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Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS)

Wilhelmina Radstake, Alessio Parisi, Silvana Ferreira Da Silva Miranda (UGent) , Kiran Gautam, Randy Vermeesen, Emil Rehnberg (UGent) , Kevin Tabury, Rob Coppes, Marc-Jan van Goethem, Sytze Brandenburg, et al.
(2024) SCIENTIFIC REPORTS. 14(1).
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Abstract
Without the protective shielding of Earth's atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities.
Keywords
Ionizing radiation, Iron ions, Carbon ions, Protons, Cortisol, Fibroblast, DNA damage, DNA repair, STRESS, REPAIR, PROLIFERATION, DYNAMICS, LESIONS

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MLA
Radstake, Wilhelmina, et al. “Radiation-Induced DNA Double-Strand Breaks in Cortisol Exposed Fibroblasts as Quantified with the Novel Foci-Integrated Damage Complexity Score (FIDCS).” SCIENTIFIC REPORTS, vol. 14, no. 1, 2024, doi:10.1038/s41598-024-60912-y.
APA
Radstake, W., Parisi, A., Ferreira Da Silva Miranda, S., Gautam, K., Vermeesen, R., Rehnberg, E., … Baatout, S. (2024). Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS). SCIENTIFIC REPORTS, 14(1). https://doi.org/10.1038/s41598-024-60912-y
Chicago author-date
Radstake, Wilhelmina, Alessio Parisi, Silvana Ferreira Da Silva Miranda, Kiran Gautam, Randy Vermeesen, Emil Rehnberg, Kevin Tabury, et al. 2024. “Radiation-Induced DNA Double-Strand Breaks in Cortisol Exposed Fibroblasts as Quantified with the Novel Foci-Integrated Damage Complexity Score (FIDCS).” SCIENTIFIC REPORTS 14 (1). https://doi.org/10.1038/s41598-024-60912-y.
Chicago author-date (all authors)
Radstake, Wilhelmina, Alessio Parisi, Silvana Ferreira Da Silva Miranda, Kiran Gautam, Randy Vermeesen, Emil Rehnberg, Kevin Tabury, Rob Coppes, Marc-Jan van Goethem, Sytze Brandenburg, Ulrich Weber, Claudia Fournier, Marco Durante, Bjorn Baselet, and Sarah Baatout. 2024. “Radiation-Induced DNA Double-Strand Breaks in Cortisol Exposed Fibroblasts as Quantified with the Novel Foci-Integrated Damage Complexity Score (FIDCS).” SCIENTIFIC REPORTS 14 (1). doi:10.1038/s41598-024-60912-y.
Vancouver
1.
Radstake W, Parisi A, Ferreira Da Silva Miranda S, Gautam K, Vermeesen R, Rehnberg E, et al. Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS). SCIENTIFIC REPORTS. 2024;14(1).
IEEE
[1]
W. Radstake et al., “Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS),” SCIENTIFIC REPORTS, vol. 14, no. 1, 2024.
@article{01JG2BZXTJS9G3HE1KXEK4PVMD,
  abstract     = {{Without the protective shielding of Earth's atmosphere, astronauts face higher doses of ionizing radiation in space, causing serious health concerns. Highly charged and high energy (HZE) particles are particularly effective in causing complex and difficult-to-repair DNA double-strand breaks compared to low linear energy transfer. Additionally, chronic cortisol exposure during spaceflight raises further concerns, although its specific impact on DNA damage and repair remains unknown. This study explorers the effect of different radiation qualities (photons, protons, carbon, and iron ions) on the DNA damage and repair of cortisol-conditioned primary human dermal fibroblasts. Besides, we introduce a new measure, the Foci-Integrated Damage Complexity Score (FIDCS), to assess DNA damage complexity by analyzing focus area and fluorescent intensity. Our results show that the FIDCS captured the DNA damage induced by different radiation qualities better than counting the number of foci, as traditionally done. Besides, using this measure, we were able to identify differences in DNA damage between cortisol-exposed cells and controls. This suggests that, besides measuring the total number of foci, considering the complexity of the DNA damage by means of the FIDCS can provide additional and, in our case, improved information when comparing different radiation qualities.}},
  articleno    = {{10400}},
  author       = {{Radstake, Wilhelmina and Parisi, Alessio and Ferreira Da Silva Miranda, Silvana and Gautam, Kiran and Vermeesen, Randy and Rehnberg, Emil and Tabury, Kevin and Coppes, Rob and van Goethem, Marc-Jan and Brandenburg, Sytze and Weber, Ulrich and Fournier, Claudia and Durante, Marco and Baselet, Bjorn and Baatout, Sarah}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{Ionizing radiation,Iron ions,Carbon ions,Protons,Cortisol,Fibroblast,DNA damage,DNA repair,STRESS,REPAIR,PROLIFERATION,DYNAMICS,LESIONS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{15}},
  title        = {{Radiation-induced DNA double-strand breaks in cortisol exposed fibroblasts as quantified with the novel foci-integrated damage complexity score (FIDCS)}},
  url          = {{http://doi.org/10.1038/s41598-024-60912-y}},
  volume       = {{14}},
  year         = {{2024}},
}
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