OPENPichia : licence-free Komagataella phaffii chassis strains and toolkit for protein expression
- Author
- Katrien Claes (UGent) , Dries Van Herpe (UGent) , Robin Vanluchene, Charlotte Roels (UGent) , Berre Van Moer (UGent) , Elise Wyseure (UGent) , Kristof Vandewalle, Hannah Eeckhaut (UGent) , Semiramis Yilmaz (UGent) , Sandrine Vanmarcke (UGent) , Erhan Citak (UGent) , Daria Fijalkowska (UGent) , Hendrik Grootaert (UGent) , Chiara Lonigro, Leander Meuris (UGent) , Gitte Michielsen (UGent) , Justine Naessens (UGent) , Loes van Schie (UGent) , Riet De Rycke (UGent) , Michiel De Bruyne (UGent) , Peter Borghgraef (UGent) and Nico Callewaert (UGent)
- Organization
- Project
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- 3S018916
- Understanding, optimizing and expanding target space of yeast-secreted, edible antibodies.
- GlyConnect: developing glyco-active enzyme reagents for therapeutic glycoprotein functionality enhancement
- Development of a novel antibody-based platform for lysosome-mediated targeted protein degradation
- SECRiFY: a new tool for studying eukaryotic protein secretion.
- Improving monoclonal antibody production in glycosylationhumanized Pichia pastoris strains
- Abstract
- The industrial yeast Komagataella phaffii (formerly named Pichia pastoris) is commonly used to synthesize recombinant proteins, many of which are used as human therapeutics or in food. However, the basic strain, named NRRL Y-11430, from which all commercial hosts are derived, is not available without restrictions on its use. Comparative genome sequencing leaves little doubt that NRRL Y-11430 is derived from a K. phaffii type strain deposited in the UC Davis Phaff Yeast Strain Collection in 1954. We analysed four equivalent type strains in several culture collections and identified the NCYC 2543 strain, from which we started to develop an open-access Pichia chassis strain that anyone can use to produce recombinant proteins to industry standards. NRRL Y-11430 is readily transformable, which we found to be due to a HOC1 open-reading-frame truncation that alters cell-wall mannan. We introduced the HOC1 open-reading-frame truncation into NCYC 2543, which increased the transformability and improved secretion of some but not all of our tested proteins. We provide our genome-sequenced type strain, the hoc1(tr) derivative that we named OPENPichia as well as a synthetic, modular expression vector toolkit under liberal end-user distribution licences as an unencumbered OPENPichia resource for the microbial biotechnology community.
- Keywords
- PICHIA-PASTORIS, MICROBIAL-METABOLISM, YEAST, SEQUENCE, METHANOL
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JJ1KWX4ZK4A2994N8948N8QP
- MLA
- Claes, Katrien, et al. “OPENPichia : Licence-Free Komagataella Phaffii Chassis Strains and Toolkit for Protein Expression.” NATURE MICROBIOLOGY, vol. 9, no. 3, 2024, pp. 864–76, doi:10.1038/s41564-023-01574-w.
- APA
- Claes, K., Van Herpe, D., Vanluchene, R., Roels, C., Van Moer, B., Wyseure, E., … Callewaert, N. (2024). OPENPichia : licence-free Komagataella phaffii chassis strains and toolkit for protein expression. NATURE MICROBIOLOGY, 9(3), 864–876. https://doi.org/10.1038/s41564-023-01574-w
- Chicago author-date
- Claes, Katrien, Dries Van Herpe, Robin Vanluchene, Charlotte Roels, Berre Van Moer, Elise Wyseure, Kristof Vandewalle, et al. 2024. “OPENPichia : Licence-Free Komagataella Phaffii Chassis Strains and Toolkit for Protein Expression.” NATURE MICROBIOLOGY 9 (3): 864–76. https://doi.org/10.1038/s41564-023-01574-w.
- Chicago author-date (all authors)
- Claes, Katrien, Dries Van Herpe, Robin Vanluchene, Charlotte Roels, Berre Van Moer, Elise Wyseure, Kristof Vandewalle, Hannah Eeckhaut, Semiramis Yilmaz, Sandrine Vanmarcke, Erhan Citak, Daria Fijalkowska, Hendrik Grootaert, Chiara Lonigro, Leander Meuris, Gitte Michielsen, Justine Naessens, Loes van Schie, Riet De Rycke, Michiel De Bruyne, Peter Borghgraef, and Nico Callewaert. 2024. “OPENPichia : Licence-Free Komagataella Phaffii Chassis Strains and Toolkit for Protein Expression.” NATURE MICROBIOLOGY 9 (3): 864–876. doi:10.1038/s41564-023-01574-w.
- Vancouver
- 1.Claes K, Van Herpe D, Vanluchene R, Roels C, Van Moer B, Wyseure E, et al. OPENPichia : licence-free Komagataella phaffii chassis strains and toolkit for protein expression. NATURE MICROBIOLOGY. 2024;9(3):864–76.
- IEEE
- [1]K. Claes et al., “OPENPichia : licence-free Komagataella phaffii chassis strains and toolkit for protein expression,” NATURE MICROBIOLOGY, vol. 9, no. 3, pp. 864–876, 2024.
@article{01JJ1KWX4ZK4A2994N8948N8QP,
abstract = {{The industrial yeast Komagataella phaffii (formerly named Pichia pastoris) is commonly used to synthesize recombinant proteins, many of which are used as human therapeutics or in food. However, the basic strain, named NRRL Y-11430, from which all commercial hosts are derived, is not available without restrictions on its use. Comparative genome sequencing leaves little doubt that NRRL Y-11430 is derived from a K. phaffii type strain deposited in the UC Davis Phaff Yeast Strain Collection in 1954. We analysed four equivalent type strains in several culture collections and identified the NCYC 2543 strain, from which we started to develop an open-access Pichia chassis strain that anyone can use to produce recombinant proteins to industry standards. NRRL Y-11430 is readily transformable, which we found to be due to a HOC1 open-reading-frame truncation that alters cell-wall mannan. We introduced the HOC1 open-reading-frame truncation into NCYC 2543, which increased the transformability and improved secretion of some but not all of our tested proteins. We provide our genome-sequenced type strain, the hoc1(tr) derivative that we named OPENPichia as well as a synthetic, modular expression vector toolkit under liberal end-user distribution licences as an unencumbered OPENPichia resource for the microbial biotechnology community.}},
author = {{Claes, Katrien and Van Herpe, Dries and Vanluchene, Robin and Roels, Charlotte and Van Moer, Berre and Wyseure, Elise and Vandewalle, Kristof and Eeckhaut, Hannah and Yilmaz, Semiramis and Vanmarcke, Sandrine and Citak, Erhan and Fijalkowska, Daria and Grootaert, Hendrik and Lonigro, Chiara and Meuris, Leander and Michielsen, Gitte and Naessens, Justine and van Schie, Loes and De Rycke, Riet and De Bruyne, Michiel and Borghgraef, Peter and Callewaert, Nico}},
issn = {{2058-5276}},
journal = {{NATURE MICROBIOLOGY}},
keywords = {{PICHIA-PASTORIS,MICROBIAL-METABOLISM,YEAST,SEQUENCE,METHANOL}},
language = {{eng}},
number = {{3}},
pages = {{864--876}},
title = {{OPENPichia : licence-free Komagataella phaffii chassis strains and toolkit for protein expression}},
url = {{http://doi.org/10.1038/s41564-023-01574-w}},
volume = {{9}},
year = {{2024}},
}
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