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Interaction of whitefly effector G4 with tomato proteins impacts whitefly performance

Diana Naalden (UGent) , Wannes Dermauw (UGent) , Aris Ilias, Geert Baggerman, Marieke Mastop, Juliette J. M. Silven, Paula J. M. van Kleeff, Sarmina Dangol, Nicolas Frederic Gaertner, Winfried Roseboom, et al.
(2024) MOLECULAR PLANT-MICROBE INTERACTIONS. 37(2). p.98-111
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  • SuperPests (Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit)
Abstract
The phloem-feeding insect Bemisia tabaci is an important pest, responsible for the transmission of several crop-threatening virus species. While feeding, the insect secretes a cocktail of effectors to modulate plant defense responses. Here, we present a set of proteins identified in an artificial diet on which B. tabaci was salivating. We subsequently studied whether these candidate effectors can play a role in plant immune suppression. Effector G4 was the most robust suppressor of an induced- reactive oxygen species (ROS) response in Nicotiana benthamiana. In addition, G4 was able to suppress ROS production in Solanum lycopersicum (tomato) and Capsicum annuum (pepper). G4 localized predominantly in the endoplasmic reticulum in N. benthamiana leaves and colocalized with two identified target proteins in tomato: REF-like stress related protein 1 (RSP1) and meloidogyne-induced giant cell protein DB141 (MIPDB141). Silencing of MIPDB141 in tomato reduced whitefly fecundity up to 40%, demonstrating that the protein is involved in susceptibility to B. tabaci. Together, our data demonstrate that effector G4 impairs tomato immunity to whiteflies by interfering with ROS production and via an interaction with tomato susceptibility protein MIPDB141.Copyright (c) 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Keywords
insect resistance, plant defense, plant immune suppression, VIGS, whiteflies, CONTROLLED TUMOR PROTEIN, GATEWAY BINARY VECTORS, BEMISIA-TABACI, RESISTANCE GENE, PHLOEM SAP, PLANT, IDENTIFICATION, LUCIFERASE, EXPRESSION, PROVIDES

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Citation

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MLA
Naalden, Diana, et al. “Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance.” MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 37, no. 2, 2024, pp. 98–111, doi:10.1094/MPMI-04-23-0045-R.
APA
Naalden, D., Dermauw, W., Ilias, A., Baggerman, G., Mastop, M., Silven, J. J. M., … Schuurink, R. C. (2024). Interaction of whitefly effector G4 with tomato proteins impacts whitefly performance. MOLECULAR PLANT-MICROBE INTERACTIONS, 37(2), 98–111. https://doi.org/10.1094/MPMI-04-23-0045-R
Chicago author-date
Naalden, Diana, Wannes Dermauw, Aris Ilias, Geert Baggerman, Marieke Mastop, Juliette J. M. Silven, Paula J. M. van Kleeff, et al. 2024. “Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance.” MOLECULAR PLANT-MICROBE INTERACTIONS 37 (2): 98–111. https://doi.org/10.1094/MPMI-04-23-0045-R.
Chicago author-date (all authors)
Naalden, Diana, Wannes Dermauw, Aris Ilias, Geert Baggerman, Marieke Mastop, Juliette J. M. Silven, Paula J. M. van Kleeff, Sarmina Dangol, Nicolas Frederic Gaertner, Winfried Roseboom, Mark Kwaaitaal, Gertjan Kramer, Harrold A. van den Burg, John Vontas, Thomas Van Leeuwen, Merijn R. Kant, and Robert C. Schuurink. 2024. “Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance.” MOLECULAR PLANT-MICROBE INTERACTIONS 37 (2): 98–111. doi:10.1094/MPMI-04-23-0045-R.
Vancouver
1.
Naalden D, Dermauw W, Ilias A, Baggerman G, Mastop M, Silven JJM, et al. Interaction of whitefly effector G4 with tomato proteins impacts whitefly performance. MOLECULAR PLANT-MICROBE INTERACTIONS. 2024;37(2):98–111.
IEEE
[1]
D. Naalden et al., “Interaction of whitefly effector G4 with tomato proteins impacts whitefly performance,” MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 37, no. 2, pp. 98–111, 2024.
@article{01J08SCXJ5318MSR820F6B343G,
  abstract     = {{The phloem-feeding insect Bemisia tabaci is an important pest, responsible for the transmission of several crop-threatening virus species. While feeding, the insect secretes a cocktail of effectors to modulate plant defense responses. Here, we present a set of proteins identified in an artificial diet on which B. tabaci was salivating. We subsequently studied whether these candidate effectors can play a role in plant immune suppression. Effector G4 was the most robust suppressor of an induced- reactive oxygen species (ROS) response in Nicotiana benthamiana. In addition, G4 was able to suppress ROS production in Solanum lycopersicum (tomato) and Capsicum annuum (pepper). G4 localized predominantly in the endoplasmic reticulum in N. benthamiana leaves and colocalized with two identified target proteins in tomato: REF-like stress related protein 1 (RSP1) and meloidogyne-induced giant cell protein DB141 (MIPDB141). Silencing of MIPDB141 in tomato reduced whitefly fecundity up to 40%, demonstrating that the protein is involved in susceptibility to B. tabaci. Together, our data demonstrate that effector G4 impairs tomato immunity to whiteflies by interfering with ROS production and via an interaction with tomato susceptibility protein MIPDB141.Copyright (c) 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.}},
  author       = {{Naalden, Diana and Dermauw, Wannes and  Ilias, Aris and  Baggerman, Geert and  Mastop, Marieke and  Silven, Juliette J. M. and  van Kleeff, Paula J. M. and  Dangol, Sarmina and  Gaertner, Nicolas Frederic and  Roseboom, Winfried and  Kwaaitaal, Mark and  Kramer, Gertjan and  van den Burg, Harrold A. and  Vontas, John and Van Leeuwen, Thomas and  Kant, Merijn R. and  Schuurink, Robert C.}},
  issn         = {{0894-0282}},
  journal      = {{MOLECULAR PLANT-MICROBE INTERACTIONS}},
  keywords     = {{insect resistance,plant defense,plant immune suppression,VIGS,whiteflies,CONTROLLED TUMOR PROTEIN,GATEWAY BINARY VECTORS,BEMISIA-TABACI,RESISTANCE GENE,PHLOEM SAP,PLANT,IDENTIFICATION,LUCIFERASE,EXPRESSION,PROVIDES}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{98--111}},
  title        = {{Interaction of whitefly effector G4 with tomato proteins impacts whitefly performance}},
  url          = {{http://doi.org/10.1094/MPMI-04-23-0045-R}},
  volume       = {{37}},
  year         = {{2024}},
}
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