Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza
- Author
- Xiaole Cui (UGent) , Pieter Vervaeke (UGent) , Ya Gao (UGent) , Lisa Opsomer, Qing Sun (UGent) , Janne Snoeck (UGent) , Bert Devriendt (UGent) , Zifu Zhong (UGent) and Niek Sanders (UGent)
- Organization
- Project
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- Non-covalent hapten-immunogen next generation anti-opioid nanovaccines
- Design and pre-clincial evaluation of self-replicating mRNA therapeutics that produce protein drugs under control of small, non-toxic molecules
- A basic whole-body bioluminescent and fluorescent in vivo imaging system for small laboratory animals
- Cationic polymers with controlled charge separation for non-viral RNA delivery
- Abstract
- This study reports on the immunogenicity and biodistribution of H5 hemagglutinin (HA)-based self-amplifying (sa) mRNA vaccines in mice. Four sa-mRNA vaccines encoding either a secreted full-length HA, a secreted HA head domain, a secreted HA stalk domain, or a full-length membrane-anchored HA were investigated. All vaccines elicited an adaptive immune response. However, the full-length HA sa-RNA vaccines demonstrated superior performance compared to head and stalk domain vaccines. The antibody titers positively correlated with the vaccine dose. Cellular immune responses and antigen-specific IgA antibodies in the lungs were also observed. The comparison of the sa-mRNA vaccines encoding the secreted and membrane-anchored full-length HA revealed that anchoring of the HA to the membrane significantly enhanced the antibody and cellular responses. In addition to the injection site, the intramuscularly injected sa-mRNA-LNPs were also detected in the draining lymph nodes, spleen, and to a lesser extent, in the lung, kidney, liver, and heart.
- Keywords
- VIRUS HEMAGGLUTININ, T-LYMPHOCYTES, PROTECTS MICE, A VIRUS, ANTIBODIES, EFFICACY, CELLS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01J8Q4DW50X2ZY1M22WWSEVN23
- MLA
- Cui, Xiaole, et al. “Immunogenicity and Biodistribution of Lipid Nanoparticle Formulated Self-Amplifying MRNA Vaccines against H5 Avian Influenza.” NPJ VACCINES, vol. 9, no. 1, Nature Portfolio, 2024, doi:10.1038/s41541-024-00932-x.
- APA
- Cui, X., Vervaeke, P., Gao, Y., Opsomer, L., Sun, Q., Snoeck, J., … Sanders, N. (2024). Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza. NPJ VACCINES, 9(1). https://doi.org/10.1038/s41541-024-00932-x
- Chicago author-date
- Cui, Xiaole, Pieter Vervaeke, Ya Gao, Lisa Opsomer, Qing Sun, Janne Snoeck, Bert Devriendt, Zifu Zhong, and Niek Sanders. 2024. “Immunogenicity and Biodistribution of Lipid Nanoparticle Formulated Self-Amplifying MRNA Vaccines against H5 Avian Influenza.” NPJ VACCINES 9 (1). https://doi.org/10.1038/s41541-024-00932-x.
- Chicago author-date (all authors)
- Cui, Xiaole, Pieter Vervaeke, Ya Gao, Lisa Opsomer, Qing Sun, Janne Snoeck, Bert Devriendt, Zifu Zhong, and Niek Sanders. 2024. “Immunogenicity and Biodistribution of Lipid Nanoparticle Formulated Self-Amplifying MRNA Vaccines against H5 Avian Influenza.” NPJ VACCINES 9 (1). doi:10.1038/s41541-024-00932-x.
- Vancouver
- 1.Cui X, Vervaeke P, Gao Y, Opsomer L, Sun Q, Snoeck J, et al. Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza. NPJ VACCINES. 2024;9(1).
- IEEE
- [1]X. Cui et al., “Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza,” NPJ VACCINES, vol. 9, no. 1, 2024.
@article{01J8Q4DW50X2ZY1M22WWSEVN23,
abstract = {{This study reports on the immunogenicity and biodistribution of H5 hemagglutinin (HA)-based self-amplifying (sa) mRNA vaccines in mice. Four sa-mRNA vaccines encoding either a secreted full-length HA, a secreted HA head domain, a secreted HA stalk domain, or a full-length membrane-anchored HA were investigated. All vaccines elicited an adaptive immune response. However, the full-length HA sa-RNA vaccines demonstrated superior performance compared to head and stalk domain vaccines. The antibody titers positively correlated with the vaccine dose. Cellular immune responses and antigen-specific IgA antibodies in the lungs were also observed. The comparison of the sa-mRNA vaccines encoding the secreted and membrane-anchored full-length HA revealed that anchoring of the HA to the membrane significantly enhanced the antibody and cellular responses. In addition to the injection site, the intramuscularly injected sa-mRNA-LNPs were also detected in the draining lymph nodes, spleen, and to a lesser extent, in the lung, kidney, liver, and heart.}},
articleno = {{138}},
author = {{Cui, Xiaole and Vervaeke, Pieter and Gao, Ya and Opsomer, Lisa and Sun, Qing and Snoeck, Janne and Devriendt, Bert and Zhong, Zifu and Sanders, Niek}},
issn = {{2059-0105}},
journal = {{NPJ VACCINES}},
keywords = {{VIRUS HEMAGGLUTININ,T-LYMPHOCYTES,PROTECTS MICE,A VIRUS,ANTIBODIES,EFFICACY,CELLS}},
language = {{eng}},
number = {{1}},
pages = {{13}},
publisher = {{Nature Portfolio}},
title = {{Immunogenicity and biodistribution of lipid nanoparticle formulated self-amplifying mRNA vaccines against H5 avian influenza}},
url = {{http://doi.org/10.1038/s41541-024-00932-x}},
volume = {{9}},
year = {{2024}},
}
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