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A precise balance of TETRASPANIN1/TORNADO2 activity is required for vascular proliferation and ground tissue patterning in Arabidopsis

Nataliia Konstantinova (UGent) , Eliana Mor, Eline Verhelst (UGent) , Jonah Nolf (UGent) , Kenzo Vereecken (UGent) , Feng Wang (UGent) , Daniël Van Damme (UGent) , Bert De Rybel (UGent) and Matous Glanc (UGent)
(2024) PHYSIOLOGIA PLANTARUM. 176(1).
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Abstract
The molecular mechanisms guiding oriented cell divisions in the root vascular tissues of Arabidopsis thaliana are still poorly characterised. By overlapping bulk and single-cell transcriptomic datasets, we unveiled TETRASPANIN1 (TET1) as a putative regulator in this process. TET1 is expressed in root vascular cells, and loss-of-function mutants contain fewer vascular cell files. We further generated and characterised a CRISPR deletion mutant and showed, unlike previously described mutants, that the full knock out is additionally missing endodermal cells in a stochastic way. Finally, we show that HA-tagged versions of TET1 are functional in contrast to fluorescent TET1 translational fusions. Immunostaining using HA-TET1 lines complementing the mutant phenotype suggested a dual plasma membrane and intracellular localisation in the root vasculature and a polar membrane localisation in the young cortex, endodermal and initial cells. Taken together, we show that TET1 is involved in both vascular proliferation and ground tissue patterning. Our initial results pave the way for future work to decipher its precise mode of action.

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MLA
Konstantinova, Nataliia, et al. “A Precise Balance of TETRASPANIN1/TORNADO2 Activity Is Required for Vascular Proliferation and Ground Tissue Patterning in Arabidopsis.” PHYSIOLOGIA PLANTARUM, vol. 176, no. 1, 2024, doi:10.1111/ppl.14182.
APA
Konstantinova, N., Mor, E., Verhelst, E., Nolf, J., Vereecken, K., Wang, F., … Glanc, M. (2024). A precise balance of TETRASPANIN1/TORNADO2 activity is required for vascular proliferation and ground tissue patterning in Arabidopsis. PHYSIOLOGIA PLANTARUM, 176(1). https://doi.org/10.1111/ppl.14182
Chicago author-date
Konstantinova, Nataliia, Eliana Mor, Eline Verhelst, Jonah Nolf, Kenzo Vereecken, Feng Wang, Daniël Van Damme, Bert De Rybel, and Matous Glanc. 2024. “A Precise Balance of TETRASPANIN1/TORNADO2 Activity Is Required for Vascular Proliferation and Ground Tissue Patterning in Arabidopsis.” PHYSIOLOGIA PLANTARUM 176 (1). https://doi.org/10.1111/ppl.14182.
Chicago author-date (all authors)
Konstantinova, Nataliia, Eliana Mor, Eline Verhelst, Jonah Nolf, Kenzo Vereecken, Feng Wang, Daniël Van Damme, Bert De Rybel, and Matous Glanc. 2024. “A Precise Balance of TETRASPANIN1/TORNADO2 Activity Is Required for Vascular Proliferation and Ground Tissue Patterning in Arabidopsis.” PHYSIOLOGIA PLANTARUM 176 (1). doi:10.1111/ppl.14182.
Vancouver
1.
Konstantinova N, Mor E, Verhelst E, Nolf J, Vereecken K, Wang F, et al. A precise balance of TETRASPANIN1/TORNADO2 activity is required for vascular proliferation and ground tissue patterning in Arabidopsis. PHYSIOLOGIA PLANTARUM. 2024;176(1).
IEEE
[1]
N. Konstantinova et al., “A precise balance of TETRASPANIN1/TORNADO2 activity is required for vascular proliferation and ground tissue patterning in Arabidopsis,” PHYSIOLOGIA PLANTARUM, vol. 176, no. 1, 2024.
@article{01HYEB3A4JFBGXS0P2VDB6RW22,
  abstract     = {{The molecular mechanisms guiding oriented cell divisions in the root vascular tissues of Arabidopsis thaliana are still poorly characterised. By overlapping bulk and single-cell transcriptomic datasets, we unveiled TETRASPANIN1 (TET1) as a putative regulator in this process. TET1 is expressed in root vascular cells, and loss-of-function mutants contain fewer vascular cell files. We further generated and characterised a CRISPR deletion mutant and showed, unlike previously described mutants, that the full knock out is additionally missing endodermal cells in a stochastic way. Finally, we show that HA-tagged versions of TET1 are functional in contrast to fluorescent TET1 translational fusions. Immunostaining using HA-TET1 lines complementing the mutant phenotype suggested a dual plasma membrane and intracellular localisation in the root vasculature and a polar membrane localisation in the young cortex, endodermal and initial cells. Taken together, we show that TET1 is involved in both vascular proliferation and ground tissue patterning. Our initial results pave the way for future work to decipher its precise mode of action.}},
  articleno    = {{e14182}},
  author       = {{Konstantinova, Nataliia and Mor, Eliana and Verhelst, Eline and Nolf, Jonah and Vereecken, Kenzo and Wang, Feng and Van Damme, Daniël and De Rybel, Bert and Glanc, Matous}},
  issn         = {{0031-9317}},
  journal      = {{PHYSIOLOGIA PLANTARUM}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{12}},
  title        = {{A precise balance of TETRASPANIN1/TORNADO2 activity is required for vascular proliferation and ground tissue patterning in Arabidopsis}},
  url          = {{http://doi.org/10.1111/ppl.14182}},
  volume       = {{176}},
  year         = {{2024}},
}
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