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Embedded system for responsive optogenetic control of spontaneous seizures in a preclinical temporal lobe epilepsy model

Sofie Lasure (UGent) , Lien De Schaepmeester (UGent) , Sielke Caestecker (UGent) , Jeroen Spanoghe (UGent) , Marijke Vergaelen (UGent) , Rik Verplancke (UGent) , Johannes Vierock, Robrecht Raedt (UGent) and Pieter Bauwens (UGent)
(2025) Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1. p.88-99
Author
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Abstract
Aims: Responsive neuromodulation employing optogenetics is a promising therapy which provides spatial and temporal specificity for temporal lobe epilepsy (TLE), a neurological disorder characterised by the occurrence of spontaneous seizures. In this study, we evaluated whether seizures could be detected with a self-designed minimalist embedded system and terminated through activation of the WiChR opsin in the intrahippocampal kainic acid (IHKA) mouse model of temporal lobe epilepsy. Methods: Mice were injected in the hippocampus with kainic acid to simulate TLE and with the AAV2/9 viral vector to induce expression of the WiChR opsin. Intracranial EEG was recorded and processed with a low-power microcontroller to detect the seizure via the amplitude correlation metric. Upon detection, a 473nm Light Emitting Diode (LED) was activated to illuminate the hippocampus through an optrode. Results: It was possible to responsively illuminate seizures with the embedded system and achieve a significant reduction in seizure duration with a pulse train of 10 Hz, 5 ms, 10 mW for 90 s. A brief parameter study was performed although preliminary results were inconclusive. Conclusions: In this study, we prove that we can responsively suppress seizures in the IHKA mouse model within the limitations of a minimalist embedded system. Additionally, the WiChR opsin has been demonstrated to have a high potential for efficient seizure suppression with limited illumination.
Keywords
Epilepsy, Responsive neuromodulation, Optogenetics, Embedded system, MSP430 microcontroller

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MLA
Lasure, Sofie, et al. “Embedded System for Responsive Optogenetic Control of Spontaneous Seizures in a Preclinical Temporal Lobe Epilepsy Model.” Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1, SCITEPRESS - Science and Technology Publications, 2025, pp. 88–99, doi:10.5220/0013377000003911.
APA
Lasure, S., De Schaepmeester, L., Caestecker, S., Spanoghe, J., Vergaelen, M., Verplancke, R., … Bauwens, P. (2025). Embedded system for responsive optogenetic control of spontaneous seizures in a preclinical temporal lobe epilepsy model. Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1, 88–99. https://doi.org/10.5220/0013377000003911
Chicago author-date
Lasure, Sofie, Lien De Schaepmeester, Sielke Caestecker, Jeroen Spanoghe, Marijke Vergaelen, Rik Verplancke, Johannes Vierock, Robrecht Raedt, and Pieter Bauwens. 2025. “Embedded System for Responsive Optogenetic Control of Spontaneous Seizures in a Preclinical Temporal Lobe Epilepsy Model.” In Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1, 88–99. SCITEPRESS - Science and Technology Publications. https://doi.org/10.5220/0013377000003911.
Chicago author-date (all authors)
Lasure, Sofie, Lien De Schaepmeester, Sielke Caestecker, Jeroen Spanoghe, Marijke Vergaelen, Rik Verplancke, Johannes Vierock, Robrecht Raedt, and Pieter Bauwens. 2025. “Embedded System for Responsive Optogenetic Control of Spontaneous Seizures in a Preclinical Temporal Lobe Epilepsy Model.” In Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1, 88–99. SCITEPRESS - Science and Technology Publications. doi:10.5220/0013377000003911.
Vancouver
1.
Lasure S, De Schaepmeester L, Caestecker S, Spanoghe J, Vergaelen M, Verplancke R, et al. Embedded system for responsive optogenetic control of spontaneous seizures in a preclinical temporal lobe epilepsy model. In: Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1. SCITEPRESS - Science and Technology Publications; 2025. p. 88–99.
IEEE
[1]
S. Lasure et al., “Embedded system for responsive optogenetic control of spontaneous seizures in a preclinical temporal lobe epilepsy model,” in Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1, Porto, Portugal, 2025, pp. 88–99.
@inproceedings{01JP7A545CYQ6MWSDKQG24GJGB,
  abstract     = {{Aims: Responsive neuromodulation employing optogenetics is a promising therapy which provides spatial and temporal specificity for temporal lobe epilepsy (TLE), a neurological disorder characterised by the occurrence of spontaneous seizures. In this study, we evaluated whether seizures could be detected with a self-designed minimalist embedded system and terminated through activation of the WiChR opsin in the intrahippocampal kainic acid (IHKA) mouse model of temporal lobe epilepsy. Methods: Mice were injected in the hippocampus with kainic acid to simulate TLE and with the AAV2/9 viral vector to induce expression of the WiChR opsin. Intracranial EEG was recorded and processed with a low-power microcontroller to detect the seizure via the amplitude correlation metric. Upon detection, a 473nm Light Emitting Diode (LED) was activated to illuminate the hippocampus through an optrode. Results: It was possible to responsively illuminate seizures with the embedded system and achieve a significant reduction in seizure duration with a pulse train of 10 Hz, 5 ms, 10 mW for 90 s. A brief parameter study was performed although preliminary results were inconclusive. Conclusions: In this study, we prove that we can responsively suppress seizures in the IHKA mouse model within the limitations of a minimalist embedded system. Additionally, the WiChR opsin has been demonstrated to have a high potential for efficient seizure suppression with limited illumination.}},
  author       = {{Lasure, Sofie and De Schaepmeester, Lien and Caestecker, Sielke and Spanoghe, Jeroen and Vergaelen, Marijke and Verplancke, Rik and Vierock, Johannes and Raedt, Robrecht and Bauwens, Pieter}},
  booktitle    = {{Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1}},
  keywords     = {{Epilepsy,Responsive neuromodulation,Optogenetics,Embedded system,MSP430 microcontroller}},
  language     = {{eng}},
  location     = {{Porto, Portugal}},
  pages        = {{88--99}},
  publisher    = {{SCITEPRESS - Science and Technology Publications}},
  title        = {{Embedded system for responsive optogenetic control of spontaneous seizures in a preclinical temporal lobe epilepsy model}},
  url          = {{http://doi.org/10.5220/0013377000003911}},
  year         = {{2025}},
}
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