Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS
- Author
- Da Cao (UGent) , Liesl Heughebaert (UGent) , Laura Boffel (UGent) , Christophe Stove (UGent) and Dominique Van Der Straeten (UGent)
- Organization
- Project
-
- Folate stability in rice: a molecular and analytical study
- Biodiversiteit van bodems en landbouwinnovaties voor verbeterde veerkracht in Europese tarwe agrosystemen (‘BIOFAIR’)
- Francqui Research Professorship Dominique Van Der Straeten Understanding plant responses to global climate change at the molecular and physiological level: The role of the plant hormone ethylene & the impact on vitamin metabolism
- Thiamin transport in plants
- Abstract
- B-group vitamins are important micronutrients for maintaining human health; nevertheless, B vitamin deficiency is a globally widespread issue. Thus, it is relevant to accurately assess the B-vitamin content in staple crop products such as wheat grains. Here, we developed a multi-enzyme extraction method allowing accurate quantification of seven B vitamins in wheat using ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Free forms of thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7) and folates (B9) were determined with recoveries ranging from 81 to 118% and accuracy below 15% bias. The precision was below 20% relative standard deviation and the internal standards adequately compensated for matrix effects. The method was applied to determine the B vitamin stabilities in wheat grains stored at different temperatures and periods. The results provide an important basis in future studies aiming at understanding nutritional availability of B vitamins.
- Keywords
- UHPLC-MS/MS, Wheat, Method validation, Quantification, B vitamins, Stability, WATER-SOLUBLE VITAMINS, FOLATE, STABILITY, VALIDATION, BIOTIN, NIACIN, BIOAVAILABILITY, OPTIMIZATION, RIBOFLAVIN, EXTRACTION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HYJG4AGE2B5753P9QA9WN6YH
- MLA
- Cao, Da, et al. “Simultaneous Quantification of Seven B Vitamins from Wheat Grains Using UHPLC-MS/MS.” FOOD CHEMISTRY, vol. 453, 2024, doi:10.1016/j.foodchem.2024.139667.
- APA
- Cao, D., Heughebaert, L., Boffel, L., Stove, C., & Van Der Straeten, D. (2024). Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS. FOOD CHEMISTRY, 453. https://doi.org/10.1016/j.foodchem.2024.139667
- Chicago author-date
- Cao, Da, Liesl Heughebaert, Laura Boffel, Christophe Stove, and Dominique Van Der Straeten. 2024. “Simultaneous Quantification of Seven B Vitamins from Wheat Grains Using UHPLC-MS/MS.” FOOD CHEMISTRY 453. https://doi.org/10.1016/j.foodchem.2024.139667.
- Chicago author-date (all authors)
- Cao, Da, Liesl Heughebaert, Laura Boffel, Christophe Stove, and Dominique Van Der Straeten. 2024. “Simultaneous Quantification of Seven B Vitamins from Wheat Grains Using UHPLC-MS/MS.” FOOD CHEMISTRY 453. doi:10.1016/j.foodchem.2024.139667.
- Vancouver
- 1.Cao D, Heughebaert L, Boffel L, Stove C, Van Der Straeten D. Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS. FOOD CHEMISTRY. 2024;453.
- IEEE
- [1]D. Cao, L. Heughebaert, L. Boffel, C. Stove, and D. Van Der Straeten, “Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS,” FOOD CHEMISTRY, vol. 453, 2024.
@article{01HYJG4AGE2B5753P9QA9WN6YH,
abstract = {{B-group vitamins are important micronutrients for maintaining human health; nevertheless, B vitamin deficiency is a globally widespread issue. Thus, it is relevant to accurately assess the B-vitamin content in staple crop products such as wheat grains. Here, we developed a multi-enzyme extraction method allowing accurate quantification of seven B vitamins in wheat using ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Free forms of thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7) and folates (B9) were determined with recoveries ranging from 81 to 118% and accuracy below 15% bias. The precision was below 20% relative standard deviation and the internal standards adequately compensated for matrix effects. The method was applied to determine the B vitamin stabilities in wheat grains stored at different temperatures and periods. The results provide an important basis in future studies aiming at understanding nutritional availability of B vitamins.}},
articleno = {{139667}},
author = {{Cao, Da and Heughebaert, Liesl and Boffel, Laura and Stove, Christophe and Van Der Straeten, Dominique}},
issn = {{0308-8146}},
journal = {{FOOD CHEMISTRY}},
keywords = {{UHPLC-MS/MS,Wheat,Method validation,Quantification,B vitamins,Stability,WATER-SOLUBLE VITAMINS,FOLATE,STABILITY,VALIDATION,BIOTIN,NIACIN,BIOAVAILABILITY,OPTIMIZATION,RIBOFLAVIN,EXTRACTION}},
language = {{eng}},
pages = {{10}},
title = {{Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS}},
url = {{http://doi.org/10.1016/j.foodchem.2024.139667}},
volume = {{453}},
year = {{2024}},
}
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