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Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes

Klaartje Broothaers (UGent) , Osvaldo Américo Bogado Pascottini (UGent) , Mohamed Hedia (UGent) , Daniel Angel Velez (UGent) , Tine De Coster (UGent) , Sofie Peere (UGent) , Ellen Polfliet (UGent) , Emma Van den Branden (UGent) , Jan Govaere (UGent) , Ann Van Soom (UGent) , et al.
(2025) THERIOGENOLOGY. 233. p.64-69
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Abstract
Previous studies in the horse highlight the potential benefit of prolonged in vitro maturation (IVM) (34 h) compared to short IVM (24 h) with or without prior oocyte holding, but little is known about the optimal IVM duration within this interval. To determine the effect of oocyte holding and duration of IVM ranged between 28 and 34 h on nuclear maturation, cleavage, blastocyst formation, and pregnancy rates, a retrospective study was performed in an equine clinical OPU-ICSI setting. The study included data of 2114 aspirated oocytes from 201 OPU-ICSI sessions. Duration of IVM was divided in three different time windows using quartiles, with 465 oocytes (22.0 %) between 28 and 30 h (first quartile), 1078 oocytes (51.0 %) >30 and 31.7 h (second and third quartiles), and 571 oocytes (27.0 %) >31.7 and 34 h (fourth quartile). Using logistic regression models, the effect of duration of IVM with and without holding was tested on nuclear maturation, cleavage, blastocyst, and pregnancy rates. The three IVM intervals did not show differences in nuclear maturation (respectively 64.5 +/- 0.48 %, 65.7 +/- 0.47 %, and 67.3 +/- 0.47 %), cleavage (respectively 59.7 +/- 0.49 %, 58.5 +/- 0.49 %, and 64.8 +/- 0.48 %), blastocyst (respectively 17.5 +/- 0.38 %, 19.0 +/- 0.39 %, and 20.8 +/- 0.41 %) nor pregnancy rates (respectively 65.4 +/- 0.49 %, 70.3 +/- 0.46 %, and 74.2 % +/- 0.44) (P >= 0.38). Oocyte holding prior to IVM did not affect the results either (P >= 0.15). In conclusion, oocyte holding and IVM duration between 28 and 34h do not significantly affect outcomes, allowing flexibility in the planning of clinical OPU-ICSI in horses.
Keywords
Horse, Cleavage, IVM, Blastocyst, Timing, SPERM INJECTION ICSI, BLASTOCYST DEVELOPMENT, GERMINAL VESICLE, CHROMATIN, SEMEN

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MLA
Broothaers, Klaartje, et al. “Oocyte Holding and in Vitro Maturation Duration between 28 and 34 Hours Do Not Affect Equine OPU-ICSI Outcomes.” THERIOGENOLOGY, vol. 233, 2025, pp. 64–69, doi:10.1016/j.theriogenology.2024.11.019.
APA
Broothaers, K., Bogado Pascottini, O. A., Hedia, M., Angel Velez, D., De Coster, T., Peere, S., … Smits, K. (2025). Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes. THERIOGENOLOGY, 233, 64–69. https://doi.org/10.1016/j.theriogenology.2024.11.019
Chicago author-date
Broothaers, Klaartje, Osvaldo Américo Bogado Pascottini, Mohamed Hedia, Daniel Angel Velez, Tine De Coster, Sofie Peere, Ellen Polfliet, et al. 2025. “Oocyte Holding and in Vitro Maturation Duration between 28 and 34 Hours Do Not Affect Equine OPU-ICSI Outcomes.” THERIOGENOLOGY 233: 64–69. https://doi.org/10.1016/j.theriogenology.2024.11.019.
Chicago author-date (all authors)
Broothaers, Klaartje, Osvaldo Américo Bogado Pascottini, Mohamed Hedia, Daniel Angel Velez, Tine De Coster, Sofie Peere, Ellen Polfliet, Emma Van den Branden, Jan Govaere, Ann Van Soom, and Katrien Smits. 2025. “Oocyte Holding and in Vitro Maturation Duration between 28 and 34 Hours Do Not Affect Equine OPU-ICSI Outcomes.” THERIOGENOLOGY 233: 64–69. doi:10.1016/j.theriogenology.2024.11.019.
Vancouver
1.
Broothaers K, Bogado Pascottini OA, Hedia M, Angel Velez D, De Coster T, Peere S, et al. Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes. THERIOGENOLOGY. 2025;233:64–9.
IEEE
[1]
K. Broothaers et al., “Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes,” THERIOGENOLOGY, vol. 233, pp. 64–69, 2025.
@article{01JHFE1VVP3WP2K49N61Y0BP4J,
  abstract     = {{Previous studies in the horse highlight the potential benefit of prolonged in vitro maturation (IVM) (34 h) compared to short IVM (24 h) with or without prior oocyte holding, but little is known about the optimal IVM duration within this interval. To determine the effect of oocyte holding and duration of IVM ranged between 28 and 34 h on nuclear maturation, cleavage, blastocyst formation, and pregnancy rates, a retrospective study was performed in an equine clinical OPU-ICSI setting. The study included data of 2114 aspirated oocytes from 201 OPU-ICSI sessions. Duration of IVM was divided in three different time windows using quartiles, with 465 oocytes (22.0 %) between 28 and 30 h (first quartile), 1078 oocytes (51.0 %) >30 and 31.7 h (second and third quartiles), and 571 oocytes (27.0 %) >31.7 and 34 h (fourth quartile). Using logistic regression models, the effect of duration of IVM with and without holding was tested on nuclear maturation, cleavage, blastocyst, and pregnancy rates. The three IVM intervals did not show differences in nuclear maturation (respectively 64.5 +/- 0.48 %, 65.7 +/- 0.47 %, and 67.3 +/- 0.47 %), cleavage (respectively 59.7 +/- 0.49 %, 58.5 +/- 0.49 %, and 64.8 +/- 0.48 %), blastocyst (respectively 17.5 +/- 0.38 %, 19.0 +/- 0.39 %, and 20.8 +/- 0.41 %) nor pregnancy rates (respectively 65.4 +/- 0.49 %, 70.3 +/- 0.46 %, and 74.2 % +/- 0.44) (P >= 0.38). Oocyte holding prior to IVM did not affect the results either (P >= 0.15). In conclusion, oocyte holding and IVM duration between 28 and 34h do not significantly affect outcomes, allowing flexibility in the planning of clinical OPU-ICSI in horses.}},
  author       = {{Broothaers, Klaartje and Bogado Pascottini, Osvaldo Américo and Hedia, Mohamed and Angel Velez, Daniel and De Coster, Tine and Peere, Sofie and Polfliet, Ellen and Van den Branden, Emma and Govaere, Jan and Van Soom, Ann and Smits, Katrien}},
  issn         = {{0093-691X}},
  journal      = {{THERIOGENOLOGY}},
  keywords     = {{Horse,Cleavage,IVM,Blastocyst,Timing,SPERM INJECTION ICSI,BLASTOCYST DEVELOPMENT,GERMINAL VESICLE,CHROMATIN,SEMEN}},
  language     = {{eng}},
  pages        = {{64--69}},
  title        = {{Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes}},
  url          = {{http://doi.org/10.1016/j.theriogenology.2024.11.019}},
  volume       = {{233}},
  year         = {{2025}},
}
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