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Subpar reporting of pre-analytical variables in RNA-focused blood plasma studies

Céleste Van Der Schueren (UGent) , Philippe Decruyenaere (UGent) , Francisco Avila Cobos (UGent) , Johanna Bult, Jill Deleu (UGent) , Lydia Dipalo, Hetty Helsmoortel (UGent) , Eva Hulstaert, Annelien Morlion (UGent) , Elena Ramos Varas (UGent) , et al.
(2024) OncoPoint Symposium, 10th, Abstracts.
Author
Organization
Abstract
Extracellular RNA (cell-free RNA; exRNA) from blood-derived liquid biopsies is an appealing, minimally invasive source of disease biomarkers. As pre-analytical variables strongly influence exRNA measurements, their reporting is essential for meaningful interpretation and replication of results. The aim of this review was to chart to what extent pre-analytical variables are documented, to pinpoint shortcomings and to improve future reporting. In total, 200 blood plasma exRNA studies published in 2018 or 2023 were reviewed for annotation of 22 variables associated with blood collection, plasma preparation and RNA purification. Our results show that pre-analytical variables are poorly documented, with only three out of 22 variables described in over half of the publications. The percentage of variables reported ranged from 4.6% to 54.6% (mean 24.84%) in 2023 and from 4.6% to 57.1% (mean 28.60%) in 2018. Recommendations and guidelines (i.e., BRISQ, ASCO-CAP, BloodPAC, PPMPT, and CEN standards) have currently not resulted in improved reporting. In conclusion, our results highlight the lack of reporting pre-analytical variables in exRNA studies and advocate for a consistent use of available standards, endorsed by funders and journals.

Citation

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MLA
Van Der Schueren, Céleste, et al. “Subpar Reporting of Pre-Analytical Variables in RNA-Focused Blood Plasma Studies.” OncoPoint Symposium, 10th, Abstracts, 2024.
APA
Van Der Schueren, C., Decruyenaere, P., Avila Cobos, F., Bult, J., Deleu, J., Dipalo, L., … Decock, A. (2024). Subpar reporting of pre-analytical variables in RNA-focused blood plasma studies. OncoPoint Symposium, 10th, Abstracts. Presented at the 10th OncoPoint Symposium, Ghent, Belgium.
Chicago author-date
Van Der Schueren, Céleste, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Lydia Dipalo, Hetty Helsmoortel, et al. 2024. “Subpar Reporting of Pre-Analytical Variables in RNA-Focused Blood Plasma Studies.” In OncoPoint Symposium, 10th, Abstracts.
Chicago author-date (all authors)
Van Der Schueren, Céleste, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Lydia Dipalo, Hetty Helsmoortel, Eva Hulstaert, Annelien Morlion, Elena Ramos Varas, Kathleen Schoofs, Wim Trypsteen, Eveline Vanden Eynde, Hanne Van Droogenbroeck, Kimberly Verniers, Jo Vandesompele, and Anneleen Decock. 2024. “Subpar Reporting of Pre-Analytical Variables in RNA-Focused Blood Plasma Studies.” In OncoPoint Symposium, 10th, Abstracts.
Vancouver
1.
Van Der Schueren C, Decruyenaere P, Avila Cobos F, Bult J, Deleu J, Dipalo L, et al. Subpar reporting of pre-analytical variables in RNA-focused blood plasma studies. In: OncoPoint Symposium, 10th, Abstracts. 2024.
IEEE
[1]
C. Van Der Schueren et al., “Subpar reporting of pre-analytical variables in RNA-focused blood plasma studies,” in OncoPoint Symposium, 10th, Abstracts, Ghent, Belgium, 2024.
@inproceedings{01JC0VD4FEEAJSCGMQX58YKMQY,
  abstract     = {{Extracellular RNA (cell-free RNA; exRNA) from blood-derived liquid biopsies is an appealing, minimally invasive source of disease biomarkers. As pre-analytical variables strongly influence exRNA measurements, their reporting is essential for meaningful interpretation and replication of results. The aim of this review was to chart to what extent pre-analytical variables are documented, to pinpoint shortcomings and to improve future reporting. In total, 200 blood plasma exRNA studies published in 2018 or 2023 were reviewed for annotation of 22 variables associated with blood collection, plasma preparation and RNA purification. Our results show that pre-analytical variables are poorly documented, with only three out of 22 variables described in over half of the publications. The percentage of variables reported ranged from 4.6% to 54.6% (mean 24.84%) in 2023 and from 4.6% to 57.1% (mean 28.60%) in 2018. Recommendations and guidelines (i.e., BRISQ, ASCO-CAP, BloodPAC, PPMPT, and CEN standards) have currently not resulted in improved reporting. In conclusion, our results highlight the lack of reporting pre-analytical variables in exRNA studies and advocate for a consistent use of available standards, endorsed by funders and journals.}},
  author       = {{Van Der Schueren, Céleste and Decruyenaere, Philippe and Avila Cobos, Francisco and Bult, Johanna and Deleu, Jill and Dipalo, Lydia and Helsmoortel, Hetty and Hulstaert, Eva and Morlion, Annelien and Ramos Varas, Elena and Schoofs, Kathleen and Trypsteen, Wim and Vanden Eynde, Eveline and Van Droogenbroeck, Hanne and Verniers, Kimberly and Vandesompele, Jo and Decock, Anneleen}},
  booktitle    = {{OncoPoint Symposium, 10th, Abstracts}},
  language     = {{eng}},
  location     = {{Ghent, Belgium}},
  title        = {{Subpar reporting of pre-analytical variables in RNA-focused blood plasma studies}},
  url          = {{https://www.crig.ugent.be/en/events/oncopoint-symposium-2024}},
  year         = {{2024}},
}