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Prediction of Klebsiella phage-host specificity at the strain level

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Abstract
Phages are increasingly considered promising alternatives to target drug-resistant bacterial pathogens. However, their often-narrow host range can make it challenging to find matching phages against bacteria of interest. Current computational tools do not accurately predict interactions at the strain level in a way that is relevant and properly evaluated for practical use. We present PhageHostLearn, a machine learning system that predicts strain-level interactions between receptor-binding proteins and bacterial receptors for Klebsiella phage-bacteria pairs. We evaluate this system both in silico and in the laboratory, in the clinically relevant setting of finding matching phages against bacterial strains. PhageHostLearn reaches a cross-validated ROC AUC of up to 81.8% in silico and maintains this performance in laboratory validation. Our approach provides a framework for developing and evaluating phage-host prediction methods that are useful in practice, which we believe to be a meaningful contribution to the machine-learning-guided development of phage therapeutics and diagnostics.
Keywords
LANGUAGE, RANGE

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MLA
Boeckaerts, Dimitri, et al. “Prediction of Klebsiella Phage-Host Specificity at the Strain Level.” NATURE COMMUNICATIONS, vol. 15, no. 1, 2024, doi:10.1038/s41467-024-48675-6.
APA
Boeckaerts, D., Stock, M., Ferriol-González, C., Oteo-Iglesias, J., Sanjuán, R., Domingo-Calap, P., … Briers, Y. (2024). Prediction of Klebsiella phage-host specificity at the strain level. NATURE COMMUNICATIONS, 15(1). https://doi.org/10.1038/s41467-024-48675-6
Chicago author-date
Boeckaerts, Dimitri, Michiel Stock, Celia Ferriol-González, Jesús Oteo-Iglesias, Rafael Sanjuán, Pilar Domingo-Calap, Bernard De Baets, and Yves Briers. 2024. “Prediction of Klebsiella Phage-Host Specificity at the Strain Level.” NATURE COMMUNICATIONS 15 (1). https://doi.org/10.1038/s41467-024-48675-6.
Chicago author-date (all authors)
Boeckaerts, Dimitri, Michiel Stock, Celia Ferriol-González, Jesús Oteo-Iglesias, Rafael Sanjuán, Pilar Domingo-Calap, Bernard De Baets, and Yves Briers. 2024. “Prediction of Klebsiella Phage-Host Specificity at the Strain Level.” NATURE COMMUNICATIONS 15 (1). doi:10.1038/s41467-024-48675-6.
Vancouver
1.
Boeckaerts D, Stock M, Ferriol-González C, Oteo-Iglesias J, Sanjuán R, Domingo-Calap P, et al. Prediction of Klebsiella phage-host specificity at the strain level. NATURE COMMUNICATIONS. 2024;15(1).
IEEE
[1]
D. Boeckaerts et al., “Prediction of Klebsiella phage-host specificity at the strain level,” NATURE COMMUNICATIONS, vol. 15, no. 1, 2024.
@article{01HZ6X8V4M84E60Y0XHH62K4TB,
  abstract     = {{Phages are increasingly considered promising alternatives to target drug-resistant bacterial pathogens. However, their often-narrow host range can make it challenging to find matching phages against bacteria of interest. Current computational tools do not accurately predict interactions at the strain level in a way that is relevant and properly evaluated for practical use. We present PhageHostLearn, a machine learning system that predicts strain-level interactions between receptor-binding proteins and bacterial receptors for Klebsiella phage-bacteria pairs. We evaluate this system both in silico and in the laboratory, in the clinically relevant setting of finding matching phages against bacterial strains. PhageHostLearn reaches a cross-validated ROC AUC of up to 81.8% in silico and maintains this performance in laboratory validation. Our approach provides a framework for developing and evaluating phage-host prediction methods that are useful in practice, which we believe to be a meaningful contribution to the machine-learning-guided development of phage therapeutics and diagnostics.

}},
  articleno    = {{4355}},
  author       = {{Boeckaerts, Dimitri and Stock, Michiel and Ferriol-González, Celia and Oteo-Iglesias, Jesús and Sanjuán, Rafael and Domingo-Calap, Pilar and De Baets, Bernard and Briers, Yves}},
  issn         = {{2041-1723}},
  journal      = {{NATURE COMMUNICATIONS}},
  keywords     = {{LANGUAGE,RANGE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{10}},
  title        = {{Prediction of Klebsiella phage-host specificity at the strain level}},
  url          = {{http://doi.org/10.1038/s41467-024-48675-6}},
  volume       = {{15}},
  year         = {{2024}},
}

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