
A nitrogen-specific interactome analysis sheds light on the role of the SnRK1 and TOR kinases in plant nitrogen signaling
- Author
- Freya Persyn (UGent) , Wouter Smagghe (UGent) , Dominique Eeckhout (UGent) , Toon Mertens (UGent) , Thomas Smorscek (UGent) , Nancy De Winne (UGent) , Geert Persiau (UGent) , Eveline Van De Slijke (UGent) , Nathalie Crepin (UGent) , Astrid Gadeyne (UGent) , Jelle Van Leene (UGent) and Geert De Jaeger (UGent)
- Organization
- Project
-
- Engineering Nitrogen Use Efficiency in plants based on the TOR/SnRK1 signalling pathway.
- Mapping and mining the TOR/SnRK1 interactome around nitrogen signaling
- A quest for high-yield and stress-resilient plants through combinatorial CRISPR screening based on TOR, SnRK1 and Trehalose-6-phosphate signalling networks.
- Dissecting TOR and SnRK1 signaling to engineer plant growth under limiting nitrogen supply
- Abstract
- Nitrogen (N) is of utmost importance for plant growth and development. Multiple studies have shown that N signaling is tightly coupled with carbon (C) levels, but the interplay between C/N metabolism and growth remains largely an enigma. Nonetheless, the protein kinases Sucrose Non-fermenting 1 (SNF1)-Related Kinase 1 (SnRK1) and Target Of Rapamycin (TOR), two ancient central metabolic regulators, are emerging as key integrators that link C/N status with growth. Despite their pivotal importance, the exact mechanisms behind the sensing of N status and its integration with C availability to drive metabolic decisions are largely unknown. Especially for SnRK1 it is not clear how this kinase responds to altered N levels. Therefore, we first monitored N-dependent SnRK1 kinase activity with an in vivo Separation of Phase-based Activity Reporter of Kinase (SPARK) sensor, revealing a contrasting N-dependency in Arabidopsis thaliana (Arabidopsis) shoot and root tissues. Next, using affinity purification (AP) and proximity labeling (PL) coupled to mass spectrometry (MS) experiments, we constructed a comprehensive SnRK1 and TOR interactome in Arabidopsis cell cultures during N-starved and N-repleted growth conditions. To broaden our understanding of the N-specificity of the TOR/SnRK1 signaling events, the resulting network was compared to corresponding C-related networks, identifying a large number of novel, N-specific interactors. Moreover, through integration of N-dependent transcriptome and phosphoproteome data, we were able to pinpoint additional N-dependent network components, highlighting for instance SnRK1 regulatory proteins that might function at the crosstalk of C/N signaling. Finally, confirmation of known and identification of novel SnRK1 interactors, such as Inositol-Requiring 1 (IRE1A) and the RAB GTPase RAB18, indicate that SnRK1, present at the ER, is involved in N signaling and autophagy induction.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01J91HAYGG917T6A583JZRDM19
- MLA
- Persyn, Freya, et al. “A Nitrogen-Specific Interactome Analysis Sheds Light on the Role of the SnRK1 and TOR Kinases in Plant Nitrogen Signaling.” MOLECULAR & CELLULAR PROTEOMICS, vol. 23, no. 10, 2024, doi:10.1016/j.mcpro.2024.100842.
- APA
- Persyn, F., Smagghe, W., Eeckhout, D., Mertens, T., Smorscek, T., De Winne, N., … De Jaeger, G. (2024). A nitrogen-specific interactome analysis sheds light on the role of the SnRK1 and TOR kinases in plant nitrogen signaling. MOLECULAR & CELLULAR PROTEOMICS, 23(10). https://doi.org/10.1016/j.mcpro.2024.100842
- Chicago author-date
- Persyn, Freya, Wouter Smagghe, Dominique Eeckhout, Toon Mertens, Thomas Smorscek, Nancy De Winne, Geert Persiau, et al. 2024. “A Nitrogen-Specific Interactome Analysis Sheds Light on the Role of the SnRK1 and TOR Kinases in Plant Nitrogen Signaling.” MOLECULAR & CELLULAR PROTEOMICS 23 (10). https://doi.org/10.1016/j.mcpro.2024.100842.
- Chicago author-date (all authors)
- Persyn, Freya, Wouter Smagghe, Dominique Eeckhout, Toon Mertens, Thomas Smorscek, Nancy De Winne, Geert Persiau, Eveline Van De Slijke, Nathalie Crepin, Astrid Gadeyne, Jelle Van Leene, and Geert De Jaeger. 2024. “A Nitrogen-Specific Interactome Analysis Sheds Light on the Role of the SnRK1 and TOR Kinases in Plant Nitrogen Signaling.” MOLECULAR & CELLULAR PROTEOMICS 23 (10). doi:10.1016/j.mcpro.2024.100842.
- Vancouver
- 1.Persyn F, Smagghe W, Eeckhout D, Mertens T, Smorscek T, De Winne N, et al. A nitrogen-specific interactome analysis sheds light on the role of the SnRK1 and TOR kinases in plant nitrogen signaling. MOLECULAR & CELLULAR PROTEOMICS. 2024;23(10).
- IEEE
- [1]F. Persyn et al., “A nitrogen-specific interactome analysis sheds light on the role of the SnRK1 and TOR kinases in plant nitrogen signaling,” MOLECULAR & CELLULAR PROTEOMICS, vol. 23, no. 10, 2024.
@article{01J91HAYGG917T6A583JZRDM19, abstract = {{Nitrogen (N) is of utmost importance for plant growth and development. Multiple studies have shown that N signaling is tightly coupled with carbon (C) levels, but the interplay between C/N metabolism and growth remains largely an enigma. Nonetheless, the protein kinases Sucrose Non-fermenting 1 (SNF1)-Related Kinase 1 (SnRK1) and Target Of Rapamycin (TOR), two ancient central metabolic regulators, are emerging as key integrators that link C/N status with growth. Despite their pivotal importance, the exact mechanisms behind the sensing of N status and its integration with C availability to drive metabolic decisions are largely unknown. Especially for SnRK1 it is not clear how this kinase responds to altered N levels. Therefore, we first monitored N-dependent SnRK1 kinase activity with an in vivo Separation of Phase-based Activity Reporter of Kinase (SPARK) sensor, revealing a contrasting N-dependency in Arabidopsis thaliana (Arabidopsis) shoot and root tissues. Next, using affinity purification (AP) and proximity labeling (PL) coupled to mass spectrometry (MS) experiments, we constructed a comprehensive SnRK1 and TOR interactome in Arabidopsis cell cultures during N-starved and N-repleted growth conditions. To broaden our understanding of the N-specificity of the TOR/SnRK1 signaling events, the resulting network was compared to corresponding C-related networks, identifying a large number of novel, N-specific interactors. Moreover, through integration of N-dependent transcriptome and phosphoproteome data, we were able to pinpoint additional N-dependent network components, highlighting for instance SnRK1 regulatory proteins that might function at the crosstalk of C/N signaling. Finally, confirmation of known and identification of novel SnRK1 interactors, such as Inositol-Requiring 1 (IRE1A) and the RAB GTPase RAB18, indicate that SnRK1, present at the ER, is involved in N signaling and autophagy induction.}}, articleno = {{100842}}, author = {{Persyn, Freya and Smagghe, Wouter and Eeckhout, Dominique and Mertens, Toon and Smorscek, Thomas and De Winne, Nancy and Persiau, Geert and Van De Slijke, Eveline and Crepin, Nathalie and Gadeyne, Astrid and Van Leene, Jelle and De Jaeger, Geert}}, issn = {{1535-9476}}, journal = {{MOLECULAR & CELLULAR PROTEOMICS}}, language = {{eng}}, number = {{10}}, pages = {{22}}, title = {{A nitrogen-specific interactome analysis sheds light on the role of the SnRK1 and TOR kinases in plant nitrogen signaling}}, url = {{http://doi.org/10.1016/j.mcpro.2024.100842}}, volume = {{23}}, year = {{2024}}, }
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