and PRC2 proximal interactome in mouse embryonic stem cells. CELL REPORTS, 44(3). https://doi.org/10.1016/j.celrep.2025.115362
Chicago author-date
Zijlmans, Dick W., Suzan Stelloo, Danique Bax, Yavor Yordanov, Pien Toebosch, Maximilian W.D. Raas, Sigrid Verhelst, et al. 2025. “PRC1 and PRC2 Proximal Interactome in Mouse Embryonic Stem Cells.” CELL REPORTS 44 (3). https://doi.org/10.1016/j.celrep.2025.115362.
Chicago author-date (all authors)
Zijlmans, Dick W., Suzan Stelloo, Danique Bax, Yavor Yordanov, Pien Toebosch, Maximilian W.D. Raas, Sigrid Verhelst, Lieke A. Lamers, Marijke P.A. Baltissen, Pascal W.T.C. Jansen, Guido van Mierlo, Maarten Dhaenens, Hendrik Marks, and Michiel Vermeulen. 2025. “PRC1 and PRC2 Proximal Interactome in Mouse Embryonic Stem Cells.” CELL REPORTS 44 (3). doi:10.1016/j.celrep.2025.115362.
Vancouver
1.
Zijlmans DW, Stelloo S, Bax D, Yordanov Y, Toebosch P, Raas MWD, et al. PRC1 and PRC2 proximal interactome in mouse embryonic stem cells. CELL REPORTS. 2025;44(3).
IEEE
[1]
D. W. Zijlmans et al., “PRC1 and PRC2 proximal interactome in mouse embryonic stem cells,” CELL REPORTS, vol. 44, no. 3, 2025.
@article{01JP82H3G47Y2X451RS74MP314,
  abstract     = {{Polycomb repressive complexes PRC1 and PRC2 control lineage-specific gene silencing during early embryogenesis. To better understand Polycomb biology, we profile the proximal interactome (proxeome) of multiple PRC1 and PRC2 subunits in mouse embryonic stem cells (mESCs). This analysis identifies >100 proteins proximal to PRC1 and PRC2, including transcription factors and RNA-binding proteins. Notably, approximately half of the PRC2 interactors overlap with PRC1. Pluripotency-associated factors, including NANOG, colocalize with PRC2 at specific genomic sites. Following PRC2 disruption, NANOG relocalizes to other genomic regions. Interestingly, we identify PRC1 members in PRC2 proxeomes but not reciprocally. This suggests that PRC1 and PRC2 may have independent functions in addition to their cooperative roles in establishing H3K27me3-marked chromatin domains. Finally, we compare PRC2 proxeomes across different cellular contexts, including ground-state mESCs, serum-cultured mESCs, and embryoid bodies. These analyses provide a comprehensive resource, enhancing our understanding of Polycomb biology and its dynamic role across developmental states.}},
  articleno    = {{115362}},
  author       = {{Zijlmans, Dick W. and Stelloo, Suzan and Bax, Danique and Yordanov, Yavor and Toebosch, Pien and Raas, Maximilian W.D. and Verhelst, Sigrid and Lamers, Lieke A. and Baltissen, Marijke P.A. and Jansen, Pascal W.T.C. and van Mierlo, Guido and Dhaenens, Maarten and Marks, Hendrik and Vermeulen, Michiel}},
  issn         = {{2211-1247}},
  journal      = {{CELL REPORTS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{23}},
  title        = {{PRC1 and PRC2 proximal interactome in mouse embryonic stem cells}},
  url          = {{http://doi.org/10.1016/j.celrep.2025.115362}},
  volume       = {{44}},
  year         = {{2025}},
}
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