Alginate-CaCO3 hybrid colloidal hydrogel with tunable physicochemical properties for cell growth
- Author
- Lin Cao (UGent) , Joost Verduijn (UGent) , Louis Van der Meeren (UGent) , Yanqi Huang (UGent) , Laura Currás Vallejos, Andre Skirtach (UGent) and Bogdan Parakhonskiy (UGent)
- Organization
- Project
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- Nano-chemical treatment of wood for enhanced fungal and fire resistance
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- Triple punch against melanoma: photodynamic therapy, immunogenic cell death and photothermal ablation
- Harnessing tumor acidosis and immunogenic ferroptosis for an innovative treatment of peritoneal carcinomatosis with PUFA-loaded nanovesicles
- Biofabrication of a 3D bone-on-a-chip by inkjet printing for microgravity research
- “Trojan-horse” approach to biodegradable, food-based drug carriers with antibacterial properties.
- PASCell: parameter activated sorting of cells
- Abstract
- Biomaterials composed of food polysaccharides are of great interest for future biomedical applications due to their great biocompatibility, tunable mechanical properties, and complex architectural designs that play a crucial role in the modulation of cell adhesion and proliferation. In this work, a facile approach was designed to obtain novel 3D alginate-CaCO3 hybrid hydrogel particles in situ. Controlling the gel concentration from 3 to 20 mg & sdot;mL- 1 allows us to control the alginate-CaCO3 hydrogel particles' size and density (size variation from 1.86 to 2.34 mm and density from 1.22 to 1.29 mg/mm3). This variable also has a considerable influence on the mineralization process resulting in CaCO3 particles with varied sizes and amounts within the hydrogel beads. The measurements of Young's modulus showed that the inclusion of CaCO3 particles into the alginate hydrogel improved its mechanical properties, and Young's modulus of these hybrid hydrogel particles had a linear relationship with alginate content and hydrogel particle size. Cell experiments indicated that alginate-CaCO3 hybrid hydrogel particles can support osteoblastic cell proliferation and growth. In particular, the amount of hydroxyapatite deposition on the cell membrane significantly increased after the treatment of cells with hybrid hydrogel particles, up to 20 -fold. This work offers a strategy for constructing inorganic particle -doped polysaccharide hybrid hydrogel scaffolds that provide the potential to support cell growth.
- Keywords
- Alginate, Calcium carbonate, Hybrid hydrogel, Osteoblastic cell, Hydroxyapatite deposition, MECHANICAL-PROPERTIES, TISSUE, ADHESION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01JE6D8GAJ4VJMTAZQ6ZEWZC97
- MLA
- Cao, Lin, et al. “Alginate-CaCO3 Hybrid Colloidal Hydrogel with Tunable Physicochemical Properties for Cell Growth.” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 259, 2024, doi:10.1016/j.ijbiomac.2023.129069.
- APA
- Cao, L., Verduijn, J., Van der Meeren, L., Huang, Y., Currás Vallejos, L., Skirtach, A., & Parakhonskiy, B. (2024). Alginate-CaCO3 hybrid colloidal hydrogel with tunable physicochemical properties for cell growth. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 259. https://doi.org/10.1016/j.ijbiomac.2023.129069
- Chicago author-date
- Cao, Lin, Joost Verduijn, Louis Van der Meeren, Yanqi Huang, Laura Currás Vallejos, Andre Skirtach, and Bogdan Parakhonskiy. 2024. “Alginate-CaCO3 Hybrid Colloidal Hydrogel with Tunable Physicochemical Properties for Cell Growth.” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 259. https://doi.org/10.1016/j.ijbiomac.2023.129069.
- Chicago author-date (all authors)
- Cao, Lin, Joost Verduijn, Louis Van der Meeren, Yanqi Huang, Laura Currás Vallejos, Andre Skirtach, and Bogdan Parakhonskiy. 2024. “Alginate-CaCO3 Hybrid Colloidal Hydrogel with Tunable Physicochemical Properties for Cell Growth.” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 259. doi:10.1016/j.ijbiomac.2023.129069.
- Vancouver
- 1.Cao L, Verduijn J, Van der Meeren L, Huang Y, Currás Vallejos L, Skirtach A, et al. Alginate-CaCO3 hybrid colloidal hydrogel with tunable physicochemical properties for cell growth. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2024;259.
- IEEE
- [1]L. Cao et al., “Alginate-CaCO3 hybrid colloidal hydrogel with tunable physicochemical properties for cell growth,” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 259, 2024.
@article{01JE6D8GAJ4VJMTAZQ6ZEWZC97,
abstract = {{Biomaterials composed of food polysaccharides are of great interest for future biomedical applications due to their great biocompatibility, tunable mechanical properties, and complex architectural designs that play a crucial role in the modulation of cell adhesion and proliferation. In this work, a facile approach was designed to obtain novel 3D alginate-CaCO3 hybrid hydrogel particles in situ. Controlling the gel concentration from 3 to 20 mg & sdot;mL- 1 allows us to control the alginate-CaCO3 hydrogel particles' size and density (size variation from 1.86 to 2.34 mm and density from 1.22 to 1.29 mg/mm3). This variable also has a considerable influence on the mineralization process resulting in CaCO3 particles with varied sizes and amounts within the hydrogel beads. The measurements of Young's modulus showed that the inclusion of CaCO3 particles into the alginate hydrogel improved its mechanical properties, and Young's modulus of these hybrid hydrogel particles had a linear relationship with alginate content and hydrogel particle size. Cell experiments indicated that alginate-CaCO3 hybrid hydrogel particles can support osteoblastic cell proliferation and growth. In particular, the amount of hydroxyapatite deposition on the cell membrane significantly increased after the treatment of cells with hybrid hydrogel particles, up to 20 -fold. This work offers a strategy for constructing inorganic particle -doped polysaccharide hybrid hydrogel scaffolds that provide the potential to support cell growth.}},
articleno = {{129069}},
author = {{Cao, Lin and Verduijn, Joost and Van der Meeren, Louis and Huang, Yanqi and Currás Vallejos, Laura and Skirtach, Andre and Parakhonskiy, Bogdan}},
issn = {{0141-8130}},
journal = {{INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES}},
keywords = {{Alginate,Calcium carbonate,Hybrid hydrogel,Osteoblastic cell,Hydroxyapatite deposition,MECHANICAL-PROPERTIES,TISSUE,ADHESION}},
language = {{eng}},
pages = {{12}},
title = {{Alginate-CaCO3 hybrid colloidal hydrogel with tunable physicochemical properties for cell growth}},
url = {{http://doi.org/10.1016/j.ijbiomac.2023.129069}},
volume = {{259}},
year = {{2024}},
}
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