Functionally modified graphene oxide as an alternative nanovehicle for enhanced dsRNA delivery in improving RNAi-based insect pest control
- Author
- Qi Xue, jiangjie Li (UGent) , Sven Vereecken (UGent) , Qiqiong Li, Zijian Zhi, Peter Dubruel (UGent) , Nji Tizi Clauvis Taning (UGent) and Kristof De Schutter (UGent)
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- Project
- Abstract
- RNA interference (RNAi) has shown substantial promise as a sustainable pest management solution. However, the efficacy of RNAi-based insecticides heavily relies on advanced nanocarrier-mediated delivery systems. In this study, we modified raw graphene oxide into positively charged nanocarriers (GONs) tailored to bind with double-stranded RNA (dsRNA). The resulting GONs@dsRNA complexes demonstrated a small particle size (106 nm) and maintained stability under various conditions, including insect gut extracts, extreme pH, and extreme temperature. Furthermore, GONs efficiently transported dsRNA molecules into Drosophila S2 cells and Lepidoptera Sf9 cells, leading to an enhanced target transcript knockdown. Targeting the vacuolar ATPase gene, vha26, induced significant mortality and target transcript knockdown in D. suzukii adults but not in S. exigua. Finally, GONs@dsRNA complexes exhibited negligible cytotoxicity at both the cellular and organismal levels. This study demonstrates the potential of GONs as a biosafe nanovehicle for efficient dsRNA delivery into insects, presenting an alternative strategy for advancing RNAi applications in fundamental studies and pest control.
- Keywords
- RNA interference (RNAi), graphene oxide nanocarriers, cytotoxicity, pest control, DROSOPHILA-SUZUKII, SPODOPTERA-EXIGUA, IN-VIVO, INTERFERENCE, GENE, INVASION, BIOLOGY, LARVAL, CELLS
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JENCKK055DXQ8439Z6PMGB41
- MLA
- Xue, Qi, et al. “Functionally Modified Graphene Oxide as an Alternative Nanovehicle for Enhanced DsRNA Delivery in Improving RNAi-Based Insect Pest Control.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 72, no. 41, 2024, pp. 22512–23, doi:10.1021/acs.jafc.4c05215.
- APA
- Xue, Q., Li, jiangjie, Vereecken, S., Li, Q., Zhi, Z., Dubruel, P., … De Schutter, K. (2024). Functionally modified graphene oxide as an alternative nanovehicle for enhanced dsRNA delivery in improving RNAi-based insect pest control. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 72(41), 22512–22523. https://doi.org/10.1021/acs.jafc.4c05215
- Chicago author-date
- Xue, Qi, jiangjie Li, Sven Vereecken, Qiqiong Li, Zijian Zhi, Peter Dubruel, Nji Tizi Clauvis Taning, and Kristof De Schutter. 2024. “Functionally Modified Graphene Oxide as an Alternative Nanovehicle for Enhanced DsRNA Delivery in Improving RNAi-Based Insect Pest Control.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 (41): 22512–23. https://doi.org/10.1021/acs.jafc.4c05215.
- Chicago author-date (all authors)
- Xue, Qi, jiangjie Li, Sven Vereecken, Qiqiong Li, Zijian Zhi, Peter Dubruel, Nji Tizi Clauvis Taning, and Kristof De Schutter. 2024. “Functionally Modified Graphene Oxide as an Alternative Nanovehicle for Enhanced DsRNA Delivery in Improving RNAi-Based Insect Pest Control.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 (41): 22512–22523. doi:10.1021/acs.jafc.4c05215.
- Vancouver
- 1.Xue Q, Li jiangjie, Vereecken S, Li Q, Zhi Z, Dubruel P, et al. Functionally modified graphene oxide as an alternative nanovehicle for enhanced dsRNA delivery in improving RNAi-based insect pest control. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2024;72(41):22512–23.
- IEEE
- [1]Q. Xue et al., “Functionally modified graphene oxide as an alternative nanovehicle for enhanced dsRNA delivery in improving RNAi-based insect pest control,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 72, no. 41, pp. 22512–22523, 2024.
@article{01JENCKK055DXQ8439Z6PMGB41,
abstract = {{RNA interference (RNAi) has shown substantial promise as a sustainable pest management solution. However, the efficacy of RNAi-based insecticides heavily relies on advanced nanocarrier-mediated delivery systems. In this study, we modified raw graphene oxide into positively charged nanocarriers (GONs) tailored to bind with double-stranded RNA (dsRNA). The resulting GONs@dsRNA complexes demonstrated a small particle size (106 nm) and maintained stability under various conditions, including insect gut extracts, extreme pH, and extreme temperature. Furthermore, GONs efficiently transported dsRNA molecules into Drosophila S2 cells and Lepidoptera Sf9 cells, leading to an enhanced target transcript knockdown. Targeting the vacuolar ATPase gene, vha26, induced significant mortality and target transcript knockdown in D. suzukii adults but not in S. exigua. Finally, GONs@dsRNA complexes exhibited negligible cytotoxicity at both the cellular and organismal levels. This study demonstrates the potential of GONs as a biosafe nanovehicle for efficient dsRNA delivery into insects, presenting an alternative strategy for advancing RNAi applications in fundamental studies and pest control.}},
author = {{Xue, Qi and Li, jiangjie and Vereecken, Sven and Li, Qiqiong and Zhi, Zijian and Dubruel, Peter and Taning, Nji Tizi Clauvis and De Schutter, Kristof}},
issn = {{0021-8561}},
journal = {{JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}},
keywords = {{RNA interference (RNAi),graphene oxide nanocarriers,cytotoxicity,pest control,DROSOPHILA-SUZUKII,SPODOPTERA-EXIGUA,IN-VIVO,INTERFERENCE,GENE,INVASION,BIOLOGY,LARVAL,CELLS}},
language = {{eng}},
number = {{41}},
pages = {{22512--22523}},
title = {{Functionally modified graphene oxide as an alternative nanovehicle for enhanced dsRNA delivery in improving RNAi-based insect pest control}},
url = {{http://doi.org/10.1021/acs.jafc.4c05215}},
volume = {{72}},
year = {{2024}},
}
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