ZEB2 drives intra-tumor heterogeneity and skin squamous cell carcinoma formation with distinct EMP transition states
- Author
- Jeroen Verstappe (UGent) , Nicolas Skrypek (UGent) , Jordy De Coninck (UGent) , Bieke Soen (UGent) , Joachim Taminau (UGent) , Marianthi Tatari, Kenneth Bruneel, Nele Loret (UGent) , Kato De Clercq (UGent) , Caroline Van den Broecke (UGent) , Koen Van de Vijver (UGent) , Jo Van Dorpe (UGent) , Jody Haigh, Bram De Craene (UGent) , Steven Goossens (UGent) , Niels Vandamme (UGent) and Geert Berx (UGent)
- Organization
- Project
-
- Targeting the Tumor Microenvironment in Colorectal Peritoneal Metastases
- Preclinical validation of novel anti-EMT therapy for malignant triple negative breast cancer
- Defining the role of ZEB1 in tumour initiation and progression of basal-like breast cancer
- Dissecting stromal-cancer cell interactions at the perivascular niche to detect and intercept the early metastatic process.
- Unravelling EMT-controlled aetiology and chemotherapy resistance of ovarian carcinosarcoma
- Abstract
- About 5% of patients with cutaneous squamous cell carcinoma (cSCC) have a poor prognosis which is associated with a loss of tumor differentiation, invasion and metastasis, all of which are linked to the process of epithelial-to-mesenchymal plasticity (EMP). Here, we showed that the EMP-associated transcription factor ZEB2 drives cSCC heterogeneity which resembles biphasic carcinosarcoma-like tumors. Single cell RNA sequencing revealed distinct subpopulations ranging from fully epithelial (E) to intermediate (EM) to fully mesenchymal (M), associated with the gradual loss of cell surface markers EPCAM, CDH1, ITGB4, and CD200. Mesenchymal features were associated with a higher metastatic capacity and anoikis resistance, yet this comes with a sensitivity toward TNF-induced cell death. Altogether we provide insights in cSCC heterogeneity and modes to target mesenchymal-metastasis inducing cells.
- Keywords
- EPITHELIAL-MESENCHYMAL TRANSITION, CANCER, EXPRESSION, GUIDELINES, RESISTANCE, DEATH
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JFCFKW3H48NKM1CK9NYJZ7TE
- MLA
- Verstappe, Jeroen, et al. “ZEB2 Drives Intra-Tumor Heterogeneity and Skin Squamous Cell Carcinoma Formation with Distinct EMP Transition States.” ISCIENCE, vol. 27, no. 11, 2024, doi:10.1016/j.isci.2024.111169.
- APA
- Verstappe, J., Skrypek, N., De Coninck, J., Soen, B., Taminau, J., Tatari, M., … Berx, G. (2024). ZEB2 drives intra-tumor heterogeneity and skin squamous cell carcinoma formation with distinct EMP transition states. ISCIENCE, 27(11). https://doi.org/10.1016/j.isci.2024.111169
- Chicago author-date
- Verstappe, Jeroen, Nicolas Skrypek, Jordy De Coninck, Bieke Soen, Joachim Taminau, Marianthi Tatari, Kenneth Bruneel, et al. 2024. “ZEB2 Drives Intra-Tumor Heterogeneity and Skin Squamous Cell Carcinoma Formation with Distinct EMP Transition States.” ISCIENCE 27 (11). https://doi.org/10.1016/j.isci.2024.111169.
- Chicago author-date (all authors)
- Verstappe, Jeroen, Nicolas Skrypek, Jordy De Coninck, Bieke Soen, Joachim Taminau, Marianthi Tatari, Kenneth Bruneel, Nele Loret, Kato De Clercq, Caroline Van den Broecke, Koen Van de Vijver, Jo Van Dorpe, Jody Haigh, Bram De Craene, Steven Goossens, Niels Vandamme, and Geert Berx. 2024. “ZEB2 Drives Intra-Tumor Heterogeneity and Skin Squamous Cell Carcinoma Formation with Distinct EMP Transition States.” ISCIENCE 27 (11). doi:10.1016/j.isci.2024.111169.
- Vancouver
- 1.Verstappe J, Skrypek N, De Coninck J, Soen B, Taminau J, Tatari M, et al. ZEB2 drives intra-tumor heterogeneity and skin squamous cell carcinoma formation with distinct EMP transition states. ISCIENCE. 2024;27(11).
- IEEE
- [1]J. Verstappe et al., “ZEB2 drives intra-tumor heterogeneity and skin squamous cell carcinoma formation with distinct EMP transition states,” ISCIENCE, vol. 27, no. 11, 2024.
@article{01JFCFKW3H48NKM1CK9NYJZ7TE,
abstract = {{About 5% of patients with cutaneous squamous cell carcinoma (cSCC) have a poor prognosis which is associated with a loss of tumor differentiation, invasion and metastasis, all of which are linked to the process of epithelial-to-mesenchymal plasticity (EMP). Here, we showed that the EMP-associated transcription factor ZEB2 drives cSCC heterogeneity which resembles biphasic carcinosarcoma-like tumors. Single cell RNA sequencing revealed distinct subpopulations ranging from fully epithelial (E) to intermediate (EM) to fully mesenchymal (M), associated with the gradual loss of cell surface markers EPCAM, CDH1, ITGB4, and CD200. Mesenchymal features were associated with a higher metastatic capacity and anoikis resistance, yet this comes with a sensitivity toward TNF-induced cell death. Altogether we provide insights in cSCC heterogeneity and modes to target mesenchymal-metastasis inducing cells.}},
articleno = {{111169}},
author = {{Verstappe, Jeroen and Skrypek, Nicolas and De Coninck, Jordy and Soen, Bieke and Taminau, Joachim and Tatari, Marianthi and Bruneel, Kenneth and Loret, Nele and De Clercq, Kato and Van den Broecke, Caroline and Van de Vijver, Koen and Van Dorpe, Jo and Haigh, Jody and De Craene, Bram and Goossens, Steven and Vandamme, Niels and Berx, Geert}},
issn = {{2589-0042}},
journal = {{ISCIENCE}},
keywords = {{EPITHELIAL-MESENCHYMAL TRANSITION,CANCER,EXPRESSION,GUIDELINES,RESISTANCE,DEATH}},
language = {{eng}},
number = {{11}},
pages = {{18}},
title = {{ZEB2 drives intra-tumor heterogeneity and skin squamous cell carcinoma formation with distinct EMP transition states}},
url = {{http://doi.org/10.1016/j.isci.2024.111169}},
volume = {{27}},
year = {{2024}},
}
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