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Characterization of SARS-CoV-2 spike mutations important for infection of mice and escape from human immune sera

Raveen Rathnasinghe, Sonia Jangra, Chengjin Ye, Anastasija Cupic, Gagandeep Singh, Carles Martínez-Romero, Lubbertus C. F. Mulder, Thomas Kehrer, Soner Yildiz, Angela Choi, et al.
(2022) NATURE COMMUNICATIONS. 13(1).
Author
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Abstract
Due to differences in human and murine angiotensin converting enzyme 2 (ACE-2) receptor, initially available SARS-CoV-2 isolates could not infect mice. Here we show that serial passaging of USA-WA1/2020 strain in mouse lungs results in "mouse-adapted" SARS-CoV-2 (MA-SARS-CoV-2) with mutations in S, M, and N genes, and a twelve-nucleotide insertion in the S gene. MA-SARS-CoV-2 infection causes mild disease, with more pronounced morbidity depending on genetic background and in aged and obese mice. Two mutations in the S gene associated with mouse adaptation (N501Y, H655Y) are present in SARS-CoV-2 variants of concern (VoCs). N501Y in the receptor binding domain of viruses of the B.1.1.7, B.1.351, P.1 and B.1.1.529 lineages (Alpha, Beta, Gamma and Omicron variants) is associated with high transmissibility and allows VoCs to infect wild type mice. We further show that S protein mutations of MA-SARS-CoV-2 do not affect neutralization efficiency by human convalescent and post vaccination sera.
Keywords
VIRUS

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MLA
Rathnasinghe, Raveen, et al. “Characterization of SARS-CoV-2 Spike Mutations Important for Infection of Mice and Escape from Human Immune Sera.” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022, doi:10.1038/s41467-022-30763-0.
APA
Rathnasinghe, R., Jangra, S., Ye, C., Cupic, A., Singh, G., Martínez-Romero, C., … Schotsaert, M. (2022). Characterization of SARS-CoV-2 spike mutations important for infection of mice and escape from human immune sera. NATURE COMMUNICATIONS, 13(1). https://doi.org/10.1038/s41467-022-30763-0
Chicago author-date
Rathnasinghe, Raveen, Sonia Jangra, Chengjin Ye, Anastasija Cupic, Gagandeep Singh, Carles Martínez-Romero, Lubbertus C. F. Mulder, et al. 2022. “Characterization of SARS-CoV-2 Spike Mutations Important for Infection of Mice and Escape from Human Immune Sera.” NATURE COMMUNICATIONS 13 (1). https://doi.org/10.1038/s41467-022-30763-0.
Chicago author-date (all authors)
Rathnasinghe, Raveen, Sonia Jangra, Chengjin Ye, Anastasija Cupic, Gagandeep Singh, Carles Martínez-Romero, Lubbertus C. F. Mulder, Thomas Kehrer, Soner Yildiz, Angela Choi, Stephen T. Yeung, Ignacio Mena, Virginia Gillespie, Jana De Vrieze, Sadaf Aslam, Daniel Stadlbauer, David A. Meekins, Chester D. McDowell, Velmurugan Balaraman, Michael J. Corley, Juergen A. Richt, Bruno De Geest, Lisa Miorin, Giulio Kleiner, Miti Saksena, Komal Srivastava, Charles R. Gleason, Maria C. Bermúdez-González, Katherine F. Beach, Kayla T. Russo, Levy A. Sominsky, Emily D. Ferreri, Rachel L. Chernet, Lily Q. Eaker, Ashley-Beathrese T. Salimbangon, Denise Jurczyszak, Hala Alshammary, Wanni A. Mendez, Angela A. Amoako, Shelcie Fabre, Mahmoud H. Awawda, Amber S. Shin, [missing] PVI study group, Florian Krammer, Luis Martinez-Sobrido, Viviana Simon, Adolfo García-Sastre, and Michael Schotsaert. 2022. “Characterization of SARS-CoV-2 Spike Mutations Important for Infection of Mice and Escape from Human Immune Sera.” NATURE COMMUNICATIONS 13 (1). doi:10.1038/s41467-022-30763-0.
Vancouver
1.
Rathnasinghe R, Jangra S, Ye C, Cupic A, Singh G, Martínez-Romero C, et al. Characterization of SARS-CoV-2 spike mutations important for infection of mice and escape from human immune sera. NATURE COMMUNICATIONS. 2022;13(1).
IEEE
[1]
R. Rathnasinghe et al., “Characterization of SARS-CoV-2 spike mutations important for infection of mice and escape from human immune sera,” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022.
@article{01J0NKYNGGZ4JW16Y1HF40ZYA6,
  abstract     = {{Due to differences in human and murine angiotensin converting enzyme 2 (ACE-2) receptor, initially available SARS-CoV-2 isolates could not infect mice. Here we show that serial passaging of USA-WA1/2020 strain in mouse lungs results in "mouse-adapted" SARS-CoV-2 (MA-SARS-CoV-2) with mutations in S, M, and N genes, and a twelve-nucleotide insertion in the S gene. MA-SARS-CoV-2 infection causes mild disease, with more pronounced morbidity depending on genetic background and in aged and obese mice. Two mutations in the S gene associated with mouse adaptation (N501Y, H655Y) are present in SARS-CoV-2 variants of concern (VoCs). N501Y in the receptor binding domain of viruses of the B.1.1.7, B.1.351, P.1 and B.1.1.529 lineages (Alpha, Beta, Gamma and Omicron variants) is associated with high transmissibility and allows VoCs to infect wild type mice. We further show that S protein mutations of MA-SARS-CoV-2 do not affect neutralization efficiency by human convalescent and post vaccination sera.}},
  articleno    = {{3921}},
  author       = {{Rathnasinghe, Raveen and Jangra, Sonia and Ye, Chengjin and Cupic, Anastasija and Singh, Gagandeep and Martínez-Romero, Carles and Mulder, Lubbertus C. F. and Kehrer, Thomas and Yildiz, Soner and Choi, Angela and Yeung, Stephen T. and Mena, Ignacio and Gillespie, Virginia and De Vrieze, Jana and Aslam, Sadaf and Stadlbauer, Daniel and Meekins, David A. and McDowell, Chester D. and Balaraman, Velmurugan and Corley, Michael J. and Richt, Juergen A. and De Geest, Bruno and Miorin, Lisa and Kleiner, Giulio and Saksena, Miti and Srivastava, Komal and Gleason, Charles R. and Bermúdez-González, Maria C. and Beach, Katherine F. and Russo, Kayla T. and Sominsky, Levy A. and Ferreri, Emily D. and Chernet, Rachel L. and Eaker, Lily Q. and Salimbangon, Ashley-Beathrese T. and Jurczyszak, Denise and Alshammary, Hala and Mendez, Wanni A. and Amoako, Angela A. and Fabre, Shelcie and Awawda, Mahmoud H. and Shin, Amber S. and PVI study group, [missing] and Krammer, Florian and Martinez-Sobrido, Luis and Simon, Viviana and García-Sastre, Adolfo and Schotsaert, Michael}},
  issn         = {{2041-1723}},
  journal      = {{NATURE COMMUNICATIONS}},
  keywords     = {{VIRUS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{14}},
  title        = {{Characterization of SARS-CoV-2 spike mutations important for infection of mice and escape from human immune sera}},
  url          = {{http://doi.org/10.1038/s41467-022-30763-0}},
  volume       = {{13}},
  year         = {{2022}},
}
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