Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants
- Author
- Jessica Pérez-Sancho, Marija Smokvarska, Gwennogan Dubois, Marie Glavier, Sujith Sritharan, Tatiana S. Moraes, Hortense Moreau, Victor Dietrich, Matthieu P. Platre, Andrea Paterlini, Ziqiang P. Li, Laetitia Fouillen, Magali S. Grison, Pepe Cana-Quijada, Françoise Immel, Valerie Wattelet, Mathieu Ducros, Lysiane Brocard, Clément Chambaud, Yongming Luo (UGent) , Priya Ramakrishna, Vincent Bayle, Linnka Lefebvre-Legendre, Stéphane Claverol, Matej Zabrady, Pascal G.P. Martin, Wolfgang Busch, Marie Barberon, Jens Tilsner, Yrjö Helariutta, Eugenia Russinova (UGent) , Antoine Taly, Yvon Jaillais and Emmanuelle M. Bayer
- Organization
- Project
- Abstract
- Membrane contact sites (MCSs) are fundamental for intracellular communication, but their role in intercellular communication remains unexplored. We show that in plants, plasmodesmata communication bridges function as atypical endoplasmic reticulum (ER)-plasma membrane (PM) tubular MCSs, operating at cell-cell interfaces. Similar to other MCSs, ER-PM apposition is controlled by a protein-lipid tethering complex, but uniquely, this serves intercellular communication. Combining high-resolution microscopy, molecular dynamics, and pharmacological and genetic approaches, we show that cell-cell trafficking is modulated through the combined action of multiple C2 domains transmembrane domain proteins (MCTPs) 3, 4, and 6 ER-PM tethers and phosphatidylinositol-4-phosphate (PI4P) lipid. Graded PI4P amounts regulate MCTP docking to the PM, their plasmodesmata localization, and cell-cell permeability. SAC7, an ER-localized PI4P-phosphatase, regulates MCTP4 accumulation at plasmodesmata and modulates cell-cell trafficking capacity in a cell-type-specific manner. Our findings expand MCS functions in information transmission from intracellular to intercellular cellular activities.
- Keywords
- MCTP, endoplasmic reticulum plasma membrane, intercellular communication, membrane contact sites, phosphoinositide, plant biology, plasmodesmata, SYMPLASTIC TRAFFICKING, CALLOSE DEPOSITION, ER, FORM, PROMOTER, DYNAMICS, MERISTEM, PLATFORM, FLUX
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JMVPHQMXAVRNCX41NBBATKSV
- MLA
- Pérez-Sancho, Jessica, et al. “Plasmodesmata Act as Unconventional Membrane Contact Sites Regulating Intercellular Molecular Exchange in Plants.” CELL, vol. 188, no. 4, 2025, pp. 958-977.e23, doi:10.1016/j.cell.2024.11.034.
- APA
- Pérez-Sancho, J., Smokvarska, M., Dubois, G., Glavier, M., Sritharan, S., Moraes, T. S., … Bayer, E. M. (2025). Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants. CELL, 188(4), 958-977.e23. https://doi.org/10.1016/j.cell.2024.11.034
- Chicago author-date
- Pérez-Sancho, Jessica, Marija Smokvarska, Gwennogan Dubois, Marie Glavier, Sujith Sritharan, Tatiana S. Moraes, Hortense Moreau, et al. 2025. “Plasmodesmata Act as Unconventional Membrane Contact Sites Regulating Intercellular Molecular Exchange in Plants.” CELL 188 (4): 958-977.e23. https://doi.org/10.1016/j.cell.2024.11.034.
- Chicago author-date (all authors)
- Pérez-Sancho, Jessica, Marija Smokvarska, Gwennogan Dubois, Marie Glavier, Sujith Sritharan, Tatiana S. Moraes, Hortense Moreau, Victor Dietrich, Matthieu P. Platre, Andrea Paterlini, Ziqiang P. Li, Laetitia Fouillen, Magali S. Grison, Pepe Cana-Quijada, Françoise Immel, Valerie Wattelet, Mathieu Ducros, Lysiane Brocard, Clément Chambaud, Yongming Luo, Priya Ramakrishna, Vincent Bayle, Linnka Lefebvre-Legendre, Stéphane Claverol, Matej Zabrady, Pascal G.P. Martin, Wolfgang Busch, Marie Barberon, Jens Tilsner, Yrjö Helariutta, Eugenia Russinova, Antoine Taly, Yvon Jaillais, and Emmanuelle M. Bayer. 2025. “Plasmodesmata Act as Unconventional Membrane Contact Sites Regulating Intercellular Molecular Exchange in Plants.” CELL 188 (4): 958-977.e23. doi:10.1016/j.cell.2024.11.034.
- Vancouver
- 1.Pérez-Sancho J, Smokvarska M, Dubois G, Glavier M, Sritharan S, Moraes TS, et al. Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants. CELL. 2025;188(4):958-977.e23.
- IEEE
- [1]J. Pérez-Sancho et al., “Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants,” CELL, vol. 188, no. 4, pp. 958-977.e23, 2025.
@article{01JMVPHQMXAVRNCX41NBBATKSV,
abstract = {{Membrane contact sites (MCSs) are fundamental for intracellular communication, but their role in intercellular communication remains unexplored. We show that in plants, plasmodesmata communication bridges function as atypical endoplasmic reticulum (ER)-plasma membrane (PM) tubular MCSs, operating at cell-cell interfaces. Similar to other MCSs, ER-PM apposition is controlled by a protein-lipid tethering complex, but uniquely, this serves intercellular communication. Combining high-resolution microscopy, molecular dynamics, and pharmacological and genetic approaches, we show that cell-cell trafficking is modulated through the combined action of multiple C2 domains transmembrane domain proteins (MCTPs) 3, 4, and 6 ER-PM tethers and phosphatidylinositol-4-phosphate (PI4P) lipid. Graded PI4P amounts regulate MCTP docking to the PM, their plasmodesmata localization, and cell-cell permeability. SAC7, an ER-localized PI4P-phosphatase, regulates MCTP4 accumulation at plasmodesmata and modulates cell-cell trafficking capacity in a cell-type-specific manner. Our findings expand MCS functions in information transmission from intracellular to intercellular cellular activities.}},
author = {{Pérez-Sancho, Jessica and Smokvarska, Marija and Dubois, Gwennogan and Glavier, Marie and Sritharan, Sujith and Moraes, Tatiana S. and Moreau, Hortense and Dietrich, Victor and Platre, Matthieu P. and Paterlini, Andrea and Li, Ziqiang P. and Fouillen, Laetitia and Grison, Magali S. and Cana-Quijada, Pepe and Immel, Françoise and Wattelet, Valerie and Ducros, Mathieu and Brocard, Lysiane and Chambaud, Clément and Luo, Yongming and Ramakrishna, Priya and Bayle, Vincent and Lefebvre-Legendre, Linnka and Claverol, Stéphane and Zabrady, Matej and Martin, Pascal G.P. and Busch, Wolfgang and Barberon, Marie and Tilsner, Jens and Helariutta, Yrjö and Russinova, Eugenia and Taly, Antoine and Jaillais, Yvon and Bayer, Emmanuelle M.}},
issn = {{0092-8674}},
journal = {{CELL}},
keywords = {{MCTP,endoplasmic reticulum plasma membrane,intercellular communication,membrane contact sites,phosphoinositide,plant biology,plasmodesmata,SYMPLASTIC TRAFFICKING,CALLOSE DEPOSITION,ER,FORM,PROMOTER,DYNAMICS,MERISTEM,PLATFORM,FLUX}},
language = {{eng}},
number = {{4}},
pages = {{958--977.e23}},
title = {{Plasmodesmata act as unconventional membrane contact sites regulating intercellular molecular exchange in plants}},
url = {{http://doi.org/10.1016/j.cell.2024.11.034}},
volume = {{188}},
year = {{2025}},
}
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