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Actinobacteria as promising biocontrol agents for in vitro and in planta degradation and detoxification of Zearalenone

Larissa De Troyer (UGent) , Noémie De Zutter (UGent) , Sarah De Saeger (UGent) , Frédéric Dumoulin (UGent) , Siska Croubels (UGent) , Siegrid De Baere (UGent) , Leen De Gelder (UGent) and Kris Audenaert (UGent)
(2024) TOXINS. 16(6).
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Abstract
Zearalenone (ZEN) is a prevalent mycotoxin found in grains and grain-derived products, inducing adverse health effects in both animals and humans. The in-field application of microorganisms to degrade and detoxify ZEN is a promising strategy to enhance the safety of food and feed. In this study, we investigated the potential of three actinobacterial strains to degrade and detoxify ZEN in vitro and in planta on wheat ears. The residual ZEN concentration and toxicity in the samples were analysed with UHPLC-MS/MS and a bioluminescence BLYES assay, respectively. Streptomyces rimosus subsp. rimosus LMG19352 could completely degrade and detoxify 5 mg/L ZEN in LB broth within 24 h, along with significant reductions in ZEN concentration both in a minimal medium (MM) and on wheat ears. Additionally, it was the only strain that showed a significant colonisation of these ears. Rhodococcus sp. R25614 exhibited partial but significant degradation in LB broth and MM, whereas Streptomyces sp. LMG16995 degraded and detoxified ZEN in LB broth after 72 h by 39% and 33%, respectively. Although all three actinobacterial strains demonstrated the metabolic capability to degrade and detoxify ZEN in vitro, only S. rimosus subsp. rimosus LMG19352 showed promising potential to mitigate ZEN in planta. This distinction underscores the importance of incorporating in planta screening assays for assessing the potential of mycotoxin-biotransforming microorganisms as biocontrol agents.
Keywords
zearalenone, actinobacteria, biotransformation, degradation, detoxification, wheat, biocontrol, PHASE-I, FUSARIUM, BIOTRANSFORMATION, DEOXYNIVALENOL, MYCOTOXINS, PARAMETERS, REDUCTION, CELLS

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MLA
De Troyer, Larissa, et al. “Actinobacteria as Promising Biocontrol Agents for in Vitro and in Planta Degradation and Detoxification of Zearalenone.” TOXINS, vol. 16, no. 6, 2024, doi:10.3390/toxins16060253.
APA
De Troyer, L., De Zutter, N., De Saeger, S., Dumoulin, F., Croubels, S., De Baere, S., … Audenaert, K. (2024). Actinobacteria as promising biocontrol agents for in vitro and in planta degradation and detoxification of Zearalenone. TOXINS, 16(6). https://doi.org/10.3390/toxins16060253
Chicago author-date
De Troyer, Larissa, Noémie De Zutter, Sarah De Saeger, Frédéric Dumoulin, Siska Croubels, Siegrid De Baere, Leen De Gelder, and Kris Audenaert. 2024. “Actinobacteria as Promising Biocontrol Agents for in Vitro and in Planta Degradation and Detoxification of Zearalenone.” TOXINS 16 (6). https://doi.org/10.3390/toxins16060253.
Chicago author-date (all authors)
De Troyer, Larissa, Noémie De Zutter, Sarah De Saeger, Frédéric Dumoulin, Siska Croubels, Siegrid De Baere, Leen De Gelder, and Kris Audenaert. 2024. “Actinobacteria as Promising Biocontrol Agents for in Vitro and in Planta Degradation and Detoxification of Zearalenone.” TOXINS 16 (6). doi:10.3390/toxins16060253.
Vancouver
1.
De Troyer L, De Zutter N, De Saeger S, Dumoulin F, Croubels S, De Baere S, et al. Actinobacteria as promising biocontrol agents for in vitro and in planta degradation and detoxification of Zearalenone. TOXINS. 2024;16(6).
IEEE
[1]
L. De Troyer et al., “Actinobacteria as promising biocontrol agents for in vitro and in planta degradation and detoxification of Zearalenone,” TOXINS, vol. 16, no. 6, 2024.
@article{01J1FC4Z8HA7DRPRXJ7176HRTY,
  abstract     = {{Zearalenone (ZEN) is a prevalent mycotoxin found in grains and grain-derived products, inducing adverse health effects in both animals and humans. The in-field application of microorganisms to degrade and detoxify ZEN is a promising strategy to enhance the safety of food and feed. In this study, we investigated the potential of three actinobacterial strains to degrade and detoxify ZEN in vitro and in planta on wheat ears. The residual ZEN concentration and toxicity in the samples were analysed with UHPLC-MS/MS and a bioluminescence BLYES assay, respectively. Streptomyces rimosus subsp. rimosus LMG19352 could completely degrade and detoxify 5 mg/L ZEN in LB broth within 24 h, along with significant reductions in ZEN concentration both in a minimal medium (MM) and on wheat ears. Additionally, it was the only strain that showed a significant colonisation of these ears. Rhodococcus sp. R25614 exhibited partial but significant degradation in LB broth and MM, whereas Streptomyces sp. LMG16995 degraded and detoxified ZEN in LB broth after 72 h by 39% and 33%, respectively. Although all three actinobacterial strains demonstrated the metabolic capability to degrade and detoxify ZEN in vitro, only S. rimosus subsp. rimosus LMG19352 showed promising potential to mitigate ZEN in planta. This distinction underscores the importance of incorporating in planta screening assays for assessing the potential of mycotoxin-biotransforming microorganisms as biocontrol agents.}},
  articleno    = {{253}},
  author       = {{De Troyer, Larissa and De Zutter, Noémie and De Saeger, Sarah and Dumoulin, Frédéric and Croubels, Siska and De Baere, Siegrid and De Gelder, Leen and Audenaert, Kris}},
  issn         = {{2072-6651}},
  journal      = {{TOXINS}},
  keywords     = {{zearalenone,actinobacteria,biotransformation,degradation,detoxification,wheat,biocontrol,PHASE-I,FUSARIUM,BIOTRANSFORMATION,DEOXYNIVALENOL,MYCOTOXINS,PARAMETERS,REDUCTION,CELLS}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{20}},
  title        = {{Actinobacteria as promising biocontrol agents for in vitro and in planta degradation and detoxification of Zearalenone}},
  url          = {{http://doi.org/10.3390/toxins16060253}},
  volume       = {{16}},
  year         = {{2024}},
}
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