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Gold standard assessment of immunogenic cell death induced by photodynamic therapy : from in vitro to tumor mouse models and anti-cancer vaccination strategies

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Abstract
The discovery of the concept of immunogenic cell death (ICD) is a cornerstone in the development of novel anti-cancer immunotherapeutic approaches. Induction of the ICD pathway by specific anti-cancer therapeutic regimens can eliminate cancer cells by directly killing them during therapy and by activation of strong and specific anti-cancer immunity, leading to a long-lasting immunological memory that prevents cancer recurrence. ICD encompasses different forms of regulated cell death and can be triggered by many anti-cancer treatment modalities, including photodynamic therapy (PDT). PDT is a multistep procedure involving the accumulation of a light-sensitive dye known as a photosensitizer (PS) in tumor cells, followed by its activation by irradiation with a light of an appropriate wavelength. In the presence of molecular oxygen, the irradiated PS leads to the generation of cytotoxic reactive oxygen species, which can lead to ICD induction in the cancer cells. Here, we first describe in vitro methods to help optimize the PDT procedure for a specific PS. We also provide a collection of protocols and techniques for assessing ICD in vitro, including analysis of the emission of damage associated molecular patterns (DAMPs), efferocytosis, and the maturation and activation state of antigen presenting cells. Next, we describe in detail protocols for diverse tumor mouse models for assessing and characterizing ICD in vivo, such as murine tumor vaccination models. Finally, as an immunotherapeutic vaccine, we suggest using either PDT-induced dead cancer cells, preferably undergoing ICD, or dendritic cells loaded with lysates of PDT-induced cancer cells in a syngeneic orthotopic glioma model. Overall, this methodological article provides a quantitative, comprehensive set of validated tools that can be successfully used, with some adaptations, to identify, optimize and validate novel PSs in vitro and in vivo for the efficient induction of ICD during photodynamic treatment. Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Keywords
Anti-cancer vaccination, Apoptosis, Calreticulin, Cancer, Cancer immunotherapy, DAMPs, Ferroptosis, Glioblastoma, Glioma, HMGB1, Immunogenic cell death, Methods, Necroptosis, Photodynamic therapy, Tumor mice models, orthotropic glioma mice models, DC vaccines, Vaccines, glioblastoma, ATP

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MLA
Mishchenko, Tatiana A., et al. “Gold Standard Assessment of Immunogenic Cell Death Induced by Photodynamic Therapy : From in Vitro to Tumor Mouse Models and Anti-Cancer Vaccination Strategies.” Cell-Based Cancer Immunotherapy, vol. 183, Elsevier Inc, 2024, pp. 203–64, doi:10.1016/bs.mcb.2023.05.003.
APA
Mishchenko, T. A., Balalaeva, I. V., Turubanova, V. D., Saviuk, M., Shilyagina, N. Y., Krysko, O., … Krysko, D. (2024). Gold standard assessment of immunogenic cell death induced by photodynamic therapy : from in vitro to tumor mouse models and anti-cancer vaccination strategies. In Cell-based Cancer Immunotherapy (Vol. 183, pp. 203–264). https://doi.org/10.1016/bs.mcb.2023.05.003
Chicago author-date
Mishchenko, Tatiana A, Irina V Balalaeva, Victoria D Turubanova, Mariia Saviuk, Natalia Yu Shilyagina, Olga Krysko, Maria V Vedunova, and Dmitri Krysko. 2024. “Gold Standard Assessment of Immunogenic Cell Death Induced by Photodynamic Therapy : From in Vitro to Tumor Mouse Models and Anti-Cancer Vaccination Strategies.” In Cell-Based Cancer Immunotherapy, 183:203–64. Elsevier Inc. https://doi.org/10.1016/bs.mcb.2023.05.003.
Chicago author-date (all authors)
Mishchenko, Tatiana A, Irina V Balalaeva, Victoria D Turubanova, Mariia Saviuk, Natalia Yu Shilyagina, Olga Krysko, Maria V Vedunova, and Dmitri Krysko. 2024. “Gold Standard Assessment of Immunogenic Cell Death Induced by Photodynamic Therapy : From in Vitro to Tumor Mouse Models and Anti-Cancer Vaccination Strategies.” In Cell-Based Cancer Immunotherapy, 183:203–264. Elsevier Inc. doi:10.1016/bs.mcb.2023.05.003.
Vancouver
1.
Mishchenko TA, Balalaeva IV, Turubanova VD, Saviuk M, Shilyagina NY, Krysko O, et al. Gold standard assessment of immunogenic cell death induced by photodynamic therapy : from in vitro to tumor mouse models and anti-cancer vaccination strategies. In: Cell-based Cancer Immunotherapy. Elsevier Inc; 2024. p. 203–64.
IEEE
[1]
T. A. Mishchenko et al., “Gold standard assessment of immunogenic cell death induced by photodynamic therapy : from in vitro to tumor mouse models and anti-cancer vaccination strategies,” in Cell-based Cancer Immunotherapy, vol. 183, Elsevier Inc, 2024, pp. 203–264.
@incollection{01J0B8FDEGX9A9QZV0BY1R3WYG,
  abstract     = {{The discovery of the concept of immunogenic cell death (ICD) is a cornerstone in the development of novel anti-cancer immunotherapeutic approaches. Induction of the ICD pathway by specific anti-cancer therapeutic regimens can eliminate cancer cells by directly killing them during therapy and by activation of strong and specific anti-cancer immunity, leading to a long-lasting immunological memory that prevents cancer recurrence. ICD encompasses different forms of regulated cell death and can be triggered by many anti-cancer treatment modalities, including photodynamic therapy (PDT). PDT is a multistep procedure involving the accumulation of a light-sensitive dye known as a photosensitizer (PS) in tumor cells, followed by its activation by irradiation with a light of an appropriate wavelength. In the presence of molecular oxygen, the irradiated PS leads to the generation of cytotoxic reactive oxygen species, which can lead to ICD induction in the cancer cells. Here, we first describe in vitro methods to help optimize the PDT procedure for a specific PS. We also provide a collection of protocols and techniques for assessing ICD in vitro, including analysis of the emission of damage associated molecular patterns (DAMPs), efferocytosis, and the maturation and activation state of antigen presenting cells. Next, we describe in detail protocols for diverse tumor mouse models for assessing and characterizing ICD in vivo, such as murine tumor vaccination models. Finally, as an immunotherapeutic vaccine, we suggest using either PDT-induced dead cancer cells, preferably undergoing ICD, or dendritic cells loaded with lysates of PDT-induced cancer cells in a syngeneic orthotopic glioma model. Overall, this methodological article provides a quantitative, comprehensive set of validated tools that can be successfully used, with some adaptations, to identify, optimize and validate novel PSs in vitro and in vivo for the efficient induction of ICD during photodynamic treatment. Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.}},
  author       = {{Mishchenko, Tatiana A and  Balalaeva, Irina V and  Turubanova, Victoria D and Saviuk, Mariia and  Shilyagina, Natalia Yu and Krysko, Olga and Vedunova, Maria V and Krysko, Dmitri}},
  booktitle    = {{Cell-based Cancer Immunotherapy}},
  isbn         = {{9780443139956}},
  issn         = {{0091-679X}},
  keywords     = {{Anti-cancer vaccination,Apoptosis,Calreticulin,Cancer,Cancer immunotherapy,DAMPs,Ferroptosis,Glioblastoma,Glioma,HMGB1,Immunogenic cell death,Methods,Necroptosis,Photodynamic therapy,Tumor mice models,orthotropic glioma mice models,DC vaccines,Vaccines,glioblastoma,ATP}},
  language     = {{eng}},
  pages        = {{203--264}},
  publisher    = {{Elsevier Inc}},
  series       = {{Methods in Cell Biology}},
  title        = {{Gold standard assessment of immunogenic cell death induced by photodynamic therapy : from in vitro to tumor mouse models and anti-cancer vaccination strategies}},
  url          = {{http://doi.org/10.1016/bs.mcb.2023.05.003}},
  volume       = {{183}},
  year         = {{2024}},
}

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