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Dose-dependent responses : a preliminary investigation into the olfactory effects of essential oil concentrations on canine behavior

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Abstract
The positive impact of essential oils (EOs) on stress release has been demonstrated in both humans and dogs. Among the EOs known for their anxiety-reducing properties, including Cananga odorata, Citrus aurantium, Cupressus sempervirens, Lavandula angustifolia, and Litsea citrata, there is a lack of consensus on the optimal concentration for efficacy. This exploratory study sought to investigate the effects of olfactory enrichment with a blend of these EOs on dogs introduced to an unfamiliar environment. The authors sought to determine the minimum concentration required to achieve increased relaxation. In a randomized controlled crossover study design, 54 dogs were exposed to 0, 1, 5, and 10 drops of the EO blend applied to their collars before entering an unfamiliar room with their owners. Behavioral observations were employed to quantify the total duration of activity and relaxation related behaviours for each dog under each treatment condition. A significant difference in panting was identified among the treatments (chi 2(3) = 9.88; p = 0.020). Dunn-Bonferroni post-hoc tests revealed a significant reduction in panting during the 10 drops treatment compared to the control treatment (p = 0.047). No significant differences were observed for other behaviors. To provide a comprehensive overview of behavioral tendencies in this canine population, owners also completed the Canine Behavioral Assessment and Research Questionnaire (C-BARQ), revealing low scores for anxiety in the study group. These preliminary findings suggest that a concentration of 10 drops of the EO blend on a dog's collar induces increased relaxation, specifically reflected in decreased panting behavior. Lower concentrations did not exhibit a significant relationship with the observed behaviors. These initial findings underscore the importance of exposing dogs to an appropriate concentration of EOs when exploring their potential benefits on welfare among dogs with low anxiety levels. Further research in this area is crucial for a comprehensive understanding of the potential benefits of EOs for canine welfare.
Keywords
Dog, Relaxation, Olfactory enrichment, Essential oils, Behavior, CITRUS-AURANTIUM L., DOGS, ANXIETY, STRESS, AROMATHERAPY, STIMULATION, PERCEPTION, INHALATION, FAMILIARIS, CORTISOL

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MLA
Haverbeke, Anouck, et al. “Dose-Dependent Responses : A Preliminary Investigation into the Olfactory Effects of Essential Oil Concentrations on Canine Behavior.” VETERINARY RESEARCH COMMUNICATIONS, vol. 48, no. 5, 2024, pp. 3387–96, doi:10.1007/s11259-024-10466-1.
APA
Haverbeke, A., Uccheddu, S., Reinert, C., Tertemiz, S., Arnouts, H., & Sannen, A. (2024). Dose-dependent responses : a preliminary investigation into the olfactory effects of essential oil concentrations on canine behavior. VETERINARY RESEARCH COMMUNICATIONS, 48(5), 3387–3396. https://doi.org/10.1007/s11259-024-10466-1
Chicago author-date
Haverbeke, Anouck, Stefania Uccheddu, Clemens Reinert, Selene Tertemiz, Heidi Arnouts, and Adinda Sannen. 2024. “Dose-Dependent Responses : A Preliminary Investigation into the Olfactory Effects of Essential Oil Concentrations on Canine Behavior.” VETERINARY RESEARCH COMMUNICATIONS 48 (5): 3387–96. https://doi.org/10.1007/s11259-024-10466-1.
Chicago author-date (all authors)
Haverbeke, Anouck, Stefania Uccheddu, Clemens Reinert, Selene Tertemiz, Heidi Arnouts, and Adinda Sannen. 2024. “Dose-Dependent Responses : A Preliminary Investigation into the Olfactory Effects of Essential Oil Concentrations on Canine Behavior.” VETERINARY RESEARCH COMMUNICATIONS 48 (5): 3387–3396. doi:10.1007/s11259-024-10466-1.
Vancouver
1.
Haverbeke A, Uccheddu S, Reinert C, Tertemiz S, Arnouts H, Sannen A. Dose-dependent responses : a preliminary investigation into the olfactory effects of essential oil concentrations on canine behavior. VETERINARY RESEARCH COMMUNICATIONS. 2024;48(5):3387–96.
IEEE
[1]
A. Haverbeke, S. Uccheddu, C. Reinert, S. Tertemiz, H. Arnouts, and A. Sannen, “Dose-dependent responses : a preliminary investigation into the olfactory effects of essential oil concentrations on canine behavior,” VETERINARY RESEARCH COMMUNICATIONS, vol. 48, no. 5, pp. 3387–3396, 2024.
@article{01J4S6P8V6XX6P5D06NHNJ2PYJ,
  abstract     = {{The positive impact of essential oils (EOs) on stress release has been demonstrated in both humans and dogs. Among the EOs known for their anxiety-reducing properties, including Cananga odorata, Citrus aurantium, Cupressus sempervirens, Lavandula angustifolia, and Litsea citrata, there is a lack of consensus on the optimal concentration for efficacy. This exploratory study sought to investigate the effects of olfactory enrichment with a blend of these EOs on dogs introduced to an unfamiliar environment. The authors sought to determine the minimum concentration required to achieve increased relaxation. In a randomized controlled crossover study design, 54 dogs were exposed to 0, 1, 5, and 10 drops of the EO blend applied to their collars before entering an unfamiliar room with their owners. Behavioral observations were employed to quantify the total duration of activity and relaxation related behaviours for each dog under each treatment condition. A significant difference in panting was identified among the treatments (chi 2(3) = 9.88; p = 0.020). Dunn-Bonferroni post-hoc tests revealed a significant reduction in panting during the 10 drops treatment compared to the control treatment (p = 0.047). No significant differences were observed for other behaviors. To provide a comprehensive overview of behavioral tendencies in this canine population, owners also completed the Canine Behavioral Assessment and Research Questionnaire (C-BARQ), revealing low scores for anxiety in the study group. These preliminary findings suggest that a concentration of 10 drops of the EO blend on a dog's collar induces increased relaxation, specifically reflected in decreased panting behavior. Lower concentrations did not exhibit a significant relationship with the observed behaviors. These initial findings underscore the importance of exposing dogs to an appropriate concentration of EOs when exploring their potential benefits on welfare among dogs with low anxiety levels. Further research in this area is crucial for a comprehensive understanding of the potential benefits of EOs for canine welfare.}},
  author       = {{Haverbeke, Anouck and Uccheddu, Stefania and Reinert, Clemens and Tertemiz, Selene and Arnouts, Heidi and Sannen, Adinda}},
  issn         = {{0165-7380}},
  journal      = {{VETERINARY RESEARCH COMMUNICATIONS}},
  keywords     = {{Dog,Relaxation,Olfactory enrichment,Essential oils,Behavior,CITRUS-AURANTIUM L.,DOGS,ANXIETY,STRESS,AROMATHERAPY,STIMULATION,PERCEPTION,INHALATION,FAMILIARIS,CORTISOL}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{3387--3396}},
  title        = {{Dose-dependent responses : a preliminary investigation into the olfactory effects of essential oil concentrations on canine behavior}},
  url          = {{http://doi.org/10.1007/s11259-024-10466-1}},
  volume       = {{48}},
  year         = {{2024}},
}

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