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Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis

Guan XueFeng (UGent) , Yuan Fan (UGent) , Rani Six (UGent) , Camilla Benedetti (UGent) , Annelies Raes (UGent) , Andrea Fernández Montoro (UGent) , Xiaole Cui (UGent) , Nima AzariDolatabad, Ann Van Soom (UGent) , Krishna Chaitanya Pavani (UGent) , et al.
(2024) FRONTIERS IN GENETICS. 15.
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Abstract
Extracellular vesicles (EVs) contain microRNAs (miRNAs), which are important regulators of embryonic development. Nevertheless, little is known about the precise molecular processes controlling blastocyst development and quality. In a previous study, we identified bta-miR-665 as one of the miRNAs more abundantly present in extracellular vesicles of embryo-conditioned culture media of blastocysts compared to degenerate ones. Here, we investigated the effect and regulatory roles of bta-miR-665 in blastocyst development by supplementation of bta-miR-665 mimics or inhibitors to the culture media. Supplementation of bta-miR-665 mimics improved cleavage and blastocyst rate (P < 0.01), and blastocyst quality as indicated by increased inner cell mass rates and reduced apoptotic cell ratios (P < 0.01). Furthermore, supplementation of bta-miR-665 inhibitors had the opposite effect on these phenotypes. Low input transcriptome analysis and RT-qPCR revealed that bta-miR-665 acts on genes linked to microtubule formation and apoptosis/cell proliferation. These insights not only elucidate the important role of bta-miR-665 in embryo development, but also underscore its potential in improving reproductive efficiency in bovine embryo culture.
Keywords
MicroRNAs, blastocyst formation, embryo development, microtubules, cell proliferation, EMBRYO QUALITY, PROTEIN-KINASE, MICRORNA, TRANSITION, EXPRESSION, MATURATION, ROLES

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MLA
XueFeng, Guan, et al. “Bta-MiR-665 Improves Bovine Blastocyst Development through Its Influence on Microtubule Dynamics and Apoptosis.” FRONTIERS IN GENETICS, vol. 15, 2024, doi:10.3389/fgene.2024.1437695.
APA
XueFeng, G., Fan, Y., Six, R., Benedetti, C., Raes, A., Fernández Montoro, A., … Peelman, L. (2024). Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis. FRONTIERS IN GENETICS, 15. https://doi.org/10.3389/fgene.2024.1437695
Chicago author-date
XueFeng, Guan, Yuan Fan, Rani Six, Camilla Benedetti, Annelies Raes, Andrea Fernández Montoro, Xiaole Cui, et al. 2024. “Bta-MiR-665 Improves Bovine Blastocyst Development through Its Influence on Microtubule Dynamics and Apoptosis.” FRONTIERS IN GENETICS 15. https://doi.org/10.3389/fgene.2024.1437695.
Chicago author-date (all authors)
XueFeng, Guan, Yuan Fan, Rani Six, Camilla Benedetti, Annelies Raes, Andrea Fernández Montoro, Xiaole Cui, Nima AzariDolatabad, Ann Van Soom, Krishna Chaitanya Pavani, and Luc Peelman. 2024. “Bta-MiR-665 Improves Bovine Blastocyst Development through Its Influence on Microtubule Dynamics and Apoptosis.” FRONTIERS IN GENETICS 15. doi:10.3389/fgene.2024.1437695.
Vancouver
1.
XueFeng G, Fan Y, Six R, Benedetti C, Raes A, Fernández Montoro A, et al. Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis. FRONTIERS IN GENETICS. 2024;15.
IEEE
[1]
G. XueFeng et al., “Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis,” FRONTIERS IN GENETICS, vol. 15, 2024.
@article{01JH0D3NH9Z4S0FZRTHKFXGQTQ,
  abstract     = {{Extracellular vesicles (EVs) contain microRNAs (miRNAs), which are important regulators of embryonic development. Nevertheless, little is known about the precise molecular processes controlling blastocyst development and quality. In a previous study, we identified bta-miR-665 as one of the miRNAs more abundantly present in extracellular vesicles of embryo-conditioned culture media of blastocysts compared to degenerate ones. Here, we investigated the effect and regulatory roles of bta-miR-665 in blastocyst development by supplementation of bta-miR-665 mimics or inhibitors to the culture media. Supplementation of bta-miR-665 mimics improved cleavage and blastocyst rate (P < 0.01), and blastocyst quality as indicated by increased inner cell mass rates and reduced apoptotic cell ratios (P < 0.01). Furthermore, supplementation of bta-miR-665 inhibitors had the opposite effect on these phenotypes. Low input transcriptome analysis and RT-qPCR revealed that bta-miR-665 acts on genes linked to microtubule formation and apoptosis/cell proliferation. These insights not only elucidate the important role of bta-miR-665 in embryo development, but also underscore its potential in improving reproductive efficiency in bovine embryo culture.}},
  articleno    = {{1437695}},
  author       = {{XueFeng, Guan and Fan, Yuan and Six, Rani and Benedetti, Camilla and Raes, Annelies and Fernández Montoro, Andrea and Cui, Xiaole and AzariDolatabad, Nima and Van Soom, Ann and Pavani, Krishna Chaitanya and Peelman, Luc}},
  issn         = {{1664-8021}},
  journal      = {{FRONTIERS IN GENETICS}},
  keywords     = {{MicroRNAs,blastocyst formation,embryo development,microtubules,cell proliferation,EMBRYO QUALITY,PROTEIN-KINASE,MICRORNA,TRANSITION,EXPRESSION,MATURATION,ROLES}},
  language     = {{eng}},
  pages        = {{10}},
  title        = {{Bta-miR-665 improves bovine blastocyst development through its influence on microtubule dynamics and apoptosis}},
  url          = {{http://doi.org/10.3389/fgene.2024.1437695}},
  volume       = {{15}},
  year         = {{2024}},
}
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