@article{01JE0VTPCZAB9N12DA72WNKA79,
  abstract     = {{Whole-Genome Sequencing has the potential to be an effective method for surveillance of foodborne diseases. This study aims to determine the genetic relatedness and prevalence of virulence-associated genes and antimicrobial resistance determinants in 135 Campylobacter jejuni, seven Campylobacter coli and three Campylobacter lari isolates from the poultry supply chain and a hospital in Spain. The isolates showed a wide genetic diversity between and within species with Clonal Complex 21 the most frequent lineage found. Among species, C. jejuni showed the highest prevalence of virulence genes (287/333) in which a high occurring variability was observed in the capsule biosynthesis and transport, O-linked flagellar glycosylation and lipooligosaccharide biosynthesis loci, with a great impact of phase-variation that led to 72 different virulence gene patterns among all isolates. High prevalence (> 90 %) of blaOXA-type β-lactamase genes and mutations in DNA gyrase gene associated with fluoroquinolones resistance were observed, and at a frequency similar to the tet(O) gene in C. jejuni (93 %) and C. coli (86 %), both of which also harboured resistance determinants to aminoglycosides with a higher occurrence rate in C. coli (43 %), that was the only species in which mutations in the 23S ribosomal subunit conferring resistance to erythromycin were identified (43 %). The present study constitutes the largest genomic survey of Campylobacter isolates in Spain providing insight into the prevalence and diversity of the pathogen along the poultry supply chain in the country.}},
  articleno    = {{110940}},
  author       = {{Ortega Sanz, Irene and Rovira, Jordi and Melero, Beatriz}},
  issn         = {{0168-1605}},
  journal      = {{INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY}},
  keywords     = {{Whole-genome sequencing,Genomics,Phylogeny,Virulence genes,Phase variation,Antimicrobial genes,MULTILOCUS SEQUENCE TYPES,CMEABC EFFLUX PUMP,ANTIBIOTIC-RESISTANCE,HUMAN-DISEASE,JEJUNI,COLI,POULTRY,PATTERNS,EMERGENCE,ALGORITHM}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{Whole-genome comparative analysis of the genetic, virulence and antimicrobial resistance diversity of Campylobacter spp. from Spain}},
  url          = {{http://doi.org/10.1016/j.ijfoodmicro.2024.110940}},
  volume       = {{427}},
  year         = {{2025}},
}

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