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Lesion depth on thoracic ultrasound in relation to isolated pathogens from bronchoalveolar lavage fluid in calves

Justine Clinquart (UGent) , Thomas Lowie (UGent) , Stan Jourquin (UGent) , Florian Debruyne (UGent) , Jade Bokma (UGent) and Bart Pardon (UGent)
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Abstract
Background : In the context of antimicrobial resistance, the treatment of common respiratory diseases in youngstock should be rationalized as much as possible. The bovine respiratory disease complex groups diseases caused by different etiologies. However, only the treatment of pneumonia with involvement of a bacterial etiology (primary or bacterial superinfection) can be considered rational use. Methods : A cross-sectional study was performed on Belgian farms (dairy, meat and veal) reporting a respiratory outbreak. On these farms a clinical examination, a thoracic ultrasound and a non-bronchoscopic bronchoalveolar lavage was performed on 5 tot 20 calves. Cytology, bacteriology and nanopore sequencing were performed. Following, associations were researched by multilevel logistic regression. Results : Calves with a lesion depth of 1 cm - 2.5 cm and ≥ 3 cm on ultrasound had a higher odds (ORR = 5.33, P = 0.001 & ORR = 8.53, P = 0.001) on having a bacterial superinfection (dominant culture of Pasteurellacae sensu latu, Moraxella spp. or Trueperella pyogenes, and a neutrophilic profile) than calves with no lesions. However calves with lesions of < 1 cm did not have a significantly higher odds than calves without lesions (ORR - 1.91, P = 0.628). No associations were found between the presence of a viral pathogen or M. bovis and the different groups of lesion depth (no lesion, < 1 cm, 1 cm - 2.5 cm ≥ 3 cm). In addition, no association was found between clinical illness and the different etiologies. Conclusions : A clear association was found between sonographic lesions of ≥ 1 cm and the presence of a bacterial superinfection. Hence, this seems a better first line criterion for antimicrobial treatment than clinical illness.
Keywords
youngstock, respiratory diseases, thoracic ultrasound, lesion depth

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MLA
Clinquart, Justine, et al. “Lesion Depth on Thoracic Ultrasound in Relation to Isolated Pathogens from Bronchoalveolar Lavage Fluid in Calves.” Proceedings of the 23rd Middle-European Buiatrics Congress, 2024, pp. 11–11.
APA
Clinquart, J., Lowie, T., Jourquin, S., Debruyne, F., Bokma, J., & Pardon, B. (2024). Lesion depth on thoracic ultrasound in relation to isolated pathogens from bronchoalveolar lavage fluid in calves. Proceedings of the 23rd Middle-European Buiatrics Congress, 11–11.
Chicago author-date
Clinquart, Justine, Thomas Lowie, Stan Jourquin, Florian Debruyne, Jade Bokma, and Bart Pardon. 2024. “Lesion Depth on Thoracic Ultrasound in Relation to Isolated Pathogens from Bronchoalveolar Lavage Fluid in Calves.” In Proceedings of the 23rd Middle-European Buiatrics Congress, 11–11.
Chicago author-date (all authors)
Clinquart, Justine, Thomas Lowie, Stan Jourquin, Florian Debruyne, Jade Bokma, and Bart Pardon. 2024. “Lesion Depth on Thoracic Ultrasound in Relation to Isolated Pathogens from Bronchoalveolar Lavage Fluid in Calves.” In Proceedings of the 23rd Middle-European Buiatrics Congress, 11–11.
Vancouver
1.
Clinquart J, Lowie T, Jourquin S, Debruyne F, Bokma J, Pardon B. Lesion depth on thoracic ultrasound in relation to isolated pathogens from bronchoalveolar lavage fluid in calves. In: Proceedings of the 23rd Middle-European buiatrics congress. 2024. p. 11–11.
IEEE
[1]
J. Clinquart, T. Lowie, S. Jourquin, F. Debruyne, J. Bokma, and B. Pardon, “Lesion depth on thoracic ultrasound in relation to isolated pathogens from bronchoalveolar lavage fluid in calves,” in Proceedings of the 23rd Middle-European buiatrics congress, Brno, 2024, pp. 11–11.
@inproceedings{01HY1EMHGR5JT5RW8KXJJGN9HK,
  abstract     = {{Background : 
In the context of antimicrobial resistance, the treatment of common respiratory diseases in youngstock should be rationalized as much as possible. The bovine respiratory disease complex groups diseases caused by different etiologies. However, only the treatment of pneumonia with involvement of a bacterial etiology (primary or bacterial superinfection) can be considered rational use.

Methods : 
A cross-sectional study was performed on Belgian farms (dairy, meat and veal) reporting a respiratory outbreak. On these farms a clinical examination, a thoracic ultrasound and a non-bronchoscopic bronchoalveolar lavage was performed on 5 tot 20 calves. Cytology, bacteriology and nanopore sequencing were performed. Following, associations were researched by multilevel logistic regression.

Results : 
Calves with a lesion depth of 1 cm - 2.5 cm and ≥ 3 cm on ultrasound had a higher odds (ORR = 5.33, P = 0.001 & ORR = 8.53, P = 0.001) on having a bacterial superinfection (dominant culture of Pasteurellacae sensu latu, Moraxella spp. or Trueperella pyogenes, and a neutrophilic profile) than calves with no lesions. However calves with lesions of < 1 cm did not have a significantly higher odds than calves without lesions (ORR - 1.91, P = 0.628). No associations were found between the presence of a viral pathogen or M. bovis and the different groups of lesion depth (no lesion, < 1 cm, 1 cm - 2.5 cm ≥ 3 cm). In addition, no association was found between clinical illness and the different etiologies.

Conclusions : 
A clear association was found between sonographic lesions of ≥ 1 cm  and the presence of a bacterial superinfection. Hence, this seems a better first line criterion for antimicrobial treatment than clinical illness.}},
  author       = {{Clinquart, Justine and Lowie, Thomas and Jourquin, Stan and Debruyne, Florian and Bokma, Jade and Pardon, Bart}},
  booktitle    = {{Proceedings of the 23rd Middle-European buiatrics congress}},
  keywords     = {{youngstock,respiratory diseases,thoracic ultrasound,lesion depth}},
  language     = {{eng}},
  location     = {{Brno}},
  pages        = {{11--11}},
  title        = {{Lesion depth on thoracic ultrasound in relation to isolated pathogens from bronchoalveolar lavage fluid in calves}},
  year         = {{2024}},
}