Changes in histone lysine acetylation, but not DNA methylation during facultative hibernation in Syrian hamster liver
- Author
- Marloes Oosterhof, Louis Coussement (UGent) , Alienke van Pijkeren, Marcel Kwiatkowski, Martijn R. H. Zwinderman, Frank J. Dekker, Tim De Meyer (UGent) , Vera A. Reitsema, Rainer Bischoff, Victor Guryev, Hjalmar R. Bouma, Rob H. Henning and Marianne G. Rots
- Organization
- Abstract
- Background Hibernation (torpor) is a strategy to survive extreme environmental conditions, associated with a significant decrease in metabolism and body temperature. The inducibility by the environment of torpor for facultative hibernators designates epigenetic mechanisms as likely candidates for regulation. Therefore, we set out to unravel epigenetics in the liver of a facultative hibernator, Syrian hamster (Mesocricetus auratus), sampled at different phases during hibernation, by assessing the expression of epigenetic writer and eraser enzymes, histone acetylation dynamics, and DNA methylation levels. Results Expression of epigenetic writers/erasers confirmed previously reported results obtained in obligatory hibernators, but might point to a mechanism specific for facultative hibernators, e.g., differential expression of histone acetyltransferases (HATs; KAT6A, KAT6B, KAT7, and KAT13D/CLOCK). These findings were in accordance with observed changes in histone H3 and H4 acetylation changes. Overall histone deacetylase (HDAC) activity was highest in torpor. No differences were detected in DNA methylation throughout all phases. Conclusion Our study thus points to histone acetylation as an important player in facultative hamster hibernation, which may underlie the orchestration of gene expression changes throughout hibernation.
- Keywords
- HDAC, HAT, RNA sequencing, Epigenetics, Histone acetylation, WGBS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01J03AQ4W54JP6QJ5DDVWEND11
- MLA
- Oosterhof, Marloes, et al. “Changes in Histone Lysine Acetylation, but Not DNA Methylation during Facultative Hibernation in Syrian Hamster Liver.” EPIGENETICS COMMUNICATIONS, vol. 4, no. 1, 2024, doi:10.1186/s43682-023-00024-2.
- APA
- Oosterhof, M., Coussement, L., van Pijkeren, A., Kwiatkowski, M., Zwinderman, M. R. H., Dekker, F. J., … Rots, M. G. (2024). Changes in histone lysine acetylation, but not DNA methylation during facultative hibernation in Syrian hamster liver. EPIGENETICS COMMUNICATIONS, 4(1). https://doi.org/10.1186/s43682-023-00024-2
- Chicago author-date
- Oosterhof, Marloes, Louis Coussement, Alienke van Pijkeren, Marcel Kwiatkowski, Martijn R. H. Zwinderman, Frank J. Dekker, Tim De Meyer, et al. 2024. “Changes in Histone Lysine Acetylation, but Not DNA Methylation during Facultative Hibernation in Syrian Hamster Liver.” EPIGENETICS COMMUNICATIONS 4 (1). https://doi.org/10.1186/s43682-023-00024-2.
- Chicago author-date (all authors)
- Oosterhof, Marloes, Louis Coussement, Alienke van Pijkeren, Marcel Kwiatkowski, Martijn R. H. Zwinderman, Frank J. Dekker, Tim De Meyer, Vera A. Reitsema, Rainer Bischoff, Victor Guryev, Hjalmar R. Bouma, Rob H. Henning, and Marianne G. Rots. 2024. “Changes in Histone Lysine Acetylation, but Not DNA Methylation during Facultative Hibernation in Syrian Hamster Liver.” EPIGENETICS COMMUNICATIONS 4 (1). doi:10.1186/s43682-023-00024-2.
- Vancouver
- 1.Oosterhof M, Coussement L, van Pijkeren A, Kwiatkowski M, Zwinderman MRH, Dekker FJ, et al. Changes in histone lysine acetylation, but not DNA methylation during facultative hibernation in Syrian hamster liver. EPIGENETICS COMMUNICATIONS. 2024;4(1).
- IEEE
- [1]M. Oosterhof et al., “Changes in histone lysine acetylation, but not DNA methylation during facultative hibernation in Syrian hamster liver,” EPIGENETICS COMMUNICATIONS, vol. 4, no. 1, 2024.
@article{01J03AQ4W54JP6QJ5DDVWEND11,
abstract = {{Background
Hibernation (torpor) is a strategy to survive extreme environmental conditions, associated with a significant decrease in metabolism and body temperature. The inducibility by the environment of torpor for facultative hibernators designates epigenetic mechanisms as likely candidates for regulation. Therefore, we set out to unravel epigenetics in the liver of a facultative hibernator, Syrian hamster (Mesocricetus auratus), sampled at different phases during hibernation, by assessing the expression of epigenetic writer and eraser enzymes, histone acetylation dynamics, and DNA methylation levels.
Results
Expression of epigenetic writers/erasers confirmed previously reported results obtained in obligatory hibernators, but might point to a mechanism specific for facultative hibernators, e.g., differential expression of histone acetyltransferases (HATs; KAT6A, KAT6B, KAT7, and KAT13D/CLOCK). These findings were in accordance with observed changes in histone H3 and H4 acetylation changes. Overall histone deacetylase (HDAC) activity was highest in torpor. No differences were detected in DNA methylation throughout all phases.
Conclusion
Our study thus points to histone acetylation as an important player in facultative hamster hibernation, which may underlie the orchestration of gene expression changes throughout hibernation.}},
articleno = {{1}},
author = {{Oosterhof, Marloes and Coussement, Louis and van Pijkeren, Alienke and Kwiatkowski, Marcel and Zwinderman, Martijn R. H. and Dekker, Frank J. and De Meyer, Tim and Reitsema, Vera A. and Bischoff, Rainer and Guryev, Victor and Bouma, Hjalmar R. and Henning, Rob H. and Rots, Marianne G.}},
issn = {{2730-7034}},
journal = {{EPIGENETICS COMMUNICATIONS}},
keywords = {{HDAC,HAT,RNA sequencing,Epigenetics,Histone acetylation,WGBS}},
language = {{eng}},
number = {{1}},
pages = {{14}},
title = {{Changes in histone lysine acetylation, but not DNA methylation during facultative hibernation in Syrian hamster liver}},
url = {{http://doi.org/10.1186/s43682-023-00024-2}},
volume = {{4}},
year = {{2024}},
}
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