High-throughput nanoflow proteomics using a dual-column electrospray source
- Author
- An Staes (UGent) , Katie Boucher (UGent) , Sara Dufour (UGent) , Teresa Maia (UGent) , Evy Timmerman (UGent) , Delphi Van Haver (UGent) , Jarne Pauwels (UGent) , Hans Demol (UGent) , Jonathan Vandenbussche (UGent) , Kris Gevaert (UGent) , Francis Impens (UGent) and Simon Devos (UGent)
- Organization
- Abstract
- With current trends in proteomics, especially regarding clinical and low input (to single cell) samples, it is increasingly important to both maximize the throughput of the analysis and maintain as much sensitivity as possible. The new generation of mass spectrometers (MS) are taking a huge leap in sensitivity, allowing analysis of samples with shorter liquid chromatography (LC) methods while digging as deep in the proteome. However, the throughput can be doubled by implementing a dual column nano-LC-MS configuration. For this purpose, we used a dual-column setup with a two-outlet electrospray source and compared it to a classic dual-column setup with a single-outlet source.
- Keywords
- QUANTITATIVE PROTEOMICS, SYSTEM, CARRYOVER, COMET
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01J1CDPVHGH5RNBBMC9Y0K9N7P
- MLA
- Staes, An, et al. “High-Throughput Nanoflow Proteomics Using a Dual-Column Electrospray Source.” ANALYTICAL CHEMISTRY, vol. 96, no. 17, 2024, pp. 6534–39, doi:10.1021/acs.analchem.4c00845.
- APA
- Staes, A., Boucher, K., Dufour, S., Maia, T., Timmerman, E., Van Haver, D., … Devos, S. (2024). High-throughput nanoflow proteomics using a dual-column electrospray source. ANALYTICAL CHEMISTRY, 96(17), 6534–6539. https://doi.org/10.1021/acs.analchem.4c00845
- Chicago author-date
- Staes, An, Katie Boucher, Sara Dufour, Teresa Maia, Evy Timmerman, Delphi Van Haver, Jarne Pauwels, et al. 2024. “High-Throughput Nanoflow Proteomics Using a Dual-Column Electrospray Source.” ANALYTICAL CHEMISTRY 96 (17): 6534–39. https://doi.org/10.1021/acs.analchem.4c00845.
- Chicago author-date (all authors)
- Staes, An, Katie Boucher, Sara Dufour, Teresa Maia, Evy Timmerman, Delphi Van Haver, Jarne Pauwels, Hans Demol, Jonathan Vandenbussche, Kris Gevaert, Francis Impens, and Simon Devos. 2024. “High-Throughput Nanoflow Proteomics Using a Dual-Column Electrospray Source.” ANALYTICAL CHEMISTRY 96 (17): 6534–6539. doi:10.1021/acs.analchem.4c00845.
- Vancouver
- 1.Staes A, Boucher K, Dufour S, Maia T, Timmerman E, Van Haver D, et al. High-throughput nanoflow proteomics using a dual-column electrospray source. ANALYTICAL CHEMISTRY. 2024;96(17):6534–9.
- IEEE
- [1]A. Staes et al., “High-throughput nanoflow proteomics using a dual-column electrospray source,” ANALYTICAL CHEMISTRY, vol. 96, no. 17, pp. 6534–6539, 2024.
@article{01J1CDPVHGH5RNBBMC9Y0K9N7P,
abstract = {{With current trends in proteomics, especially regarding clinical and low input (to single cell) samples, it is increasingly important to both maximize the throughput of the analysis and maintain as much sensitivity as possible. The new generation of mass spectrometers (MS) are taking a huge leap in sensitivity, allowing analysis of samples with shorter liquid chromatography (LC) methods while digging as deep in the proteome. However, the throughput can be doubled by implementing a dual column nano-LC-MS configuration. For this purpose, we used a dual-column setup with a two-outlet electrospray source and compared it to a classic dual-column setup with a single-outlet source.}},
author = {{Staes, An and Boucher, Katie and Dufour, Sara and Maia, Teresa and Timmerman, Evy and Van Haver, Delphi and Pauwels, Jarne and Demol, Hans and Vandenbussche, Jonathan and Gevaert, Kris and Impens, Francis and Devos, Simon}},
issn = {{0003-2700}},
journal = {{ANALYTICAL CHEMISTRY}},
keywords = {{QUANTITATIVE PROTEOMICS,SYSTEM,CARRYOVER,COMET}},
language = {{eng}},
number = {{17}},
pages = {{6534--6539}},
title = {{High-throughput nanoflow proteomics using a dual-column electrospray source}},
url = {{http://doi.org/10.1021/acs.analchem.4c00845}},
volume = {{96}},
year = {{2024}},
}
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