Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice
- Author
- Adillah Gul (UGent) , Lecia L. Pewe, Patrick Willems (UGent) , Rupert Mayer (UGent) , Fabien Henri Thery (UGent) , Caroline Asselman (UGent) , Ilke Aernout (UGent) , Rein Verbeke (UGent) , Denzel Eggermont (UGent) , Laura Van Moortel (UGent) , Ellen Upton, Yifeng Zhang, Katie Boucher (UGent) , Laia Miret Casals (UGent) , Hans Demol (UGent) , Stefaan De Smedt (UGent) , Ine Lentacker (UGent) , Lilliana Radoshevich, John T. Harty and Francis Impens (UGent)
- Organization
- Project
-
- Proteomics-based characterization of the protein interplay between pathogenic bacteria and their host.
- Characterizing ISG15 conjugation as a molecular signal for protein oligomerization and translocation in host immunity
- Systemic redoxome profiling in plants: catching the ROS wave
- Understanding and modulating the pro-inflammatory activity of lipid nanoparticles (LNP) in the COVID-19 mRNA vaccines: towards the development of more effective LNP formulations.
- Exploration of an mRNA based nanovaccine platform and immunopeptidomics for the development of more effective tuberculosis vaccines.
- Exploring nanobodies against Interferon Stimulated Gene 15 (ISG15) to combat autoimmune and infectious diseases.
- Proteomics-derived epitopes for dramatically improved anticancer and antibacterial vaccine development
- Immunopeptidomics-based Development of Next-Generation Bacterial mRNA Vaccines
- Abstract
- Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8+ T cell response, but very few T cell epitopes are known in mice as a common animal infection model for listeriosis. To identify epitopes we screened for Listeria immunopeptides presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics. We mapped more than 6,000 mouse self-peptides presented on MHC Class I molecules, including 12 high confident Listeria peptides from 12 different bacterial proteins. Bacterial immunopeptides with confirmed fragmentation spectra were further tested for their potential to activate CD8+ T cells, revealing VTYNYINI from the putative cell wall surface anchor family protein LMON_0576 as a novel bona fide peptide epitope. The epitope showed high biological potency in a prime boost model and can be used as a research tool to probe CD8+ T cell responses in mouse models of Listeria infection. Together, our results demonstrate the power of immunopeptidomics for bacterial antigen identification.
- Keywords
- Listeria monocytogenes, MHC, bacterial infection, epitope discovery, mass spectrometry, MASS-SPECTROMETRY, ANTIGEN PRESENTATION, SURFACE PROTEIN, T-CELLS, BACTERIAL, VACCINE, IDENTIFICATION, MOLECULES, RESPONSES, MEMORY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01J6W2NH91DA5HHNWAWQHADZ3T
- MLA
- Gul, Adillah, et al. “Immunopeptidomics Mapping of Listeria Monocytogenes T Cell Epitopes in Mice.” MOLECULAR & CELLULAR PROTEOMICS, vol. 23, no. 9, 2024, doi:10.1016/j.mcpro.2024.100829.
- APA
- Gul, A., Pewe, L. L., Willems, P., Mayer, R., Thery, F. H., Asselman, C., … Impens, F. (2024). Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice. MOLECULAR & CELLULAR PROTEOMICS, 23(9). https://doi.org/10.1016/j.mcpro.2024.100829
- Chicago author-date
- Gul, Adillah, Lecia L. Pewe, Patrick Willems, Rupert Mayer, Fabien Henri Thery, Caroline Asselman, Ilke Aernout, et al. 2024. “Immunopeptidomics Mapping of Listeria Monocytogenes T Cell Epitopes in Mice.” MOLECULAR & CELLULAR PROTEOMICS 23 (9). https://doi.org/10.1016/j.mcpro.2024.100829.
- Chicago author-date (all authors)
- Gul, Adillah, Lecia L. Pewe, Patrick Willems, Rupert Mayer, Fabien Henri Thery, Caroline Asselman, Ilke Aernout, Rein Verbeke, Denzel Eggermont, Laura Van Moortel, Ellen Upton, Yifeng Zhang, Katie Boucher, Laia Miret Casals, Hans Demol, Stefaan De Smedt, Ine Lentacker, Lilliana Radoshevich, John T. Harty, and Francis Impens. 2024. “Immunopeptidomics Mapping of Listeria Monocytogenes T Cell Epitopes in Mice.” MOLECULAR & CELLULAR PROTEOMICS 23 (9). doi:10.1016/j.mcpro.2024.100829.
- Vancouver
- 1.Gul A, Pewe LL, Willems P, Mayer R, Thery FH, Asselman C, et al. Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice. MOLECULAR & CELLULAR PROTEOMICS. 2024;23(9).
- IEEE
- [1]A. Gul et al., “Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice,” MOLECULAR & CELLULAR PROTEOMICS, vol. 23, no. 9, 2024.
@article{01J6W2NH91DA5HHNWAWQHADZ3T,
abstract = {{Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8+ T cell response, but very few T cell epitopes are known in mice as a common animal infection model for listeriosis. To identify epitopes we screened for Listeria immunopeptides presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics. We mapped more than 6,000 mouse self-peptides presented on MHC Class I molecules, including 12 high confident Listeria peptides from 12 different bacterial proteins. Bacterial immunopeptides with confirmed fragmentation spectra were further tested for their potential to activate CD8+ T cells, revealing VTYNYINI from the putative cell wall surface anchor family protein LMON_0576 as a novel bona fide peptide epitope. The epitope showed high biological potency in a prime boost model and can be used as a research tool to probe CD8+ T cell responses in mouse models of Listeria infection. Together, our results demonstrate the power of immunopeptidomics for bacterial antigen identification.}},
articleno = {{100829}},
author = {{Gul, Adillah and Pewe, Lecia L. and Willems, Patrick and Mayer, Rupert and Thery, Fabien Henri and Asselman, Caroline and Aernout, Ilke and Verbeke, Rein and Eggermont, Denzel and Van Moortel, Laura and Upton, Ellen and Zhang, Yifeng and Boucher, Katie and Miret Casals, Laia and Demol, Hans and De Smedt, Stefaan and Lentacker, Ine and Radoshevich, Lilliana and Harty, John T. and Impens, Francis}},
issn = {{1535-9476}},
journal = {{MOLECULAR & CELLULAR PROTEOMICS}},
keywords = {{Listeria monocytogenes,MHC,bacterial infection,epitope discovery,mass spectrometry,MASS-SPECTROMETRY,ANTIGEN PRESENTATION,SURFACE PROTEIN,T-CELLS,BACTERIAL,VACCINE,IDENTIFICATION,MOLECULES,RESPONSES,MEMORY}},
language = {{eng}},
number = {{9}},
pages = {{17}},
title = {{Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice}},
url = {{http://doi.org/10.1016/j.mcpro.2024.100829}},
volume = {{23}},
year = {{2024}},
}
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