The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure
- Author
- Lei Ding, Maxime J. Laurent, Thomas Milhiet, Stijn Aesaert (UGent) , Maria Van Lijsebettens (UGent) , Laurens Pauwels (UGent) , Hilde Nelissen (UGent) , Dirk Inzé (UGent) and François Chaumont
- Organization
- Project
- Abstract
- The plasma membrane aquaporin ZmPIP1;6 is expressed in maize stomatal complexes, with higher expression during the day than at night. To elucidate the role of ZmPIP1;6 in gas exchange and stomatal movement, it was expressed in maize (inbred line B104) under the control of p35S promoter (OE) or its native promoter fused with mYFP cDNA (mYFP-ZmPIP1;6). In stomatal complexes of the leaf mature zone, mYFP-ZmPIP1;6 showed higher expression in subsidiary cells than in guard cells, with light and dark treatments influencing its subcellular localization. Notably, ZmPIP1;6 internalization increased in dark conditions versus light. Stomatal opening was greater in ZmPIP1;6 OE than in wild type (WT), while closure exhibited greater sensitivity to elevated CO2 concentration or ABA treatment. Our finding revealed that reactive oxygen species (H2O2) was involved in ABA-induced stomatal closure, while ZmPIP1;6 was unable to facilitate H2O2 diffusion when expressed in yeast. Finally, ZmPIP1;6 OE and mYFP-ZmPIP1;6 transgenic plants exhibited higher abaxial stomatal density than WT. Overall, these results indicate that ZmPIP1;6 plays important roles in stomatal opening and CO2- and ABA-induced stomatal closure.
- Keywords
- ABA signaling, CO2 signaling, H2O2, Maize stomata, aquaporin, stomatal movement, PLASMA-MEMBRANE AQUAPORINS, ABSCISIC-ACID, ARABIDOPSIS-THALIANA, INTRINSIC PROTEINS, GROWTH-RATE, EXPRESSION, DROUGHT, CHANNEL, DIFFUSION, GENE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JHFEVKCKFMDTY2NAWZGKCWAQ
- MLA
- Ding, Lei, et al. “The Maize Aquaporin ZmPIP1;6 Enhances Stomatal Opening and CO2- and ABA-Induced Stomatal Closure.” JOURNAL OF EXPERIMENTAL BOTANY, edited by Tracy Lawson, vol. 76, no. 10, 2025, pp. 2832–45, doi:10.1093/jxb/erae500.
- APA
- Ding, L., Laurent, M. J., Milhiet, T., Aesaert, S., Van Lijsebettens, M., Pauwels, L., … Chaumont, F. (2025). The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure. JOURNAL OF EXPERIMENTAL BOTANY, 76(10), 2832–2845. https://doi.org/10.1093/jxb/erae500
- Chicago author-date
- Ding, Lei, Maxime J. Laurent, Thomas Milhiet, Stijn Aesaert, Maria Van Lijsebettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, and François Chaumont. 2025. “The Maize Aquaporin ZmPIP1;6 Enhances Stomatal Opening and CO2- and ABA-Induced Stomatal Closure.” Edited by Tracy Lawson. JOURNAL OF EXPERIMENTAL BOTANY 76 (10): 2832–45. https://doi.org/10.1093/jxb/erae500.
- Chicago author-date (all authors)
- Ding, Lei, Maxime J. Laurent, Thomas Milhiet, Stijn Aesaert, Maria Van Lijsebettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, and François Chaumont. 2025. “The Maize Aquaporin ZmPIP1;6 Enhances Stomatal Opening and CO2- and ABA-Induced Stomatal Closure.” Ed by. Tracy Lawson. JOURNAL OF EXPERIMENTAL BOTANY 76 (10): 2832–2845. doi:10.1093/jxb/erae500.
- Vancouver
- 1.Ding L, Laurent MJ, Milhiet T, Aesaert S, Van Lijsebettens M, Pauwels L, et al. The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure. Lawson T, editor. JOURNAL OF EXPERIMENTAL BOTANY. 2025;76(10):2832–45.
- IEEE
- [1]L. Ding et al., “The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 76, no. 10, pp. 2832–2845, 2025.
@article{01JHFEVKCKFMDTY2NAWZGKCWAQ,
abstract = {{The plasma membrane aquaporin ZmPIP1;6 is expressed in maize stomatal complexes, with higher expression during the day than at night. To elucidate the role of ZmPIP1;6 in gas exchange and stomatal movement, it was expressed in maize (inbred line B104) under the control of p35S promoter (OE) or its native promoter fused with mYFP cDNA (mYFP-ZmPIP1;6). In stomatal complexes of the leaf mature zone, mYFP-ZmPIP1;6 showed higher expression in subsidiary cells than in guard cells, with light and dark treatments influencing its subcellular localization. Notably, ZmPIP1;6 internalization increased in dark conditions versus light. Stomatal opening was greater in ZmPIP1;6 OE than in wild type (WT), while closure exhibited greater sensitivity to elevated CO2 concentration or ABA treatment. Our finding revealed that reactive oxygen species (H2O2) was involved in ABA-induced stomatal closure, while ZmPIP1;6 was unable to facilitate H2O2 diffusion when expressed in yeast. Finally, ZmPIP1;6 OE and mYFP-ZmPIP1;6 transgenic plants exhibited higher abaxial stomatal density than WT. Overall, these results indicate that ZmPIP1;6 plays important roles in stomatal opening and CO2- and ABA-induced stomatal closure.}},
author = {{Ding, Lei and Laurent, Maxime J. and Milhiet, Thomas and Aesaert, Stijn and Van Lijsebettens, Maria and Pauwels, Laurens and Nelissen, Hilde and Inzé, Dirk and Chaumont, François}},
editor = {{Lawson, Tracy}},
issn = {{0022-0957}},
journal = {{JOURNAL OF EXPERIMENTAL BOTANY}},
keywords = {{ABA signaling,CO2 signaling,H2O2,Maize stomata,aquaporin,stomatal movement,PLASMA-MEMBRANE AQUAPORINS,ABSCISIC-ACID,ARABIDOPSIS-THALIANA,INTRINSIC PROTEINS,GROWTH-RATE,EXPRESSION,DROUGHT,CHANNEL,DIFFUSION,GENE}},
language = {{eng}},
number = {{10}},
pages = {{2832--2845}},
title = {{The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure}},
url = {{http://doi.org/10.1093/jxb/erae500}},
volume = {{76}},
year = {{2025}},
}
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