@article{01J4KFJN4JVE62DRW4EWJ6F9XG,
  abstract     = {{This study aimed to evaluate an expanded matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS) database for the identification of Haemophilus species other than H. influenzae (Hi). A total of 144 Haemophilus species, cultured from respiratory samples from people (living) with cystic fibrosis, were identified with MALDI-TOF MS and 16S rRNA sequencing. Of these, 99 Haemophilus strains showed >99% similarity with the best matching strain in the National Center for Biotechnology Information (NCBI) database and were assigned to a single Haemophilus subspecies using both MALDI-TOF MS and 16S rRNA sequencing. The MS profiles of a subset of strains (n = 58/99) were added to the Bruker MALDI-TOF MS database. Subsequently, 270 different strains that were analyzed previously in a routine setting were re-analyzed. 16S rRNA sequencing reliably identified 99/144 Haemophilus strains (>99% similarity). H. haemolyticus 16S rRNA identification was suboptimal since only 3/21 H. haemolyticus strains attained a similarity of >99% with H. haemolyticus 16S rRNA sequence in the NCBI database. Expansion of the MALDI-TOF MS database improved the number of reliable identifications only moderately for H. haemolyticus, H. influenzae and H. paraphrohaemolyticus (<10%). By contrast, improved identification was more outspoken for H.parahaemolyticus, H. parainfluenzae, H. sputorum and H. pittmaniae (>85%). 16S rRNA sequencing is a valuable method for the identification of Haemophilus sp. other than Hi. Expansion of the MALDI-TOF MS database, based on 16S rRNA sequencing results, increased the proportion of reliable identifications and in this study resulted in an increase of 10% of Haemophilus sp. other than Hi strain identifications.}},
  author       = {{Willems, Eva and Hamerlinck, Hannelore and Messiaen, Anne-Sophie and Schelstraete, Petra and Van Braeckel, Eva and Vande Weygaerde, Yannick and Verhasselt, Bruno and Boelens, Jerina and Vandendriessche, Stien}},
  issn         = {{1784-3286}},
  journal      = {{ACTA CLINICA BELGICA}},
  keywords     = {{16S rRNA sequencing,MALDI-TOF MS,Haemophilus,cystic fibrosis,bacterial identification}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{243--249}},
  title        = {{Expansion of MALDI-TOF MS database as a strategy for identification of Haemophilus species other than H. influenzae}},
  url          = {{http://doi.org/10.1080/17843286.2024.2386216}},
  volume       = {{79}},
  year         = {{2024}},
}

Altmetric

View in Altmetric

Web of Science

Times cited: