Eggs sampling as an effective tool for identifying the incidence of viruses in honey bees involved in artificial queen rearing
- Author
- Caio E. C. Domingues, Laura Šimenc, Ivan Toplak, Dirk de Graaf (UGent) , Lina De Smet (UGent) , Wim Verbeke (UGent) , Luc Peelman (UGent) , Leticia S. Ansaloni and Aleš Gregorc
- Organization
- Project
- Abstract
- The Carniolan honey bee (Apis mellifera carnica) plays an essential role in crop pollination, environment diversity, and the production of honey bee products. However, the health of individual honey bees and their colonies is under pressure due to multiple stressors, including viruses as a significant threat to bees. Monitoring various virus infections could be a crucial selection tool during queen rearing. In the present study, samples from all developmental stages (eggs, larvae, pupae, and queens) were screened for the incidence of seven viruses during queen rearing in Slovenia. The screening of a total of 108 samples from five queen breeders was performed by the RT-qPCR assays. The results showed that the highest incidence was observed for black queen cell virus (BQCV), Lake Sinai virus 3 (LSV3), deformed wing virus B (DWV-B), and sacbrood virus (SBV). The highest viral load was detected in queens (6.07 log10 copies/queen) and larvae (5.50 log10 copies/larva) for BQCV, followed by SBV in larvae (5.47 log10 copies/larva). When comparing all the honey bee developmental stages, the eggs exhibited general screening for virus incidence and load in queen mother colonies. The results suggest that analyzing eggs is a good indicator of resilience to virus infection during queen development.
- Keywords
- DEFORMED-WING-VIRUS, APIS-MELLIFERA, PARALYSIS VIRUS, SACBROOD VIRUS, PHYLOGENETIC ANALYSIS, SEASONAL-VARIATIONS, TRANSMISSION, INFECTIONS, POPULATIONS, PREVALENCE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HYFQAXVCMVHRCPY33Q22EVBE
- MLA
- Domingues, Caio E. C., et al. “Eggs Sampling as an Effective Tool for Identifying the Incidence of Viruses in Honey Bees Involved in Artificial Queen Rearing.” SCIENTIFIC REPORTS, vol. 14, 2024, doi:10.1038/s41598-024-60135-1.
- APA
- Domingues, C. E. C., Šimenc, L., Toplak, I., de Graaf, D., De Smet, L., Verbeke, W., … Gregorc, A. (2024). Eggs sampling as an effective tool for identifying the incidence of viruses in honey bees involved in artificial queen rearing. SCIENTIFIC REPORTS, 14. https://doi.org/10.1038/s41598-024-60135-1
- Chicago author-date
- Domingues, Caio E. C., Laura Šimenc, Ivan Toplak, Dirk de Graaf, Lina De Smet, Wim Verbeke, Luc Peelman, Leticia S. Ansaloni, and Aleš Gregorc. 2024. “Eggs Sampling as an Effective Tool for Identifying the Incidence of Viruses in Honey Bees Involved in Artificial Queen Rearing.” SCIENTIFIC REPORTS 14. https://doi.org/10.1038/s41598-024-60135-1.
- Chicago author-date (all authors)
- Domingues, Caio E. C., Laura Šimenc, Ivan Toplak, Dirk de Graaf, Lina De Smet, Wim Verbeke, Luc Peelman, Leticia S. Ansaloni, and Aleš Gregorc. 2024. “Eggs Sampling as an Effective Tool for Identifying the Incidence of Viruses in Honey Bees Involved in Artificial Queen Rearing.” SCIENTIFIC REPORTS 14. doi:10.1038/s41598-024-60135-1.
- Vancouver
- 1.Domingues CEC, Šimenc L, Toplak I, de Graaf D, De Smet L, Verbeke W, et al. Eggs sampling as an effective tool for identifying the incidence of viruses in honey bees involved in artificial queen rearing. SCIENTIFIC REPORTS. 2024;14.
- IEEE
- [1]C. E. C. Domingues et al., “Eggs sampling as an effective tool for identifying the incidence of viruses in honey bees involved in artificial queen rearing,” SCIENTIFIC REPORTS, vol. 14, 2024.
@article{01HYFQAXVCMVHRCPY33Q22EVBE,
abstract = {{The Carniolan honey bee (Apis mellifera carnica) plays an essential role in crop pollination, environment diversity, and the production of honey bee products. However, the health of individual honey bees and their colonies is under pressure due to multiple stressors, including viruses as a significant threat to bees. Monitoring various virus infections could be a crucial selection tool during queen rearing. In the present study, samples from all developmental stages (eggs, larvae, pupae, and queens) were screened for the incidence of seven viruses during queen rearing in Slovenia. The screening of a total of 108 samples from five queen breeders was performed by the RT-qPCR assays. The results showed that the highest incidence was observed for black queen cell virus (BQCV), Lake Sinai virus 3 (LSV3), deformed wing virus B (DWV-B), and sacbrood virus (SBV). The highest viral load was detected in queens (6.07 log10 copies/queen) and larvae (5.50 log10 copies/larva) for BQCV, followed by SBV in larvae (5.47 log10 copies/larva). When comparing all the honey bee developmental stages, the eggs exhibited general screening for virus incidence and load in queen mother colonies. The results suggest that analyzing eggs is a good indicator of resilience to virus infection during queen development.}},
articleno = {{9612}},
author = {{Domingues, Caio E. C. and Šimenc, Laura and Toplak, Ivan and de Graaf, Dirk and De Smet, Lina and Verbeke, Wim and Peelman, Luc and Ansaloni, Leticia S. and Gregorc, Aleš}},
issn = {{2045-2322}},
journal = {{SCIENTIFIC REPORTS}},
keywords = {{DEFORMED-WING-VIRUS,APIS-MELLIFERA,PARALYSIS VIRUS,SACBROOD VIRUS,PHYLOGENETIC ANALYSIS,SEASONAL-VARIATIONS,TRANSMISSION,INFECTIONS,POPULATIONS,PREVALENCE}},
language = {{eng}},
pages = {{10}},
title = {{Eggs sampling as an effective tool for identifying the incidence of viruses in honey bees involved in artificial queen rearing}},
url = {{http://doi.org/10.1038/s41598-024-60135-1}},
volume = {{14}},
year = {{2024}},
}
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